VSIG1 Overexpression Lysate (Native)

Western Blot: VSIG1 Overexpression Lysate (Native) [NBL1-17761] - VSIG1 expression in vector only transfected lysate (left) and VSIG1 over-expression lysate (right) using anti-VSIG1 antibody (Cat.# NBP1-81074). Image from verified customer review.

Western Blot: VSIG1 Overexpression Lysate (Adult Normal) [NBL1-17761] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VSIG1.

• Reactivity: Hu
• Applications: WB

VSIG1 Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC207391 and based on accession number NM_182607. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens V-set and immunoglobulin domain containing 1 (VSIG1), mRNA.
• Gene: VSIG1

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 39.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for VSIG1 Overexpression Lysate (Native)

• Cell surface A33 antigen
• dJ889N15.1
• Glycoprotein A34
• GPA34
• MGC44287,1700062D20Rik
• V-set and immunoglobulin domain containing 1
• V-set and immunoglobulin domain-containing protein 1
• VSIG1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Review for VSIG1 Lysate (NBL1-17761) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ming Lu WB Human 09/26/2014

Summary

• Application: Western Blot
• Sample Tested: VSIG1 overepxression lysate
• Species: Human
• Lot: 2F27EE
• Comments: This item comes with a pair--one empty vector and one VSIG1 transfected lysates. It works great as negative and positive controls for VSIG1 western blot.

Blocking

• Blocking Details: 2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

• Dilution Ratio: Novus NBP1-81074 1:1000 overnight at 4C in blocking buffer

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG, HRP
• Secondary Manufacturer Cat#: Cell Signaling, #7074
• Secondary Concentration: 1:5000

Details

• Detection Notes: 1 min exposure gives band at correct MW.

Comments

• Comments: This item comes with a pair--one empty vector and one VSIG1 transfected lysates. It works great as negative and positive controls for VSIG1 western blot.

Bioinformatics

• Gene Symbol: VSIG1