Phospho-Tyrosine Antibody (179003) [Alkaline Phosphatase]

Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1:1000 dilution of Phospho-Tyrosine AP-conjugated Monoclonal Antibody (Catalog # APM1676), followed by [Secondary Antibody Proper Name & Link]. Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.

• Reactivity: All-Multi
• Applications: WB, ELISA
• Clone: 179003
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alkaline Phosphatase

Phospho-Tyrosine Antibody (179003) [Alkaline Phosphatase] Summary

• Immunogen: KLH-coupled Phospho-Tyrosine synthetic peptide
• Specificity: Detects proteins containing phosphorylated Tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that it binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. This antibody does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) with stabilizers and preservatives.
• Preservative: No Preservative
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Phospho-Tyrosine Antibody (179003) [Alkaline Phosphatase]

• 2 amino 3(4 hydroxyphenyl) propanoic acid
• 4 hydroxyphenylalanine
• PhosphoTyrosine
• pTyrosine
• pY20
• Tyrosine

Background

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.