SLC44A2 Overexpression Lysate (Native)

Images

 
Western Blot: SLC44A2 Overexpression Lysate (Adult Normal) [NBL1-16161] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC44A2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SLC44A2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207181 and based on accession number NM_020428. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens solute carrier family 44, member 2 (SLC44A2), transcript variant 1, mRNA.
Gene
SLC44A2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
79.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBL1-16161 in the following application:

Publications
Read Publications using NBL1-16161.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SLC44A2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SLC44A2 Overexpression Lysate (Native)

  • CTL2choline transporter-like protein 2
  • DKFZp666A071
  • FLJ44586
  • PP1292
  • Solute carrier family 44 member 2
  • solute carrier family 44, member 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for SLC44A2 Lysate (NBL1-16161)(2)

Review for SLC44A2 Lysate (NBL1-16161) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBL1-16161:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot SLC44A2 NBL1-16161
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reviewed by:
Masato Inazu
WB Human 07/18/2015
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Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

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Recent Reviews

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1
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Masato Inazu
07/18/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol SLC44A2