HPV16 L1 Antibody (CamVir 1) - Azide and BSA Free

• Reactivity: V-Vi
• Applications: WB, ELISA, ICC/IF, IHC, IP, Mycoplasma
• Clone: CamVir 1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

HPV16 L1 Antibody (CamVir 1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HPV16 L1 Antibody (CamVir 1) - Azide and BSA Free (NB100-2732) is a monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A beta galactosidase-L1 fusion protein purified by PAGE.
• Epitope: C terminus of HPV16 L1
• Localization: Virion. Host nucleus.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 24231739)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:100-1:2000
• Application Notes: This HPV16 L1 (CamVir 1) antibody reacts with a 56 kDa protein in cells infected with L1-vaccinia virus, the protein being present in a predominantly nuclear location. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human Papillomavirus (HPV)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HPV16 L1 Antibody (CamVir 1) - Azide and BSA Free

• HPV16 capsid protein
• HPV-16 capsid
• HPV16 L1
• HPV16 major capsid protein L1
• HPV-16
• Human papillomavirus type 16 L1
• Human papillomavirus type 16 major capsid protein L1
• Major capsid protein L1
• Major capsid protein

Background

HPV16 L1, also known as HPV-16, Major capsid protein L1, and Major Capsid Protein is a papillomavirus. Papillomaviruses are small nonenveloped tumorigenic DNA viruses that infect the basal cells of the epithelium. Papillomavirions contain two proteins, L1 and L2, which encapsulate a closed, circular, double-stranded DNA. The major capsid protein L1 specifically binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. Thus, the location of HPV16 L1 is largely nuclear. Furthermore, stable interactions have been observed between L1 and the minor capsid protein L2, which is necessary for viral genome encapsulation. Virtually all cervical cancers are caused by human papilloma infections, with types 16 and 18 being responsible for about 70 percent of all cases. Currently, B-cell epitopes from type 16-L1 derived peptides are being investigated in order to gain a better understanding of immune responses to this virus (PMID: 22979950).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.