AU1 Epitope Tag Antibody - BSA Free

Western Blot: AU1 Epitope Tag Antibody [NB600-452] - Western Blot: AU1 Epitope Tag Antibody [HRP] [NB600-457] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.2 ug/ml (1:5,000). Image using the HRP form of this antibody.

• Reactivity: ET
• Applications: WB, ELISA, Func, ICC/IF, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

AU1 Epitope Tag Antibody - BSA Free Summary

• Immunogen: Goats were immunized with AU1 (DTYRYI) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:500
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:400
  • Immunoprecipitation 1 - 4 ug/mg lysate
  • Proximity Ligation Assay
  • Western Blot 1:1000 - 1:3000
• Application Notes: Use in PLA reported in scientific literature (PMID: 32690953).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AU1 Epitope Tag Antibody - BSA Free

• AU1 epitope tag
• DTYRYI epitope tag
• DTYRYI tag

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AU1 Epitope Tag Antibody (NB600-452)(8)

Publications using NB600-452

• Huang YC, Su LH, Lee GA et al. Regulation of cyst wall protein promoters by Myb2 in Giardia lamblia J Biol Chem 2008-11-01 [PMID: 18768462]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Toulouse A, Au-Yeung F, Gaspar C et al. Ribosomal frameshifting on MJD-1 transcripts with long CAG tracts Hum Mol Genet 2005-09-01 [PMID: 16087686]
  
  Details:
  This citation used the FITC version of this antibody.
• Huang YC, Su LH, Lee GA et al. Regulation of cyst wall protein promoters by Myb2 in Giardia lamblia J Biol Chem
  
  Details:
  This publication used the Agarose Immobilized conjugated form of this antibody (Cat# NB600-400).
• Maxime C. van Zwam, Anubhav Dhar, Willem Bosman, Wendy van Straaten, Suzanne Weijers, Emiel Seta, Ben Joosten, Jeffrey van Haren, Saravanan Palani, Koen van den Dries, Carole A. Parent IntAct: A nondisruptive internal tagging strategy to study the organization and function of actin isoforms PLOS Biology 2024-03-11 [PMID: 38466773]
• Volcic M, Sparrer KMJ, Koepke L et al. Vpu modulates DNA repair to suppress innate sensing and hyper-integration of HIV-1 Nat Microbiol 2020-07-20 [PMID: 32690953] (PLA)
• Ermler ME, Yerukhim E, Schriewer J et al. RNA helicase signaling is critical for type I interferon production and protection against Rift Valley fever virus during mucosal challenge. J Virol 2013-02-13 [PMID: 23408632] (WB, Human)
• Sarkisian MR, Siebzehnrubl D. Abnormal levels of Gadd45alpha in developing neocortex impair neurite outgrowth. PLoS One 2012-01-01 [PMID: 22970179]
• Chen W, Luo Y, Liu L et al. Cryptotanshinone inhibits cancer cell proliferation by suppressing Mammalian target of rapamycin-mediated cyclin D1 expression and Rb phosphorylation. Cancer Prev Res (Phila) 2010-08-01 [PMID: 20628002]

FAQs for AU1 Epitope Tag Antibody (NB600-452). (Showing 1 - 1 of 1 FAQs).

1. I am interested in testing one of your antibodies for a Western blot and was wondering if it would be possible to get a small sample to test before purchasing? The antibody in question is AU1 Epitope Tag Antibody (NB600-452). Please let me know if this could happen.
   • It is company policy that we do not send out free samples as we have a 100% guarantee on our products for all applications and species listed on the datasheet. NB600-452 is guaranteed for use in Western blot, ELISA, immunoprecipitation and ICC.