Kir6.1 Overexpression Lysate

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Western Blot: Kir6.1 Overexpression Lysate (Native) [NBL1-12174] - 1. Negative control, 2. HEK cells overexpressing Kir6.1. 15ug lysate each was loaded onto a 10% SDS-polyacrylamide gel and transferred to a PVDF ...read more
Western Blot: Kir6.1 Overexpression Lysate (Adult Normal) [NBL1-12174] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Kir6.1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Kir6.1 Overexpression Lysate Summary

Description

Kir6.1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC200340

Accession#: NM_004982

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
KCNJ8

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
47.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBL1-12174 in the following application:

Publications
Read Publication using
NBL1-12174 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Kir6.1 Overexpression Lysate

  • ATP-sensitive inward rectifier potassium channel 8
  • Inward rectifier K(+) channel Kir6.1
  • inwardly rectifying potassium channel KIR6.1
  • KCNJ8
  • Kir6.1
  • Potassium channel, inwardly rectifying subfamily J member 8
  • potassium inwardly-rectifying channel, subfamily J, member 8
  • uKATP-1

Background

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Kir6.1 Lysate (NBL1-12174)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for Kir6.1 Lysate (NBL1-12174) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBL1-12174:
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 Kir6.1 NBL1-12174
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5
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Verified Customer
05/08/2018
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Summary

Lot2DF6DE

Comments

Comments1. Negative control
2. HEK cells overexpressing Kir6.1

Western blot where 15µg of each lysate was loaded onto a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane, later incubated with an anti-Kir6.1 antibody.

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05/08/2018
Application:
Species:

Bioinformatics

Gene Symbol KCNJ8