Phospho-Tyrosine Antibody (179003) [Alexa Fluor® 488]

• Reactivity: All-Multi
• Applications: WB, IP, ICC/IF
• Clone: 179003
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Phospho-Tyrosine Antibody (179003) [Alexa Fluor® 488] Summary

• Immunogen: KLH-coupled phospho-tyrosine synthetic peptide
• Specificity: Detects proteins containing phosphorylated tyrosine residues. ELISA and Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds phospho-tyrosine in a broad manner largely independent of the surrounding amino acid sequ
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Phospho-Tyrosine Antibody (179003) [Alexa Fluor® 488]

• 2 amino 3(4 hydroxyphenyl) propanoic acid
• 4 hydroxyphenylalanine
• PhosphoTyrosine
• pTyrosine
• pY20
• Tyrosine

Background

Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal cell cycle progression and oncogenic transformation. The role  of tyrosine phosphorylation is a matter of active and ongoing research. Specific antibodies that  recognize phosphorylated tyrosine residues are valuable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they are involved.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.