Western Blot: VSIG1 Overexpression Lysate (Native) [NBL1-17761] - VSIG1 expression in vector only transfected lysate (left) and VSIG1 over-expression lysate (right) using anti-VSIG1 antibody (Cat.# NBP1-81074). Image ...read more
Western Blot: VSIG1 Overexpression Lysate (Adult Normal) [NBL1-17761] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VSIG1.
The lysate was created in HEK293T cells, using plasmid ID RC207391 and based on accession number NM_182607. The protein contains a C-terminal DDK tag.
Homo sapiens V-set and immunoglobulin domain containing 1 (VSIG1), mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
39.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBL1-17761 in the following application:
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for VSIG1 Overexpression Lysate (Native)
Cell surface A33 antigen
V-set and immunoglobulin domain containing 1
V-set and immunoglobulin domain-containing protein 1
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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