DAF-3 Antibody

Images

 
Western Blot: DAF-3 Antibody [NB100-1924] - Detection of DAF-3 in 1: wildtype C. elegans lysate and 2: DAF-3 deletion C. elegan lysate.

Product Details

Summary
Reactivity CeSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DAF-3 Antibody Summary

Immunogen
A synthetic peptide made to the C-terminus (within residues 700-796) of the C.elegans DAF-3 protein. [UniProt# O17532]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
This DAF-3 antibody is useful for Chromatin Immunoprecipitation (PMID 16777607), Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot.
Publications
Read Publications using
NB100-1924 in the following applications:

  • 1 publication
  • IP
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for DAF-3 Antibody

  • Abnormal dauer formation protein 3
  • DAF 3
  • DAF3
  • isoform c

Background

DAF-3 is unique in that it is antagonized, rather than activated, by a TGF-beta pathway. In Caenorhabditis elegans (C.elegans), defects in a TGF-beta-related pathway cause a reversible developmental arrest and metabolic shift at the dauer larval stage. DAF-3 transgenes interfere with dauer formation, playing a role in the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAF-3 Antibody (NB100-1924)(2)

We have publications tested in 1 confirmed species: C. elegans.

We have publications tested in 2 applications: ChIP, IP.


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ChIP
(1)
IP
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

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Secondary Antibodies

 

Isotype Controls

Additional DAF-3 Products

DAF-3 NB100-1924

Research Areas for DAF-3 Antibody (NB100-1924)

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Bioinformatics

Entrez
Uniprot