Phospho-Tyrosine Antibody (179003) [Biotin]

221-AA), Recombinant Human PDGF-AB (Catalog # 222-AB), or Recombinant Human PDGF-BB (Catalog # 222-BB) for 5 minutes at 37 °C in serum-free medium. Activation of PDGF R beta was assessed by ELISA using Human PDGF R beta Antibody (Catalog # MAB385) as the capture reagent and Phospho-Tyrosine Biotinylated Monoclonal Antibody (Catalog # BAM1676) as the detection reagent." > 221-AA), Recombinant Human PDGF-AB (Catalog # 222-AB), or Recombinant Human PDGF-BB (Catalog # 222-BB) for 5 minutes at 37 °C in serum-free medium. Activation of PDGF R beta was assessed by ELISA using Human PDGF R beta Antibody (Catalog # MAB385) as the capture reagent and Phospho-Tyrosine Biotinylated Monoclonal Antibody (Catalog # BAM1676) as the detection reagent." title="Human Foreskin Fibroblasts were untreated or treated with 100 ng/mL Recombinant Human PDGF-AA (Catalog # 221-AA), Recombinant Human PDGF-AB (Catalog # 222-AB), or Recombinant Human PDGF-BB (Catalog # 222-BB) for 5 minutes at 37 °C in serum-free medium. Activation of PDGF R beta was assessed by ELISA using Human PDGF R beta Antibody (Catalog # MAB385) as the capture reagent and Phospho-Tyrosine Biotinylated Monoclonal Antibody (Catalog # BAM1676) as the detection reagent." />

Human Foreskin Fibroblasts were untreated or treated with 100 ng/mL Recombinant Human PDGF-AA (Catalog # <a class=

HAF007). Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10." > HAF007). Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10." title="Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Phospho-Tyrosine Biotinylated Monoclonal Antibody (Catalog # BAM1676), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10." />

Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Phospho-Tyrosine Biotinylated Monoclonal Antibody (Catalog # BAM1676), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

• Reactivity: All-Multi
• Applications: WB, ELISA
• Clone: 179003
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

Phospho-Tyrosine Antibody (179003) [Biotin] Summary

• Immunogen: KLH-coupled Phospho-Tyrosine synthetic peptide
• Specificity: Detects proteins containing phosphorylated tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. The antibody does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA 0.75 ug/mL
  • Western Blot 1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Phospho-Tyrosine Antibody (179003) [Biotin]

• 2 amino 3(4 hydroxyphenyl) propanoic acid
• 4 hydroxyphenylalanine
• PhosphoTyrosine
• Phospho-Tyrosine
• pTyrosine
• pY20
• Tyrosine

Background

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Phospho-Tyrosine Antibody (BAM1676)(4)

Publications using BAM1676

• Creixell, M;Taylor, SD;Gerritsen, J;Bae, SY;Jiang, M;Augustin, T;Loui, M;Boixo, C;Creixell, P;White, FM;Meyer, AS; Dissecting signaling regulators driving AXL-mediated bypass resistance and associated phenotypes by phosphosite perturbations bioRxiv : the preprint server for biology 2023-10-23 [PMID: 37961516] (Human)
• C Yilmaz, T Rogdakis, A Latorrata, E Thanou, E Karadima, E Papadimitr, E Siapi, KW Li, T Katsila, T Calogeropo, I Charalampo, VI Alexaki ENT-A010, a Novel Steroid Derivative, Displays Neuroprotective Functions and Modulates Microglial Responses Biomolecules, 2022-03-09;12(3):. 2022-03-09 [PMID: 35327616] (Western Blot, Rat)
• T Rogdakis, D Charou, A Latorrata, E Papadimitr, A Tsengenes, C Athanasiou, M Papadopoul, C Chalikiopo, T Katsila, I Ramos, KC Prousis, RC Wade, K Sidiropoul, T Calogeropo, A Gravanis, I Charalampo Development and Biological Characterization of a Novel Selective TrkA Agonist with Neuroprotective Properties against Amyloid Toxicity Biomedicines, 2022-03-06;10(3):. 2022-03-06 [PMID: 35327415] (Western Blot, Human, Rat)
• Reinhard M, Frystyk J, Jespersen B, Bjerre M, Christiansen J, Flyvbjerg A, Ivarsen P Effect of hyperinsulinemia during hemodialysis on the insulin-like growth factor system and inflammatory biomarkers: a randomized open-label crossover study. BMC Nephrol, 2013-04-04;14(0):80. 2013-04-04 [PMID: 23557110] (ELISA Development (Detection), Human)