DAF-3 Antibody - BSA Free

Western Blot: DAF-3 Antibody [NB100-1924] - Detection of DAF-3 in 1: wildtype C. elegans lysate and 2: DAF-3 deletion C. elegan lysate.

• Reactivity: Ce
• Applications: WB, ChIP, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DAF-3 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to the C-terminus (within residues 700-796) of the C.elegans DAF-3 protein. [UniProt# O17532]
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 16777607)
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for DAF-3 Antibody - BSA Free

• Abnormal dauer formation protein 3
• DAF 3
• DAF3
• isoform c

Background

DAF-3 is unique in that it is antagonized, rather than activated, by a TGF-beta pathway. In Caenorhabditis elegans (C.elegans), defects in a TGF-beta-related pathway cause a reversible developmental arrest and metabolic shift at the dauer larval stage. DAF-3 transgenes interfere with dauer formation, playing a role in the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAF-3 Antibody (NB100-1924)(3)

Publications using NB100-1924

• Pekar O, Ow MC, Hui KY et al. Linking the environment, DAF-7/TGFb signaling and LAG-2/DSL ligand expression in the germline stem cell niche Development 2017-08-15 [PMID: 28811311] (Chemotaxis, C. elegans)
• Sims JR, Ow MC, Nishiguchi MA et al. Developmental programming modulates olfactory behavior in C. elegans via endogenous RNAi pathways Elife 2016-06-29 [PMID: 27351255] (IP)
• Deplancke, B et al. A gene-centered C. elegans protein-DNA interaction network. Cell. 125(6):1193-205. 2006-06-16 [PMID: 16777607] (Chemotaxis, C. elegans)

Bioinformatics

• Entrez:
  • 180431(C. elegans)
• Uniprot:
  • O17532(C. elegans)