Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-94712SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96878SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1983-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13249-0.2mg | |||
| BSA Free | NB120-13249-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2339-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-316SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB484-SP | |||
| Carrier Free | MAB484-500 | |||
| Carrier Free | FAB484V-100UG | |||
| Carrier Free | FAB484U-100UG | |||
| Carrier Free | FAB484T-100UG | |||
| Carrier Free | FAB484S-100UG | |||
| Carrier Free | FAB484R-100UG | |||
| Carrier Free | FAB484N-100UG | |||
| Carrier Free | FAB484G-100UG | |||
![Western Blot: Senataxin Antibody [NBP1-94712] - Input: HeLa whole cell lysate. Beads without antibody IP control. IP: IP from HeLa lysate.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Western-Blot-NBP1-94712-img0002.jpg "Western Blot: Senataxin Antibody [NBP1-94712] - Input: HeLa whole cell lysate. Beads without antibody IP control. IP: IP from HeLa lysate.")
![Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP1-94712] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Senataxin at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-94712-img0010.jpg)
![Immunohistochemistry-Paraffin: Senataxin Antibody [NBP1-94712] - Analysis of a FFPE tissue section of human heart (transverse section) using 1:200 dilution of lot A2 of Senataxin antibody. The antibody generated a very strong staining in the cytoplasm and the nuclei of the muscle cells. No signal was found in the perimysium and endomycium area (connective tissue) of the section.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunohistochemistry-Paraffin-NBP1-94712-img0007.jpg)
![Flow Cytometry: Senataxin Antibody [NBP1-94712] - An intracellular stain was performed on Jurkat cells and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, .](http://images.novusbio.com/fullsize/Senataxin-Antibody-Flow-Cytometry-NBP1-94712-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP1-94712] - Senataxin antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-94712-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP1-94712] - Senataxin immunofluorescence in fibroblasts. Image from verified customer review.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-94712-img0004.jpg)
![Immunohistochemistry-Paraffin: Senataxin Antibody [NBP1-94712] - Analysis of a FFPE tissue section of human heart (verticle section) using 1:200 dilution of lot A2 of Senataxin antibody. The antibody generated a very strong staining in the cytoplasm and the nuclei of the muscle cells. No signal was found in the perimysium and endomycium (connective tissue) of the section.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunohistochemistry-Paraffin-NBP1-94712-img0006.jpg)
![Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody. The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.](http://images.novusbio.com/fullsize/CD44-Antibody-Western-Blot-NBP1-31488-img0031.jpg "<b>Biological Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody. The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.")
![<b>Biological Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody. The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.](http://images.novusbio.com/fullsize/CD44-Antibody-Western-Blot-NBP1-31488-img0033.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP1-31488] - Analysis of methanol-fixed HeLa, using CD44 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31488-img0016.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human cervical carcinoma. CD44 stained by CD44 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-31488-img0036.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human endometrial carcinoma. CD44 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-31488-img0026.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human pancreatic tumor, using CD44 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-31488-img0035.jpg)
![Immunocytochemistry/ Immunofluorescence: CD44 Antibody [NBP1-31488] - CD44 antibody detects CD44 protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: CD44 stained by CD44 antibody (NBP1-31488) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31488_rabbit-polyclonal-cd44-antibody-91020242091445.jpg)
![Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody (NBP1-31488) diluted at 1:45000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31488_rabbit-polyclonal-cd44-antibody-910202420104431.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Non-transfected (–) and transfected (+) Wild-type (WT) and G CD44 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31488_rabbit-polyclonal-cd44-antibody-1610202419405756.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-31488_rabbit-polyclonal-cd44-antibody-1610202419414564.jpg)
![Immunocytochemistry/Immunofluorescence: DNA/RNA Damage Antibody (15A3) [NB110-96878] - visualized on a retinal injury](http://images.novusbio.com/fullsize/DNA-RNA-Damage-Antibody-15A3-Immunocytochemistry-Immunofluorescence-NB110-96878-img0004.jpg "Immunocytochemistry/Immunofluorescence: DNA/RNA Damage Antibody (15A3) [NB110-96878] - visualized on a retinal injury")
![Immunohistochemistry: DNA/RNA Damage Antibody (15A3) [NB110-96878] - Immunohistochemistry analysis using Mouse Anti-DNA/RNA DamageMonoclonal Antibody, Clone 15A3 (NB110-96878). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-DNA/RNA DamageMonoclonal Antibody (NB110-96878) at 1:1000000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x. With anti-microbial.](http://images.novusbio.com/fullsize/DNA-RNA-Damage-Antibody-15A3-Immunohistochemistry-NB110-96878-img0013.jpg)
![DNA/RNA Damage Antibody (15A3) [NB110-96878] - Immunohistochemistry analysis using Mouse Anti-DNA/RNA DamageMonoclonal Antibody, Clone 15A3 (NB110-96878). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-DNA/RNA DamageMonoclonal Antibody (NB110-96878) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/DNA-RNA-Damage-Antibody-15A3-NB110-96878-img0014.jpg)
![Western Blot: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Western Blot analysis of Rat brain membrane lysate showing detection of CaM Kinase II alpha protein using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:1000.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Western-Blot-NB100-1983-img0015.jpg "Western Blot: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Western Blot analysis of Rat brain membrane lysate showing detection of CaM Kinase II alpha protein using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunocytochemistry-Immunofluorescence-NB100-1983-img0018.jpg)
![Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunohistochemistry analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunohistochemistry-NB100-1983-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunocytochemistry-Immunofluorescence-NB100-1983-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: dissociated hippocampal neurons. Species: Mouse. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunocytochemistry-Immunofluorescence-NB100-1983-img0014.jpg)
![Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunohistochemistry analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunohistochemistry-NB100-1983-img0016.jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-49956] - p53 repression of autophagy is PINK1-dependent. LC3-2/LC3-1 (a,b, N = 12), p62/SQSTM1 (a,c, N = 12), TIM23 (a,d, N = 12), TOM20 (a,e, N = 12) and HSP60 (a,f, N = 12) protein levels were analyzed in control (PINK+/+) or Pink1-deficient (PINK-/-) HAP cells treated with vehicle (Ct), pifithrin-alpha (PFT, 30 uM, 4 h) or CCCP (CP, 10 uM, 6 h) as described in the Methods. Bars represent the means +/- SEM of 4 independent experiments performed in triplicates and are expressed as percent of control HAP1 (HAP+/+, Ct) cells. Actin expression is provided as a representative gel loading control. Statistical analyses were performed with GraphPad Prism software by using unpaired Student's t-test. Significant differences are: ns not significant, *p < 0.05, ** p < 0.01 and ***p < 0.001. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41418-017-0016-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49956-img0006.jpg "Western Blot: p62/SQSTM1 Antibody [NBP1-49956] - p53 repression of autophagy is PINK1-dependent. LC3-2/LC3-1 (a,b, N = 12), p62/SQSTM1 (a,c, N = 12), TIM23 (a,d, N = 12), TOM20 (a,e, N = 12) and HSP60 (a,f, N = 12) protein levels were analyzed in control (PINK+/+) or Pink1-deficient (PINK-/-) HAP cells treated with vehicle (Ct), pifithrin-alpha (PFT, 30 uM, 4 h) or CCCP (CP, 10 uM, 6 h) as described in the Methods. Bars represent the means +/- SEM of 4 independent experiments performed in triplicates and are expressed as percent of control HAP1 (HAP+/+, Ct) cells. Actin expression is provided as a representative gel loading control. Statistical analyses were performed with GraphPad Prism software by using unpaired Student's t-test. Significant differences are: ns not significant, *p < 0.05, ** p < 0.01 and ***p < 0.001. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41418-017-0016-0), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: p62/SQSTM1 Antibody [NBP1-49956] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Sequestosome-1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Immunohistochemistry-Paraffin-NBP1-49956-img0004.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: p62/SQSTM1 Antibody [NBP1-49956] - Detection of Human and Mouse Sequestosome-1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies which recognize upstream epitopes. For blotting immunoprecipitated Sequestosome-1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49956-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-49956] - Evaluation of mitophagy control by p53 in mice brain. p62/SQSTM1 (N = 9) protein levels were analyzed in control (p53+/+) or TP53-deficient (p53-/-) knockout mice brains as described in the Methods. Bars represent the means +/- SEM of 3 independent experiments performed in triplicate and are expressed as percent of control (p53+/+) cells. Actin expression is provided as a representative gel loading control. Statistical analyses were performed with GraphPad Prism software by using unpaired Student's t-test. Significant differences are: ** p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41418-017-0016-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49956-img0005.jpg)
![Western Blot: AMPK alpha 1 Antibody [NB100-239] - Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Western-Blot-NB100-239-img0006.jpg "Western Blot: AMPK alpha 1 Antibody [NB100-239] - Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry: AMPK alpha 1 Antibody [NB100-239] - Detection of human AMPK alpha 1 by immunohistochemistry. Sample: FFPE section of human smooth muscle. Antibody: Affinity purified rabbit anti-AMPK alpha 1 (NB100-239). Detection: DAB](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Immunohistochemistry-NB100-239-img0005.jpg)
![Immunoprecipitation: AMPK alpha 1 Antibody [NB100-239] - Detection of human AMPK alpha 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 (lot 2) used for IP at 3 ug per reaction. AMPK alpha 1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated AMPK alpha 1, NB100-239 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Immunoprecipitation-NB100-239-img0007.jpg)
![Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat brain tissue lysate.](http://images.novusbio.com/fullsize/Bassoon-Antibody-SAP7F407-Western-Blot-NB120-13249-img0001.jpg "Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat brain tissue lysate.")
![Immunocytochemistry/Immunofluorescence: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat Hippocampal Neuron, image courtesy of customer.](http://images.novusbio.com/fullsize/Bassoon-Antibody-SAP7F407-Immunocytochemistry-Immunofluorescence-NB120-13249-img0003.jpg)
![Immunohistochemistry: Bassoon Antibody (SAP7F407) [NB120-13249] - Bassoon localization in the rat brain cerebellar molecular layer using Bassoon Monoclonal Antibody.](http://images.novusbio.com/fullsize/Bassoon-Antibody-SAP7F407-Immunohistochemistry-NB120-13249-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (MEM-263) [NB500-481] - analysis of CD44 in 4% paraformaldehyde fixed Caov-3 cells (adherent human ovarian cancer cells) using anti-Cd44 antibody. The primary antibody was used at a dilution of 1:1000 in 2% goat serum in PBS and incubated for 2 hours at room temperature. Image from verified customer review.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Immunocytochemistry-Immunofluorescence-NB500-481-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD44 Antibody (MEM-263) [NB500-481] - analysis of CD44 in 4% paraformaldehyde fixed Caov-3 cells (adherent human ovarian cancer cells) using anti-Cd44 antibody. The primary antibody was used at a dilution of 1:1000 in 2% goat serum in PBS and incubated for 2 hours at room temperature. Image from verified customer review.")
![CD44 Antibody (MEM-263) [NB500-481] - Analysis of CD44 in HeLa cells using anti-CD44 (MEM-263) purified; non-reducing conditions.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-NB500-481-img0009.jpg)
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - A surface stain was performed on hPBMCs with CD44 (MEM-263) antibody NB500-481AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0003.jpg)
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) APC conjugate.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0007.jpg)
![Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Expression of CD44 and CK18 in multiple clones of ovarian cancer cells. CD44+ cells also express CK18 as determined by western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jo/2011/620523/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Western-Blot-NB500-481-img0008.jpg)
![Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Fig. 1. Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Western-Blot-NB500-481-img0002.jpg)
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Analysis of human peripheral blood (lymphocyte gate) using anti-CD44 (MEM-263) PE conjugate.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0004.jpg)
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) FITC conjugate.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0005.jpg)
![Simple Western: FANCD2 Antibody (FI-17) [NB100-316] - Image shows a specific band for FANCD2 in 1 mg/mL of HeLa cell lysate using FANCD2 Antibody (FI-17). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FANCD2-Antibody-FI-17-Simple-Western-NB100-316-img0005.jpg "Simple Western: FANCD2 Antibody (FI-17) [NB100-316] - Image shows a specific band for FANCD2 in 1 mg/mL of HeLa cell lysate using FANCD2 Antibody (FI-17). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - MCF7 cell line (lane 1, molecular weight: 164.1 KDa) and HeLa whole cell extract (lane 2) using FANCD2 Antibody (FI-17).](http://images.novusbio.com/fullsize/FANCD2-Antibody-FI-17-Western-Blot-NB100-316-img0003.jpg)
![Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - Expression of FANCD2 (Molecular weight: 164.1 KDa) in different cell lines. Proliferating cells were treated with 10-Gy IR. 50 ug protein was loaded each lane. FANCD2 Antibody (FI-17) at 1:1000.](http://images.novusbio.com/fullsize/FANCD2-Antibody-FI-17-Western-Blot-NB100-316-img0004.jpg)
