Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56740SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56148SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22444SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-294-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-614SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2732SS | |||
| Azide and BSA Free | NBP2-80779 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1828SS | |||
| Azide and BSA Free | NBP2-80880 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB430-SP | |||
| Carrier Free | MAB430-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-1921SS | |||
![Western Blot: TRAF3IP2 Antibody [NB100-56740] - Analysis of CIKS using NB100-56740 on 15 ugs of mouse kidney cell lysate.](http://images.novusbio.com/fullsize/TRAF3IP2-Antibody-Western-Blot-NB100-56740-img0001.jpg "Western Blot: TRAF3IP2 Antibody [NB100-56740] - Analysis of CIKS using NB100-56740 on 15 ugs of mouse kidney cell lysate.")
![Immunohistochemistry-Paraffin: TRAF3IP2 Antibody [NB100-56740] - Analysis of ACT1 in human kidney tissue using ACT1 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/TRAF3IP2-Antibody-Immunohistochemistry-Paraffin-NB100-56740-img0003.jpg)
![Western Blot: TRAF3IP2 Antibody [NB100-56740] - Analysis of ACT1 in human kidney lysate in the A) absence and B) presence of immunizing peptide using ACT1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TRAF3IP2-Antibody-Western-Blot-NB100-56740-img0002.jpg)
![Simple Western: TRAF3IP2 Antibody [NB100-56740] - Simple Western lane view shows a specific band for TRAF3IP2 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TRAF3IP2-Antibody-Simple-Western-NB100-56740-img0004.jpg)
![Immunohistochemistry: NLRP1/NALP1 Antibody [NB100-56148] - OHT-induced upregulation of Aim2 inflammasome in the retina. NLRP1 antibody labeling (red) in the inner retina; yellow arrows indicate colocalization with RGC neurons (RBPSM+) in the GCL. Image collected and cropped by Citeab from the following publication (Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury. Front Mol Neurosci (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NLRP1-NALP1-Antibody-Immunohistochemistry-NB100-56148-img0010.jpg "Immunohistochemistry: NLRP1/NALP1 Antibody [NB100-56148] - OHT-induced upregulation of Aim2 inflammasome in the retina. NLRP1 antibody labeling (red) in the inner retina; yellow arrows indicate colocalization with RGC neurons (RBPSM+) in the GCL. Image collected and cropped by Citeab from the following publication (Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury. Front Mol Neurosci (2019) licensed under a CC-BY license.")
![Western Blot: NLRP1/NALP1 Antibody [NB100-56148] - Analysis of NLRP1/NALP1 in adult human tissue lysates using NLRP1 antibody at 1:2000. Full-length NLRP1 protein was detected at highest levels in kidney, brain and epidermis. A smaller band was detected in thymus, possibly representing a NLRP1/NALP1 breakdown product or alternatively spliced form. 293T/NALP1: positive control, 293T cells transiently transfected with full-length NLRP1.](http://images.novusbio.com/fullsize/NLRP1-NALP1-Antibody-Western-Blot-NB100-56148-img0007.jpg)
![Immunohistochemistry: NLRP1/NALP1 Antibody [NB100-56148] - The NLRP1and NLRP3 proteins localized to distinct cell types in the GCL of naive and NT control retinas: NLRP3 (green) is only expressed in blood vessels (white arrowheads). Twenty four hours after OHT, NLRP3 colocalizes with NLRP1 in RGCs (yellow arrows) and to RBPMS- cells (white arrows). Blue arrowheads denote labeling in RGC axons and dendrites. Bar, 25 um on all panels. Image collected and cropped by Citeab from the following publication (Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury. Front Mol Neurosci (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NLRP1-NALP1-Antibody-Immunohistochemistry-NB100-56148-img0011.jpg)
![Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56148] - FFPE normal human tissue sections stained for NALP1 (CARD7/NAC) using NB100-56148 at 1:2000. (A) epidermis, (B) esophagus (C) uterine cervix, and (D) thymus. Hematoxylin-eosin counterstain. In A-C, NALP1 expression is associated with differentiation in stratified epithelial of the skin, esophagus, and cervix.](http://images.novusbio.com/fullsize/NLRP1-NALP1-Antibody-Immunohistochemistry-Paraffin-NB100-56148-img0005.jpg)
![Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56148] - FFPE human tissue sections stained for NALP1 (CARD7/NAC) using NB100-56148 at 1:2000. (A) bone marrow and (C) brain glioma core from a brain cancer microarray. (B) and (D) are higher magnifications of (A) and (C) respectively. Hemaxotylin-eosin counterstain.](http://images.novusbio.com/fullsize/NLRP1-NALP1-Antibody-Immunohistochemistry-Paraffin-NB100-56148-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: NLRP1/NALP1 Antibody [NB100-56148] - Gene expression & cellular distribution of NLRP1, NLRP3 & Aim2 sensors in the retina. (A) RNAscope analysis of NLRP1 (green dots, yellow arrows) & NLRP3 (white dots, white arrows) transcript abundance in normotensive control (NT control) & experimental (OHT) retinas at 12 h postinjury. (B) Aim2 transcript (red dots) abundance in normotensive control & experimental retinas. Co-staining for the Muller glia marker glutamine synthetase (GlSyn, green) is added to indicate cellular expression of Aim2 in the GCL layer (yellow arrows) & INL (white arrows) in control & 12 h post-OHT retinas. Bar, 25 μm on all panels. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30930743), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56148_rabbit-polyclonal-nlrp1-nalp1-antibody-31020241534381.jpg)
![Immunocytochemistry/ Immunofluorescence: NLRP1/NALP1 Antibody [NB100-56148] - OHT-induced upregulation of Aim2 inflammasome in the retina. (A) NLRP1 antibody labeling (red) in the inner retina; yellow arrows indicate colocalization with RGC neurons (RBPSM+) in the GCL. (B) Immunohistochemistry for Aim2 protein (red) & Muller glia marker (GlSyn, green). Arrows show colocalization with Muller glia; no colocalization is detected with astrocytes (GFAP, white) in both control & 24 h post-OHT retinas. (C) NLRP3 labeling (green) in the inner retina of naïve & control eyes localized only to blood capillaries (white arrowhead) & astrocytes (yellow arrows), but not to RGCs (RBPMS cells). At 24 h post-injury the labeling in large cells in the GCL & INL (white arrows) are observed. (D) The NLRP1and NLRP3 proteins localized to distinct cell types in the GCL of naïve & NT control retinas: NLRP3 (green) is only expressed in blood vessels (white arrowheads). Twenty four hours after OHT, NLRP3 colocalizes with NLRP1 in RGCs (yellow arrows) & to RBPMS- cells (white arrows). Blue arrowheads denote labeling in RGC axons & dendrites. Bar, 25 μm on all panels. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30930743), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56148_rabbit-polyclonal-nlrp1-nalp1-antibody-310202415541962.jpg)
![Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western Blot analysis of Rat brain membrane lysate (native) showing detection of ~261 kDa Cav3.2protein using Mouse Anti-Cav3.2Monoclonal Antibody, Clone N55/10 (NBP1-22444). Block: 2% Skim Milk + 2% BSA in TBST. Primary Antibody: Mouse Anti-Cav3.2Monoclonal Antibody (NBP1-22444) at 1:1000 for 2 hours at RT. Secondary Antibody: Anti-Mouse: HRP at 1:4000. Predicted/Observed Size: ~261 kDa.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Western-Blot-NBP1-22444-img0014.jpg "Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western Blot analysis of Rat brain membrane lysate (native) showing detection of ~261 kDa Cav3.2protein using Mouse Anti-Cav3.2Monoclonal Antibody, Clone N55/10 (NBP1-22444). Block: 2% Skim Milk + 2% BSA in TBST. Primary Antibody: Mouse Anti-Cav3.2Monoclonal Antibody (NBP1-22444) at 1:1000 for 2 hours at RT. Secondary Antibody: Anti-Mouse: HRP at 1:4000. Predicted/Observed Size: ~261 kDa.")
![Immunohistochemistry-Paraffin: Cav3.2 Antibody (S55/10) [NBP1-22444] - Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Immunohistochemistry-Paraffin-NBP1-22444-img0011.jpg)
![Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western Blot using rat brain membranes and NBP1-22444 to target Cav3.2 channels visible at about 260 kDa.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Western-Blot-NBP1-22444-img0012.jpg)
![Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western blot analysis of CaV3.2 proteins in mesenteric arteries of young versus old mice. (h), quantification of Western blot results. Mesenteric arteries were taken from 9 mice in each group. *, p < .05. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/abs/10.1111/acel.13134) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Western-Blot-NBP1-22444-img0013.jpg)
![Immunohistochemistry-Frozen: Cav3.2 Antibody (S55/10) [NBP1-22444] - Tissue: frozen brain section. Species: human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 uL for 5 minutes at RT.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Immunohistochemistry-Frozen-NBP1-22444-img0010.jpg)
![Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Age attenuates the role of CaV3.2 channels in Ca2+ spark generation & decreases CaV3.2 protein expression in VSMC. (a), Ca2+ fluorescence images of a Fluo‐4‐AM–loaded VSMC from a young mouse & time course of Ca2+ fluorescence changes in the cellular ROI (upper panel). Cell boundary is marked with dashed line. (b), same as (a) but in the presence of Ni2+ (50 µM). (c), same as (a) but in a VSMC from an old mouse. (d), same as (c) but in the presence of Ni2+ (50 µM). (e, f), summary of the results. Ca2+ spark frequency (e) & fraction of cells producing Ca2+ sparks (f) in VSMCs from young mice (n = 102), in VSMCs from young mice cells incubated with Ni2+ (n = 85), in VSMCs from aged mice (n = 129), & in VSMCs from aged mice cells incubated with Ni2+ (n = 127). Cells were isolated from 4 mice in each group; 25–40 cells were recorded & analyzed from each mouse. VSMC, vascular smooth muscle cell. (g), Western blot analysis of CaV3.2 proteins in mesenteric arteries of young versus old mice. (h), quantification of Western blot results. Mesenteric arteries were taken from 9 mice in each group. *, p < .05. n.s., not significant Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32187825), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-22444_mouse-monoclonal-cav3-2-antibody-s55-10-31020241617145.jpg)
![Western Blot: FBXO11 Antibody [NB100-59826] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FBXO11 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human FBXO11 Polyclonal Antibody (Catalog # NB100-59826) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FBXO11 at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FBXO11-Antibody-Knockout-Validated-NB100-59826-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FBXO11 Antibody [NB100-59826] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FBXO11 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human FBXO11 Polyclonal Antibody (Catalog # NB100-59826) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FBXO11 at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: FBXO11 Antibody [NB100-59826] - Detection of Human FBXO11 on HeLa whole cell lysate using NB100-59826. FBXO11 was also immunoprecipitated by rabbit anti-FBXO11 antibody NB100-59825.](http://images.novusbio.com/fullsize/FBXO11-Antibody-Western-Blot-NB100-59826-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: FBXO11 Antibody [NB100-59826] - (A) Breast cancer cell line MDA-MB231-LM2 with/without FBXO11 knockdown. (B) 4T1 xenograft tumors with/without FBXO11 knockdown. Image from verified customer review.](http://images.novusbio.com/fullsize/FBXO11-Antibody-Western-Blot-NB100-59826-img0006.jpg)
![Western Blot: FBXO11 Antibody [NB100-59826] - Analysis of FBXO11 in Raji whole cell lysate (40 ug/lane) using anti-FBXO11 antibody (Cat. # NB100-59826). Image from verified customer review.](http://images.novusbio.com/fullsize/FBXO11-Antibody-Western-Blot-NB100-59826-img0002.jpg)
![Simple Western: FBXO11 Antibody [NB100-59826] - Simple Western lane view shows a specific band for FBXO11 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FBXO11-Antibody-Simple-Western-NB100-59826-img0003.jpg)
![Western Blot: Aurora B Antibody - BSA Free [NB100-294] - Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Aurora B antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. Negative Control](http://images.novusbio.com/fullsize/Aurora-B-Antibody---BSA-Free-Western-Blot-NB100-294-img0002.jpg "Western Blot: Aurora B Antibody - BSA Free [NB100-294] - Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Aurora B antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. Negative Control")
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294] - HeLa human cervix epitheloid carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by cold methanol. Fixed cells were stained with 1:50 Anti-Aurora B antibody produced in rabbit. The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat.](http://images.novusbio.com/fullsize/Aurora-B-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-294-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (Green) P0 concentration was 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). Red is actin. Blue is DAPI nuclear counterstain.](http://images.novusbio.com/fullsize/Myelin-Protein-Zero-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1607-img0001.jpg "Immunocytochemistry/Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (Green) P0 concentration was 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). Red is actin. Blue is DAPI nuclear counterstain.")
![Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607] - In the image (a lower power tissue section through an adult sciatic nerve), Myelin Protein Zero (brown staining) can be seen in all of the myelinating Schwann cells.](http://images.novusbio.com/fullsize/Myelin-Protein-Zero-Antibody-Immunohistochemistry-NB100-1607-img0002.jpg)
![Western Blot: O-GlcNAc Antibody (HGAC85) [NB300-614] - alpha-DG-Fc was transiently transfected with or without AGO61 into COS1 cells. alpha-DG-Fc recombinant proteins were collected from cell lysates and culture media using protein A resin and analyzed for laminin overlay and Western blot using anti-Fc and anti-O-GlcNAc antibodies. Image collected and cropped by CiteAb from the following publication (nature.com/articles/srep03288), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-HGAC85-Western-Blot-NB300-614-img0003.jpg "Western Blot: O-GlcNAc Antibody (HGAC85) [NB300-614] - alpha-DG-Fc was transiently transfected with or without AGO61 into COS1 cells. alpha-DG-Fc recombinant proteins were collected from cell lysates and culture media using protein A resin and analyzed for laminin overlay and Western blot using anti-Fc and anti-O-GlcNAc antibodies. Image collected and cropped by CiteAb from the following publication (nature.com/articles/srep03288), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: O-GlcNAc Antibody (HGAC85) [NB300-614] - IHC staining of O-GlcNAc in mouse colon using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-HGAC85-Immunohistochemistry-Paraffin-NB300-614-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: O-GlcNAc Antibody (HGAC85) [NB300-614] - Western blot analysis of O-GlcNAc expression in 1) HeLa, 2) NTERA-2, 3) PC-12 and 4) COS-7 cell lysates using untreated antibody on the left side and antibody preincubated with 50 uM GlcNAc (see Notes).](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-HGAC85-Western-Blot-NB300-614-img0002.jpg)
![Western Blot: O-GlcNAc Antibody (HGAC85) [NB300-614] - AGO61 modifies GlcNAc residues at specific sites on alpha -DG.(a) alpha -DG-Fc was transiently transfected with or without AGO61 into COS1 cells. alpha -DG-Fc recombinant proteins were collected from cell lysates & culture media using protein A resin & analyzed for laminin overlay & Western blot using anti-Fc & anti-O-GlcNAc antibodies. (b) alpha -DG373-HALO & its mutant T317A/T319A were transiently transfected with or without LARGE-myc into COS7 cells. HALO-fused proteins were collected from medium using HALO resin followed by digestion with TEV protease & then analyzed by laminin overlay. Cell lysates were analyzed for the expression of HALO-fused proteins & LARGE-myc by Western blot using anti-HALO & anti-myc antibodies. The full-length blots with anti-HALO & anti-myc antibodies are presented in Supplementary Figs. S7f & S7g, respectively. (c) alpha -DG373-HALO & its mutant T317A/T319A were transiently transfected with or without AOG61 into COS7 cells. HALO-fused proteins were collected from the cell lysates using HALO resin followed by digestion with TEV protease & then analyzed by Western blot using an anti-O-GlcNAc antibody (CTD110.6). The cell lysates were analyzed for the expression of HALO-fused proteins & AGO61 by Western blot using anti-HALO & anti-AGO61 antibodies. The full-length blots with anti-HALO & anti-AGO61 antibodies are presented in Supplementary Figs. S7h & S7i, respectively. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep03288), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-614_mouse-monoclonal-o-glcnac-antibody-hgac85-31020241618919.jpg)
![Western Blot: NKX3.1 Antibody (0361) [NB100-1828] - Analysis of NKX3.1 in HeLa cell lysate using anti-NKX3.1 antibody. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NKX3-1-Antibody-0361-Western-Blot-NB100-1828-img0006.jpg "Western Blot: NKX3.1 Antibody (0361) [NB100-1828] - Analysis of NKX3.1 in HeLa cell lysate using anti-NKX3.1 antibody. WB image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) [NB100-1828] - Staining of human chondrosarcoma tissue with NKX3.1 monoclonal antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NKX3-1-Antibody-0361-Immunohistochemistry-Paraffin-NB100-1828-img0008.jpg)
![Western Blot: Nkx3.1 Antibody (0361) [NB100-1828] - Detection of NKX3.1 in mouse testis lysate using NB 100-1828. ECL detection 1 minute.](http://images.novusbio.com/fullsize/Nkx3-1-Antibody-0361-Western-Blot-NB100-1828-img0003.jpg)
![Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) [NB100-1828] - NKX3.1 was detected in immersion fixed paraffin-embedded sections of mouse testis using Mouse Anti-Mouse NKX3.1 (0361) Monoclonal Antibody (Catalog # NB100-1828) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei in sperm cells.](http://images.novusbio.com/fullsize/NKX3-1-Antibody-0361-Immunohistochemistry-Paraffin-NB100-1828-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: NKX3.1 Antibody (0361) [NB100-1828] - DWB analysis of NKX3.1 expression in the indicated cells transfected with Ri-Vector (V-Ri) or shRNAs (Ri#1/2) against NKX3.1. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC6895605/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NKX3-1-Antibody-0361-Knockdown-Validated-NB100-1828-img0009.jpg)
![Western Blot: NKX3.1 Antibody (0361) - BSA Free [NB100-1828] - SALL2 transcriptionally upregulates ESR1 in breast cancerARNA‐seq analysis of ESR1 mRNA levels in 9 paired pre‐tamoxifen‐treated primary breast cancer tissues & relapsed tamoxifen‐resistant breast cancer tissues.BqRT–PCR analysis of ESR1 expression in 9 paired pre‐tamoxifen‐treated primary breast cancer tissues & relapsed tamoxifen‐resistant breast cancer tissues. GAPDH was used as an internal control.C, DWB analysis of SALL2 (A) & NKX3‐1 (B) expression in the indicated cells transfected with Ri‐Vector (V‐Ri) or shRNAs (Ri#1/2) against SALL2 or NKX3‐1. alpha ‐Tubulin was used as the loading control.E, FqRT–PCR analysis of ESR1 expression in the indicated cells transfected with Ri‐Vector or shRNAs (Ri#1/2) against SALL2 or NKX3‐1.Data information: In (A), P‐values were determined by two‐tailed paired Student's t‐test. In (B), data are presented as mean ± SD, & P‐values were determined by two‐tailed unpaired Student's t‐test, n = 3. In (E & F), data are presented as mean ± SD, & P‐values were determined by one‐way ANOVA test, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., no significance. Exact P‐values are specified in Appendix Table S10.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31657150), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1828_mouse-monoclonal-nkx3-1-antibody-0361-310202415525249.jpg)
