PSRC2 Antibody

Western Blot: PSRC2 Antibody [NB100-68267] - Detection of Human CCDC131 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC131 antibody NB100-68267 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

PSRC2 Antibody [NB100-68267] - Detection of human CCDC131/PSRC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCDC131 antibody NB100-68267 (lot 2 ) used for IP at 6 ug per reaction. CCDC131 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-CCDC131 antibody NB100-68265. For blotting immunoprecipitated CCDC131, NB100-68267 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PSRC2 Antibody Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit PSRC2 Antibody (NB100-68267) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-PSRC2 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1939 and 1989 of human coiled-coil domain containing 131 using the numbering given in entry NP_659419.3 (GeneID 196441).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZFC3H1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Knockout Validated
  • Western Blot 1:2000-1:10000
• Application Notes: Knockout validation (PMID: 31722198). Use in ICC/IF reported in scientific literature (PMID:31950173).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PSRC2 Antibody

• coiled-coil domain containing 131
• Coiled-coil domain-containing protein 131
• DKFZp686A0722
• KIAA0546CCDC131
• MGC23401
• MGC90200
• proline/serine-rich coiled-coil 2
• Proline/serine-rich coiled-coil protein 2
• PSRC2
• zinc finger C3H1 domain-containing protein
• zinc finger, C3H1-type containing

Background

CCDC131 (coiled-coil domain containing protein 131) is an uncharacterized proline/serine rich coiled-coil protein that bears 6 HAT repeats- and 5 TPR repeats. Coiled-coil proteins exhibit tertiary structures made up of a number of highly organized alpha helices wound around each other to mediate oligomerization. HAT (half a TPR) repeats form amphipathic alpha-helices and are similar to TPR repeats which are 34 amino acid repeat motifs which form alpha helical structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSRC2 Antibody (NB100-68267)(7)

Publications using NB100-68267

• Fan J, Wang Y, Wen M, Tong D et Al. Dual modes of ZFC3H1 confer selectivity in nuclear RNA sorting Mol Cell 2024-10-26 [PMID: 39461342]
• Taniue K, Sugawara A, Zeng C et al. The MTR4/hnRNPK complex surveils aberrant polyadenylated RNAs with multiple exons Nature Communications 2024-10-17 [PMID: 39419981]
• Wang Y, Fan J, Wang J et al. ZFC3H1 prevents RNA trafficking into nuclear speckles through condensation Nucleic acids research 2021-09-16 [PMID: 34530450] (WB, ICC/IF)
• Silla T, Schmid M, Dou Y et al. The human ZC3H3 and RBM26/27 proteins are critical for PAXT-mediated nuclear RNA decay Nucleic Acids Res. 2020-01-17 [PMID: 31950173] (ICC/IF, Human)
• Lloret-Llinares M, Jensen TH Global Identification of Human Exosome Substrates Using RNA Interference and RNA Sequencing Methods Mol. Biol. 2019-11-27 [PMID: 31768975] (WB, Human)
• Garland W, Comet I, Wu M et al. A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2 Cell Rep 2019-11-12 [PMID: 31722198] (KO)
• Toomas Silla, Evdoxia Karadoulama, Dawid Mąkosa, Michal Lubas, Torben Heick Jensen The RNA Exosome Adaptor ZFC3H1 Functionally Competes with Nuclear Export Activity to Retain Target Transcripts Cell Reports 2018-05-15 [PMID: 29768216]

Bioinformatics

• Gene Symbol: ZFC3H1
• Entrez:
  • 196441(Human)
• Uniprot:
  • NP_659419.3(Human)