Western blot shows lysates of human colon tissue, SK‑BR‑3 human breast cancer cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cystatin B ...read more
Papain (0.1 µg/mL) activity is measured in the presence of Recombinant Human Cystatin B (0.53 µg/mL, Catalog # 1408-PI) that has been preincubated with increasing concentrations of Human Cystatin B Monoclonal Antibody ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Cystatin B knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse ...read more
Cystatin B/Stefin B Antibody (225228) [Unconjugated] Summary
E. coli-derived recombinant human Cystatin B aa Met2-Phe98 Accession # P04080
Detects human Cystatin B in Western blots and direct ELISAs. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Cystatins A and S, recombinant mouse Cystatin B, rhFetuin A, and rhHPRG (Histidine-Proline-Rich Glycoprotein) is observed and approximately 5% to 10% cross-reactivity with rhCystatins C, D, E/M, SA, SN, and rhFetuin B is observed.
Protein A or G purified from hybridoma culture supernatant
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Cystatin B/Stefin B Antibody (225228) [Unconjugated]
CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B
cystatin B (stefin B)
Liver thiol proteinase inhibitor
Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Mutations in the Cystatin B gene is the cause of progressive myoclonus epilepsy (EPM1) (3). Because of its expression patterns, Cystatin B can be used as a marker for certain cancers, such as glioblastoma tumors (4). It readily forms amyloid fibrils in vitro (5). The human Cystatin B consists of 98 amino acid residues (3).
Abrahamson, M. (1994) Methods Enzymol. 244:685.
Pol, E. and I. Bjork (1999) Biochemistry 38:10519.
Pennacchio, L.A. et al. (1996) Science 271:1731.
Zhang, R. et al. (2003) Glia 42:194.
Zerovnik, E. et al. (2002) Biochim. Biophys. Acta 1594:1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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