Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97921SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2066-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1924SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1676-SP | |||
| Carrier Free | MAB1676-500 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB1408U-100UG | |||
| Alexa Fluor 405 | FAB1408V-100UG | |||
| Alexa Fluor 488 | FAB1408G-100UG | |||
| Alexa Fluor 532 | FAB1408X-100UG | |||
| Alexa Fluor 594 | FAB1408T-100UG | |||
| Alexa Fluor 647 | FAB1408R-100UG | |||
| Alexa Fluor 700 | FAB1408N-100UG | |||
| Alexa Fluor 750 | FAB1408S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1408-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1344-SP | |||
| Carrier Free | MAB1344-500 | |||
![Western Blot: UVRAG Antibody [NBP1-18885] - Beclin 1 is acetylated at lysines 430 and 437. TSA and NAM increase the binding of Beclin 1 to Rubicon. Immunoprecipitation of indicated Beclin 1-binding partners with ectopically expressed Flag-Beclin 1 in HEK293T cells treated with TSA and NAM. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms8215), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/UVRAG-Antibody-Western-Blot-NBP1-18885-img0005.jpg "Western Blot: UVRAG Antibody [NBP1-18885] - Beclin 1 is acetylated at lysines 430 and 437. TSA and NAM increase the binding of Beclin 1 to Rubicon. Immunoprecipitation of indicated Beclin 1-binding partners with ectopically expressed Flag-Beclin 1 in HEK293T cells treated with TSA and NAM. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms8215), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: UVRAG Antibody [NBP1-18885] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- UVRAG used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/UVRAG-Antibody-Immunohistochemistry-Paraffin-NBP1-18885-img0004.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: UVRAG Antibody [NBP1-18885] - Whole cell lysate from HeLa and 293T cells. UVRAG was also immunoprecipitated by rabbit anti-UVRAG antibody NBP1-18884.](http://images.novusbio.com/fullsize/UVRAG-Antibody-Western-Blot-NBP1-18885-img0003.jpg)
![Western Blot: UVRAG Antibody [NBP1-18885] - Beclin 1 is acetylated at lysines 430 and 437. Mutations of K430 and K437 decrease the binding of Beclin 1 and Rubicon. Immunoprecipitation of indicated proteins with Beclin 1 in HEK293T cells transfected with indicated Beclin 1 constructs. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms8215), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/UVRAG-Antibody-Western-Blot-NBP1-18885-img0006.jpg)
![Western Blot: SM047 Antibody (SM047) [NBP1-97921] - SM047 immunoprecipitates immunoblotted with the SM047 antibody. Lane 1) UWOV1 lysate, Lane 2) ovarian cancer patients serum and Lane 3) pooled normal serum.](http://images.novusbio.com/fullsize/SM047-Antibody-SM047-Western-Blot-NBP1-97921-img0003.jpg "Western Blot: SM047 Antibody (SM047) [NBP1-97921] - SM047 immunoprecipitates immunoblotted with the SM047 antibody. Lane 1) UWOV1 lysate, Lane 2) ovarian cancer patients serum and Lane 3) pooled normal serum.")
![Immunocytochemistry/Immunofluorescence: SM047 Antibody (SM047) [NBP1-97921] - Staining of a cell from an ovarian cancer cell line, labeled with SMO47 antibody. The green fluorescence shows where the antibody has bound to the glycocalyx of the cell.](http://images.novusbio.com/fullsize/SM047-Antibody-SM047-Immunocytochemistry-Immunofluorescence-NBP1-97921-img0005.jpg)
![Immunohistochemistry: SM047 Antibody (SM047) [NBP1-97921] - Immunohistochemical analysis of SM047 in human ovarian cancer using NBP1-97921. Trypsin antigen retrieval was preformed (0.1%, 37C 10 min).](http://images.novusbio.com/fullsize/SM047-Antibody-SM047-Immunohistochemistry-NBP1-97921-img0004.jpg)
![Western Blot: AU1 Epitope Tag Antibody [NB600-452] - Western Blot: AU1 Epitope Tag Antibody [HRP] [NB600-457] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.2 ug/ml (1:5,000). Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/AU1-Epitope-Tag-Antibody-Western-Blot-NB600-452-img0006.jpg "Western Blot: AU1 Epitope Tag Antibody [NB600-452] - Western Blot: AU1 Epitope Tag Antibody [HRP] [NB600-457] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.2 ug/ml (1:5,000). Image using the HRP form of this antibody.")
![Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Lane M: Super Signal Molecular Weight Marker.Lane 1: HeLa WCL. Load: 30 ug lysate.Primary antibody: ROBO1 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 181 kDa, 181 kDa for ROBO1. Other band(s): lower bands not identified.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0015.jpg "Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Lane M: Super Signal Molecular Weight Marker.Lane 1: HeLa WCL. Load: 30 ug lysate.Primary antibody: ROBO1 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 181 kDa, 181 kDa for ROBO1. Other band(s): lower bands not identified.")
![Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NB600-1254] - Staining of Robo1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1254-img0005.jpg)
![Immunohistochemistry: ROBO1 Antibody [NB600-1254] - Affinity Purified ROBO1 antibody was used at a concentration of 5 ug/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunohistochemistry-NB600-1254-img0016.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Biotinylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0012.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western blot using Affinity Purified ROBO1 antibody shows detection of a band at ~181 kDa corresponding to ROBO1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0013.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: MCF-7 Lysate. Lane 4: Jurkat Lysate. Lane 5: A431 Lysate. Lane 6: A549 Lysate. Lane 7: LNCap Lysate. Lane 8: MOLT-4 Lysate. Lane 9: Ramos Lysate. Lane 10: Raji Lysate. Lane 11: A-172 Lysate. Lane 12: NIH/3T3 Lysate. Load: 35 ug per lane. Primary antibody: ROBO1 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted/Observed size: 181kDa for ROBO1.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0014.jpg)
![Immunohistochemistry: ROBO1 Antibody [NB600-1254] - 1/50 staining mouse lung tissue sections (adult, frozen 100um wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4C.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunohistochemistry-NB600-1254-img0011.jpg)
![ELISA: ROBO1 Antibody [NB600-1254] - ELISA results of purified ROBO1 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/ROBO1-Antibody-ELISA-NB600-1254-img0017.jpg)
![Western Blot: PSRC2 Antibody [NB100-68267] - Detection of Human CCDC131 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC131 antibody NB100-68267 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PSRC2-Antibody-Western-Blot-NB100-68267-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: PSRC2 Antibody [NB100-68267] - Detection of Human CCDC131 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC131 antibody NB100-68267 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>PSRC2 Antibody [NB100-68267] - Detection of human CCDC131/PSRC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCDC131 antibody NB100-68267 (lot 2 ) used for IP at 6 ug per reaction. CCDC131 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-CCDC131 antibody NB100-68265. For blotting immunoprecipitated CCDC131, NB100-68267 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PSRC2-Antibody-NB100-68267-img0007.jpg)
![Western Blot: DAF-3 Antibody [NB100-1924] - Detection of DAF-3 in 1: wildtype C. elegans lysate and 2: DAF-3 deletion C. elegan lysate.](http://images.novusbio.com/fullsize/DAF-3-Antibody-Western-Blot-NB100-1924-img0004.jpg "Western Blot: DAF-3 Antibody [NB100-1924] - Detection of DAF-3 in 1: wildtype C. elegans lysate and 2: DAF-3 deletion C. elegan lysate.")
![Western Blot: KMT2A/MLL Antibody [NB600-256] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/KMT2A-MLL-Antibody-Western-Blot-NB600-256-img0012.jpg "Western Blot: KMT2A/MLL Antibody [NB600-256] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Flow Cytometry: KMT2A/MLL Antibody [NB600-256] - Flow cytometry testing of KMT2A/MLL antibody on murine WT and Mll1-/- CD4 T cells that were stimulated in-vitro with anti-CD3/ -CD28 antibodies. Image submitted by our verified customer (see Review section for additional details).](http://images.novusbio.com/fullsize/KMT2A-MLL-Antibody-Flow-Cytometry-NB600-256-img0010.jpg)
![Chromatin Immunoprecipitation: KMT2A/MLL Antibody [NB600-256] - A. 10 ug of was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256).](http://images.novusbio.com/fullsize/KMT2A-MLL-Antibody-Chromatin-Immunoprecipitation-NB600-256-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: KMT2A/MLL Antibody [NB600-256] - Detection of human MLL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-256 used for IP at 3 ug per reaction. MLL1 was also immunoprecipitated by another rabbit anti-MLL1 antibody. For blotting immunoprecipitated MLL1, NB600-256 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/KMT2A-MLL-Antibody-Immunoprecipitation-NB600-256-img0013.jpg)
![Western Blot: NHP2L1 Antibody [NBP1-32732] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract15 % SDS-PAGE NHP2L1 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/NHP2L1-Antibody-Western-Blot-NBP1-32732-img0008.jpg "Western Blot: NHP2L1 Antibody [NBP1-32732] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract15 % SDS-PAGE NHP2L1 antibody dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: NHP2L1 Antibody [NBP1-32732] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NHP2L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32732-img0004.jpg)
![Immunohistochemistry-Paraffin: NHP2L1 Antibody [NBP1-32732] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/NHP2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-32732-img0005.jpg)
![Western Blot: NHP2L1 Antibody [NBP1-32732] - Sample (30 ug of whole cell lysate)A: HeLaB: Hep G2 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NHP2L1-Antibody-Western-Blot-NBP1-32732-img0006.jpg)
![Western Blot: NHP2L1 Antibody [NBP1-32732] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract.](http://images.novusbio.com/fullsize/NHP2L1-Antibody-Western-Blot-NBP1-32732-img0007.jpg)
![NHP2L1 Antibody [NBP1-32732] - Paraformaldehyde-fixed zebrafish embryos. NHP2L1 antibody [N1C3] dilution: 1:200.](http://images.novusbio.com/fullsize/antibody/nbp1-32732_rabbit-polyclonal-nhp2l1-antibody-immunohistochemistry-whole-mount-8520237549..jpg)
