Western blot, also called an immunoblot, is an analytical assay commonly used to determine the presence of a particular protein and quantify it. The process uses polyacrylamide gel electrophoresis (PAGE) to separate proteins from a heterogenous lysate based on their molecular weight or 3D structure. The separated proteins are then transferred to a PVDF or nitrocellulose membrane, where the protein(s) of interest can be detected using highly specific antibodies. The detection can then be visualized using chromogenic, fluorescent, or chemiluminescent reporters. Since its initial discovery in the late 1970’s, this sensitive visual assay has become an invaluable tool for molecular biology and proteomics researchers.
Novus offers a range of support options for western blot when you buy a product to help you with your western blot experiment including:
![Western Blot: Kir6.1 Overexpression Lysate (Native) [NBL1-12174] - 1. Negative control, 2. HEK cells overexpressing Kir6.1. 15ug lysate each was loaded onto a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane, and incubated with an anti-Kir6.1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Kir6-1-Overexpression-Lysate-Native-Western-Blot-NBL1-12174-img0003.jpg "Western Blot: Kir6.1 Overexpression Lysate (Native) [NBL1-12174] - 1. Negative control, 2. HEK cells overexpressing Kir6.1. 15ug lysate each was loaded onto a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane, and incubated with an anti-Kir6.1 antibody. Image from verified customer review.")
![Western Blot: Kir6.1 Overexpression Lysate (Adult Normal) [NBL1-12174] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Kir6.1.](http://images.novusbio.com/fullsize/Kir6-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12174-img0002.jpg)
![Western Blot: SLC44A2 Overexpression Lysate (Adult Normal) [NBL1-16161] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC44A2.](http://images.novusbio.com/fullsize/SLC44A2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16161-img0002.jpg "Western Blot: SLC44A2 Overexpression Lysate (Adult Normal) [NBL1-16161] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC44A2.")
![Western Blot: Nucleophosmin Overexpression Lysate [NBL1-13748] - Analysis of Nucleophosmin Overexpression Lysate using Nucleophosmin antibody as a positive control. Image provided via product review by verified customer.](http://images.novusbio.com/fullsize/Nucleophosmin-Overexpression-Lysate-Western-Blot-NBL1-13748-img0003.jpg "Western Blot: Nucleophosmin Overexpression Lysate [NBL1-13748] - Analysis of Nucleophosmin Overexpression Lysate using Nucleophosmin antibody as a positive control. Image provided via product review by verified customer.")
![Western Blot: Nucleophosmin Overexpression Lysate (Adult Normal) [NBL1-13748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleophosmin.](http://images.novusbio.com/fullsize/Nucleophosmin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13748-img0002.jpg)
![Western Blot: TMPRSS4 Overexpression Lysate [NBP2-04784] - (Native) [NBP2-04784] - TMPRSS4 detection with empty vector and overexpression lysate. Image from verified customer review. The observed molecular weight is represented by the band at approximately 48 kDa.](http://images.novusbio.com/fullsize/TMPRSS4-Overexpression-Lysate-Native-Western-Blot-NBP2-04784-img0002.jpg "Western Blot: TMPRSS4 Overexpression Lysate [NBP2-04784] - (Native) [NBP2-04784] - TMPRSS4 detection with empty vector and overexpression lysate. Image from verified customer review. The observed molecular weight is represented by the band at approximately 48 kDa.")
![Western Blot: TMPRSS4 Overexpression Lysate [NBP2-04784] - (Adult Normal) [NBP2-04784] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TMPRSS4. The observed molecular weight is approximately 40 kDa.](http://images.novusbio.com/fullsize/TMPRSS4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04784-img0001.jpg)
![Western Blot: EMP2 Overexpression Lysate (Native) [NBL1-10260] - analysis of EMP2 in EMP2 Overexpression Lysate using anti-EMP2 antibody (Cat.# NBP1-86847, 1:500 dilution). Image from verified customer review.](http://images.novusbio.com/fullsize/EMP2-Overexpression-Lysate-Native-Western-Blot-NBL1-10260-img0003.jpg "Western Blot: EMP2 Overexpression Lysate (Native) [NBL1-10260] - analysis of EMP2 in EMP2 Overexpression Lysate using anti-EMP2 antibody (Cat.# NBP1-86847, 1:500 dilution). Image from verified customer review.")
![Western Blot: EMP2 Overexpression Lysate (Adult Normal) [NBL1-10260] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EMP2.](http://images.novusbio.com/fullsize/EMP2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10260-img0002.jpg)
![Western Blot: VSIG1 Overexpression Lysate (Native) [NBL1-17761] - VSIG1 expression in vector only transfected lysate (left) and VSIG1 over-expression lysate (right) using anti-VSIG1 antibody (Cat.# NBP1-81074). Image from verified customer review.](http://images.novusbio.com/fullsize/VSIG1-Overexpression-Lysate-Native-Western-Blot-NBL1-17761-img0003.jpg "Western Blot: VSIG1 Overexpression Lysate (Native) [NBL1-17761] - VSIG1 expression in vector only transfected lysate (left) and VSIG1 over-expression lysate (right) using anti-VSIG1 antibody (Cat.# NBP1-81074). Image from verified customer review.")
![Western Blot: VSIG1 Overexpression Lysate (Adult Normal) [NBL1-17761] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VSIG1.](http://images.novusbio.com/fullsize/VSIG1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17761-img0002.jpg)
![Western Blot: CD20 Overexpression Lysate (Native) [NBL1-13312] - Detection of CD20 in lymphoma cell line lysates. CD20 over expression lysate was used as the positive control. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/CD20-Overexpression-Lysate-Native-Western-Blot-NBL1-13312-img0003.jpg "Western Blot: CD20 Overexpression Lysate (Native) [NBL1-13312] - Detection of CD20 in lymphoma cell line lysates. CD20 over expression lysate was used as the positive control. This image was submitted via customer Review.")
![Western Blot: CD20 Overexpression Lysate (Native) [NBL1-13312] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD20.](http://images.novusbio.com/fullsize/CD20-Overexpression-Lysate-Native-Western-Blot-NBL1-13312-img0002.jpg)
![Western Blot: PHD3/HIF Prolyl Hydroxylase 3 Overexpression Lysate (Adult Normal) [NBL1-10156] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for HIF Prolyl Hydroxylase 3.](http://images.novusbio.com/fullsize/PHD3-HIF-Prolyl-Hydroxylase-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10156-img0003.jpg "Western Blot: PHD3/HIF Prolyl Hydroxylase 3 Overexpression Lysate (Adult Normal) [NBL1-10156] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for HIF Prolyl Hydroxylase 3.")
![Western Blot: RALBP1 Overexpression Lysate (Adult Normal) [NBL1-15132] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RALBP1.](http://images.novusbio.com/fullsize/RALBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15132-img0002.jpg "Western Blot: RALBP1 Overexpression Lysate (Adult Normal) [NBL1-15132] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RALBP1.")
![Western Blot: MC1 Receptor Overexpression Lysate (Adult Normal) [NBL1-12937] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for MC1 Receptor.](http://images.novusbio.com/fullsize/MC1-Receptor-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12937-img0003.jpg "Western Blot: MC1 Receptor Overexpression Lysate (Adult Normal) [NBL1-12937] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for MC1 Receptor.")
![Western Blot: alpha-N-acetylgalactosaminidase/NAGA Overexpression Lysate [NBL1-13462] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NAGA.](http://images.novusbio.com/fullsize/NAGA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13462-img0002.jpg "Western Blot: alpha-N-acetylgalactosaminidase/NAGA Overexpression Lysate [NBL1-13462] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NAGA.")
![Western Blot: MC2 Receptor Overexpression Lysate (Adult Normal) [NBP2-04160] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MC2 Receptor.](http://images.novusbio.com/fullsize/MC2-Receptor-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04160-img0001.jpg "Western Blot: MC2 Receptor Overexpression Lysate (Adult Normal) [NBP2-04160] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MC2 Receptor.")