VSIG1 Overexpression Lysate (Native)

Images

 
Western Blot: VSIG1 Overexpression Lysate (Native) [NBL1-17761] - VSIG1 expression in vector only transfected lysate (left) and VSIG1 over-expression lysate (right) using anti-VSIG1 antibody (Cat.# NBP1-81074). Image ...read more
Western Blot: VSIG1 Overexpression Lysate (Adult Normal) [NBL1-17761] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VSIG1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

VSIG1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207391 and based on accession number NM_182607. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens V-set and immunoglobulin domain containing 1 (VSIG1), mRNA.
Gene
VSIG1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
39.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBL1-17761 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for VSIG1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for VSIG1 Overexpression Lysate (Native)

  • Cell surface A33 antigen
  • dJ889N15.1
  • Glycoprotein A34
  • GPA34
  • MGC44287,1700062D20Rik
  • V-set and immunoglobulin domain containing 1
  • V-set and immunoglobulin domain-containing protein 1
  • VSIG1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for VSIG1 Lysate (NBL1-17761) (0)

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Review for VSIG1 Lysate (NBL1-17761) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBL1-17761:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot VSIG1 NBL1-17761
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reviewed by:
Ming Lu
WB Human 09/26/2014
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Summary

ApplicationWestern Blot
Sample TestedVSIG1 overepxression lysate
SpeciesHuman
Lot2F27EE
CommentsThis item comes with a pair--one empty vector and one VSIG1 transfected lysates. It works great as negative and positive controls for VSIG1 western blot.

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution RatioNovus NBP1-81074 1:1000 overnight at 4C in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#Cell Signaling, #7074
Secondary Concentration1:5000

Details

Detection Notes1 min exposure gives band at correct MW.

Comments

CommentsThis item comes with a pair--one empty vector and one VSIG1 transfected lysates. It works great as negative and positive controls for VSIG1 western blot.

Product General Protocols

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Video Protocols

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FAQs for VSIG1 Lysate (NBL1-17761) (0)

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Bioinformatics Tool for VSIG1 Lysate (NBL1-17761)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Ming Lu
09/26/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol VSIG1
Entrez