HPV16 L1 Antibody (CamVir 1)

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Product Details

Summary
Reactivity ViSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
CamVir 1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

HPV16 L1 Antibody (CamVir 1) Summary

Immunogen
A beta galactosidase-L1 fusion protein purified by PAGE.
Epitope
C terminus of HPV16 L1
Localization
Virion. Host nucleus.
Specificity
Cross reacts with HPV37. Reacts very strongly with biopsy specimins containing HPV16 or HPV33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV6 or HPV11.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This HPV16 L1 (CamVir 1) antibody is useful for Immunoprecipitation, Western Blot, Immunohistochemistry on paraffin-embedded sections and ELISA. This antibody reacts with a 56 kDa protein in cells infected with L1-vaccinia virus, the protein being present in a predominantly nuclear location. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24231739)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-2732 in the following applications:

  • 2 publications
  • 2 publications
  • WB
    10 publications

Reactivity Notes

Human Papillomavirus (HPV)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for HPV16 L1 Antibody (CamVir 1)

  • HPV16 capsid protein
  • HPV-16 capsid
  • HPV16 L1
  • HPV16 major capsid protein L1
  • HPV-16
  • Human papillomavirus type 16 L1
  • Human papillomavirus type 16 major capsid protein L1
  • Major capsid protein L1
  • Major capsid protein

Background

HPV16 L1, also known as HPV-16, Major capsid protein L1, and Major Capsid Protein is a papillomavirus. Papillomaviruses are small nonenveloped tumorigenic DNA viruses that infect the basal cells of the epithelium. Papillomavirions contain two proteins, L1 and L2, which encapsulate a closed, circular, double-stranded DNA. The major capsid protein L1 specifically binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. Thus, the location of HPV16 L1 is largely nuclear. Furthermore, stable interactions have been observed between L1 and the minor capsid protein L2, which is necessary for viral genome encapsulation. Virtually all cervical cancers are caused by human papilloma infections, with types 16 and 18 being responsible for about 70 percent of all cases. Currently, B-cell epitopes from type 16-L1 derived peptides are being investigated in order to gain a better understanding of immune responses to this virus (PMID: 22979950).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HPV16 L1 Antibody (NB100-2732)(13)

We have publications tested in 1 confirmed species: Virus.

We have publications tested in 3 applications: ELISA, IHC-P, WB.


Filter By Application
ELISA
(2)
IHC-P
(2)
WB
(10)
All Applications
Filter By Species
Virus
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-2732 Applications Species
Palaniyandi M, Kato T, Park EY. Expression of human papillomavirus 6b L1 protein in silkworm larvae and enhanced green fluorescent protein displaying on its virus-like particles. SpringerPlus. 2012 [PMID: 23961359] (WB, Virus) WB Virus
Orozco J, Carter JJ, Koutsky LA, Galloway DA. Humoral immune response recognizes a complex set of epitopes on human papillomavirus type 6 l1 capsomers J Virol. 2005 Aug [PMID: 16014913] (WB) WB
Carter JJ, Wipf GC, Benki SF et al. Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. J Virol. 2003 Nov [PMID: 14557648] (WB, ELISA) WB, ELISA
Benyacoub J, Hopkins S, Potts A et al. The nature of the attenuation of Salmonella typhimurium strains expressing human papillomavirus type 16 virus-like particles determines the systemic and mucosal antibody responses in nasally immunized mice. Infect Immun. 1999 Jul [PMID: 10377159] (WB) WB
Caparros-Wanderley W, Savage N, Hill-Perkins M et al. Intratype sequence variation among clinical isolates of the human papillomavirus type 6 L1 ORF: clustering of mutations and identification of a frequent amino acid sequence variant. J Gen Virol. 1999 Apr [PMID: 10211973] (WB) WB
Nardelli-Haefliger D, Roden RB, Benyacoub J et al. Human papillomavirus type 16 virus-like particles expressed in attenuated Salmonella typhimurium elicit mucosal and systemic neutralizing antibodies in mice. Infect Immun. 1997 Aug [PMID: 9234794] (WB) WB
Sasaqawa T, Inoue M, Lehtinen M et al. Serological responses to human papillomavirus type 6 and 16 virus-like particles in patients with cervical neoplastic lesions. Clin Diagn Lab Immunol. 1996 Jul [PMID: 8807204] (ELISA) ELISA
Park DS, Selvey LA, Kelsall SR et al. Human papillomavirus type 16 E6, E7 and L1 and type 18 E7 proteins produced by recombinant baculoviruses. J Virol Methods. 1993 Dec 31 [PMID: 8106603]
Stering J, Stanley M, Gatward G et al. Production of human papillomavirus type 16 virions in a keratinocyte cell line. J Virol. 1990 Dec [PMID: 2173795] (IHC-P) IHC-P
Zhou J, Crawford L, McLean L et al. Increased antibody responses to human papillomavirus type 16 L1 protein expressed by recombinant vaccinia virus lacking serine protease inhibitor genes. J Gen Virol. 1990 Sep [PMID: 2170578] (WB) WB
Show All 13 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

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