Biological Strategies: Western Blot: O-GlcNAc Antibody (HGAC85) [NB300-614] - Western blot analysis of O-GlcNAc expression in 1) HeLa, 2) NTERA-2, 3) PC-12 and 4) COS-7 cell lysates using untreated antibody on ...read more
Immunohistochemistry-Paraffin: O-GlcNAc Antibody (HGAC85) [NB300-614] - IHC staining of O-GlcNAc in mouse colon using DAB with hematoxylin counterstain.
This O-GlcNAc Antibody (HGAC85) is useful for Western blot, Chromatin Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin and Immunoprecipitation. ChIP assay reported in literature (PMID: 20368426). ICC and IP reported (Hamiel CR, et al). IHC reported (see Zhang X, et al). In Western blot, this antibody detects several proteins representing O-GlcNAc glycoproteins. Immunofluorescence staining of O-GlcNAc in cells results in labeling of the nuclear envelope and pores, nucleolus, and cytoplasm. This staining pattern is consistent with other methods of detecting O-GlcNAc moieties. DO NOT USE WITH DILUENTS CONAINING GLYCOSYLATED PROTEINS.
All Species. Human reactivity reported in scientific literature (PMID: 28973823).
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.1% Sodium Azide
Specificity to O-GlcNAc was verified in Western blot (see Images) by incubation of NB300-614 with 50 mM N-acetylglucosamine (Sigma Cat. No. A3286).
Alternate Names for O-GlcNAc Antibody (HGAC85)
O-GlcNAc (O-linked N-acetylglucosamine) is one of the most abundant posttranslational modifications on nuclear and cytoplasmic proteins. Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-GlcNAc residues attached to serine or threonine residues. This modification occurs via the O-GlcNAc transferase (OGT). There appears to be a competitive modification at these residues. Either the residues are glycosylated or they are phosphorylated. Because of the significance of phosphorylation in cancer research, such competition can be of major interest. O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may also obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling. Recent research has revealed that the O-GlcNAcylation of the EGF repeats of Notch are modified via a novel O-GlcNAc transferase, EOGT1 (PMID: 22310717).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for O-GlcNAc Antibody (NB300-614)(10)
We have publications tested in 3 confirmed species: Human, Rat, Primate.
We have publications tested in 2 applications: ICC/IF, WB.
O-GlcNAc, Glucose Deprivation and Cancer O-linked beta-N-acetylglucosamine (O-GlcNAc) is a sugar attachment to serine or threonine hydroxyl moieties on nuclear and cytoplasmic proteins. O-GlcNAc modified proteins are generally either cytoplasmic or nuclear proteins, and unlike asparagine-lin... Read full blog post.
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