TMPRSS4 Overexpression Lysate (Native)

Images

 
Western Blot: TMPRSS4 Overexpression Lysate (Native) [NBP2-04784] - TMPRSS4 detection with empty vector and overexpression lysate. Image from verified customer review.
Western Blot: TMPRSS4 Overexpression Lysate (Adult Normal) [NBP2-04784] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TMPRSS4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TMPRSS4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_899070. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens TMPRSS4, mRNA.
Gene
TMPRSS4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Reviewed Applications
Read 1 Review rated 5
using
NBP2-04784 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TMPRSS4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TMPRSS4 Overexpression Lysate (Native)

  • CAPH2
  • Channel-activating protease 2
  • EC 3.4.21
  • EC 3.4.21.4
  • EC 3.4.21.43
  • Membrane-type serine protease 2
  • MT-SP2type II membrane serine protease
  • TMPRSS3EC 3.4.21.-
  • transmembrane protease serine 4
  • transmembrane protease, serine 4
  • transmembrane serine protease 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Review for TMPRSS4 Lysate (NBP2-04784) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-04784:
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Images Ratings Applications Species Date Details
Western Blot TMPRSS4 NBP2-04784
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reviewed by:
Ming Lu
WB Human 08/15/2014
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Summary

ApplicationWestern Blot
Sample Testedlysates from Novusbio Cat# NBP2-04784
SpeciesHuman
Lot2EE45A
CommentsI had trouble in blotting TMPRSS4 in cancer cell lysates. I did not know it is because the antibody I used was a bad one, or because my cells did not express any TMPRSS4, until I found this positive control for TMPRSS4. Novusbio offers empty vector transfected and TMPRSS4 transfected cell lysate, the later shows strong band at the correct molecular weight.

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:1000 overnight at 4C in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#Cell Signaling, #7074
Secondary Concentration1:5000

Details

Detection Notes1 min exposure gives strong band at correct MW.

Comments

CommentsI had trouble in blotting TMPRSS4 in cancer cell lysates. I did not know it is because the antibody I used was a bad one, or because my cells did not express any TMPRSS4, until I found this positive control for TMPRSS4. Novusbio offers empty vector transfected and TMPRSS4 transfected cell lysate, the later shows strong band at the correct molecular weight.

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Video Protocols

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FAQs for TMPRSS4 Lysate (NBP2-04784) (0)

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Recent Reviews

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Ming Lu
08/15/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol TMPRSS4