Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22164SS | |||
| Azide and BSA Free | NBP2-80954 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0361A488-100 | |||
| Alexa Fluor 546 | DDX0361A546-100 | |||
| Alexa Fluor 647 | DDX0361A647-100 | |||
| Biotin | DDX0361B-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47339SS | |||
| Azide and BSA Free | NBP2-80748 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77836SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-351SS | |||
| Azide and BSA Free | NBP2-80929 | |||
![Western Blot: SLC6A3/DAT1 Antibody (mAb16) [NBP2-22164] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review.](http://images.novusbio.com/fullsize/SLC6A3-DAT1-Antibody-mAb16-Western-Blot-NBP2-22164-img0005.jpg "Western Blot: SLC6A3/DAT1 Antibody (mAb16) [NBP2-22164] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review.")
![Immunocytochemistry/Immunofluorescence: DAT1 Antibody (mAb16) [NBP2-22164] - The DAT1 antibody was tested in PC12 cells at a 1:250 dilution against DyLight 488 (Green). Actin and nuclei were counterstained with Phalloidin-AlexaFluor 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/DAT1-Antibody-mAb16-Immunocytochemistry-Immunofluorescence-NBP2-22164-img0004.jpg)
![Immunohistochemistry-Paraffin: DAT1 Antibody (mAb16) [NBP2-22164] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/DAT1-Antibody-mAb16-Immunohistochemistry-Paraffin-NBP2-22164-img0002.jpg)
![Western Blot: DAT1 Antibody (mAb16) [NBP2-22164] - Analysis of DAT1 expression in rat striatal membrane tissue lysate.](http://images.novusbio.com/fullsize/DAT1-Antibody-mAb16-Western-Blot-NBP2-22164-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Cryosections of human skin, 1:200 dilution, staining for 1 hour at 37 degrees. Dapi nuclear stain used to define architecture. Langerin staining is visible in the epidermis. Image submitted from verified customer review.](http://images.novusbio.com/fullsize/Langerin-CD207-Antibody-310F7-02-Immunocytochemistry-Immunofluorescence-DDX0361P-100-img0018.jpg "Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Cryosections of human skin, 1:200 dilution, staining for 1 hour at 37 degrees. Dapi nuclear stain used to define architecture. Langerin staining is visible in the epidermis. Image submitted from verified customer review.")
![Immunohistochemistry-Paraffin: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Staining of human skin paraffin section with 310F7.02](http://images.novusbio.com/fullsize/Langerin-CD207-Antibody-310F7-02-Immunohistochemistry-Paraffin-DDX0361P-100-img0014.jpg)
![Flow Cytometry: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Saining with 310F7.02 o f cells derived from overnight culture o f porcine skin biopsies permeabilized (B) or not(A).](http://images.novusbio.com/fullsize/Langerin-CD207-Antibody-310F7-02-Flow-Cytometry-DDX0361P-100-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Staining of CHO cells transfected (right) or not (left) with mouse Langerin.](http://images.novusbio.com/fullsize/Langerin-CD207-Antibody-310F7-02-Immunofluorescence-DDX0361P-100-img0017.jpg)
![Immunohistochemistry-Paraffin: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Porcine skin cryosections stained with Langerin antibody](http://images.novusbio.com/fullsize/Langerin-CD207-Antibody-310F7-02-Immunohistochemistry-Paraffin-DDX0361P-100-img0004.jpg)
![Flow (Cell Surface): Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Analysis using the Allophycocyanin conjugate of DDX0361P-100. Staining of Langerin in stably transfected CHO using Langerin antibody at 1ug/ml. Blue histogram represents isotype control (1ug/ml), red histogram is anti-Langerin. Total viable cells were use](http://images.novusbio.com/fullsize/Langerin-CD207-Antibody-310F7-02-Flow-Cell-Surface-DDX0361P-100-img0008.jpg)
![Flow (Intracellular): Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Analysis using the PE conjugate of DDX0361P-100. Staining of Langerin in stably transfected CHO cells (A) or DC-SIGN in stably transfected HeLa cells (negative control) (B) using antibody at 1ug/ml. Blue histogram in both panels represents isotype control.](http://images.novusbio.com/fullsize/Langerin-CD207-Antibody-310F7-02-Flow-Intracellular-DDX0361P-100-img0009.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0031.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Cellular aggregates composed of equine endometrial fibroblast. Co-staining using the vimentin antibody and a cytokeratin antibody (mouse). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunocytochemistry-NBP1-31327-img0037.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Human primary cardiac fibroblasts stained with Vimentin (green) and smooth muscle actin (red) antibodies. Image from verified customer review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31327-img0024.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat ovary. Vimentin antibody at 1:500.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0036.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - 293T cell lysate: A. 20 ug. B. 10 ug. C. 5 ug. D. 1 ug.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0014.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, membrane was blotted with Vimentin antibody at 1:50000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0023.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded human lung adenocarcinoma. Vimentin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0022.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat testis. Vimentin antibody at 1:500.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0034.jpg)
![Immunoprecipitation: Vimentin Antibody [NBP1-31327] - Vimentin protein from HeLa whole cell extracts using 5 ug of Vimentin antibody. Western blot analysis was performed using Vimentin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunoprecipitation-NBP1-31327-img0019.jpg)
![Sandwich ELISA: Vimentin Antibody [NBP1-31327] - Detection of recombinant full-length Vimentin protein using as capture antibody at concentration of 5 ug/mL as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Sandwich-ELISA-NBP1-31327-img0039.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-9102024204128.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-91020242010448.jpg)
![ELISA: Vimentin Antibody [NBP1-31327] - Sandwich ELISA detection of recombinant full-length Vimentin protein using the capture antibody at concentration of 5 ug/mL and NBP1-31327 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-91020242063381.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202419593530.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420131228.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.Sample:Paraffin-Embedded adult mouse retina. Green: Vimentin protein stained by Vimentin antibody (NBP1-31327) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420131227.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-9102024201149.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoskeleton and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-91020242011456.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-91020242021974.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat ovary.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420134066.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420134020.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein by immunohistochemical analysis.Sample: Paraffin-embedded dog pancreas.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420115052.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoskeleton by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420134098.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP1-31327] - Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-1610202419414514.jpg)
![Western Blot: GAPDH Antibody (1A10) [NBP1-47339] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Western-Blot-NBP1-47339-img0006.jpg "Western Blot: GAPDH Antibody (1A10) [NBP1-47339] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) [NBP1-47339] - Analysis of methanol-fixed HepG2 (left) and HeLa (right) cells using anti-GAPDH mAb (green), showing cytoplasmic localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunocytochemistry-Immunofluorescence-NBP1-47339-img0004.jpg)
![Flow Cytometry: GAPDH Antibody (1A10) [NBP1-47339] - An intracellular stain was performed on U-87 cells with GAPDH [1A10] Antibody NBP1-47339AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Flow-Cytometry-NBP1-47339-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) [NBP1-47339] - GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 2 ug/mL of mouse anti-GAPDH monoclonal (NB300-328), 2 ug/mL mL of mouse anti- GAPDH monoclonal (NBP1-47339). Cells were stained using the appropriate secondary antibody donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunocytochemistry-Immunofluorescence-NBP1-47339-img0008.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (1A10) [NBP1-47339] - Analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using anti-GAPDH mAb with DAB staining.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunohistochemistry-Paraffin-NBP1-47339-img0005.jpg)
![Simple Western: GAPDH Antibody (1A10) [NBP1-47339] - Lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted 36 kDa molecular weight.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Simple-Western-NBP1-47339-img0007.jpg)
![Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-Western-Blot-NB600-326-img0001.jpg "Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).")
![Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hUbiquitn-GST (left) & hISG15-GST (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-Simple-Western-NB600-326-img0004.jpg)
![Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hUbiquitn-GST (left) & hISG15-GST (right) lysate(s).](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-Simple-Western-NB600-326-img0005.jpg)
![Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-Simple-Western-NB600-326-img0006.jpg)
![Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s).](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-Simple-Western-NB600-326-img0007.jpg)
![N/A P-Selectin/CD62P [HRP]](http://images.novusbio.com/fullsize2/DATA_PSelectin_MPS00_ELISA_1017.jpg "N/A P-Selectin/CD62P [HRP]")
![N/A P-Selectin/CD62P [HRP]](http://images.novusbio.com/fullsize2/P-Selectin_MPS00_ELISA_309.jpg)
![N/A G-CSF [Biotin]](http://images.novusbio.com/fullsize2/DATA_GCSF_DY214_ELISA_1772.jpg "N/A G-CSF [Biotin]")
![N/A IL-2 [HRP]](http://images.novusbio.com/fullsize2/DATA_IL2_R2000_ELISA_1082.jpg "N/A IL-2 [HRP]")
![N/A IL-2 [HRP]](http://images.novusbio.com/fullsize2/IL-2_PR2000_ELISA_348.jpg)
![N/A Flt-3 Ligand/FLT3L [HRP]](http://images.novusbio.com/fullsize2/DATA_Flt3_Ligand_DFK00_ELISA_675.jpg "N/A Flt-3 Ligand/FLT3L [HRP]")
![N/A Flt-3 Ligand/FLT3L [HRP]](http://images.novusbio.com/fullsize2/DATA_Flt3_Ligand_DFK00_ELISA_676.jpg)
![N/A Flt-3 Ligand/FLT3L [HRP]](http://images.novusbio.com/fullsize2/Flt-3_Ligand_DFK00_ELISA_93.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF (+lanes) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein. Personal Communication. YIgG Zhang, NIH, CCR, Bethesda, MD.](http://images.novusbio.com/fullsize/Smad3-[p-Ser423--p-Ser425]-Antibody-Western-Blot-NBP1-77836-img0005.jpg "Western Blot: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF (+lanes) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein. Personal Communication. YIgG Zhang, NIH, CCR, Bethesda, MD.")
![Immunocytochemistry/Immunofluorescence: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - BH4 precursor, sepiapterin, normalizes NO level and ameliorates malignant phenotype of precancerous and cancerous cells. Representative images of the progression series cultivated in 3D ECM with or without sepiapterin (20 uM) and stained for phospho-SMAD3 or ERBB2. Nuclei were counterstained with DAPI (blue). Scale bars: 20 um. (Bottom) Quantification of phospho-SMAD3 (left) and ERBB2 levels (right) of the progression series. Error bars: mean +/- STDEV. *p 2 groups; t-test for 2 groups Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36717814), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77836_rabbit-polyclonal-smad3-p-ser423-p-ser425-antibody-western-blot-13220261525248.jpg)
![N/A MMP-2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_MMP2_DY902_ELISA_2256.jpg "N/A MMP-2 [Biotin]")
![Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] - Analysis for RNase L in WT and eIF2a-S51A MEF cell lines transduced with RNase L-encoding lentivirus. n.s. indicates nonspecific band for the purpose of showing equal loading. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31896577/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RNase-L-Antibody-2E9-BSA-Free-Western-Blot-NB100-351-img0010.jpg "Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] - Analysis for RNase L in WT and eIF2a-S51A MEF cell lines transduced with RNase L-encoding lentivirus. n.s. indicates nonspecific band for the purpose of showing equal loading. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31896577/) licensed under a CC-BY license.")
![Immunohistochemistry: RNase L Antibody (2E9) [NB100-351] - RNase L antibody was tested in human colon using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/RNase-L-Antibody-2E9-Immunohistochemistry-NB100-351-img0006.jpg)
![Western Blot: RNase L Antibody (2E9) [NB100-351] - RNaseL in insect cell extracts transfected with recombinant human RNase L (0.1 ug) and Hey1B (100 ug)](http://images.novusbio.com/fullsize/RNase-L-Antibody-2E9-Western-Blot-NB100-351-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] - Analysis of PKR and RNase L in parental (WT), RNase L-KO (RL-KO), PKR-KO, and RNase L/PKR double knockout (RL/PKR-KO) A549 cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31896577/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RNase-L-Antibody-2E9-BSA-Free-Knockout-Validated-NB100-351-img0009.jpg)
![Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] - dsRNA-induced SGs require G3BP1 & G3BP2, whereas RLBs do not.A, immunoblot for G3BP1, RNase L, GAPDH in parental (WT), RL-KO, G3BP-KO, & RL/G3BP-KO U-2 OS cells. B, immunoblot for G3BP1 & G3BP2 in RL-KO & RL/G3BP-KO U-2 OS cells. C, IF for G3BP1 & PABPC1 4 h post-poly(I·C) transfection or treatment with 500 μm sodium arsenite (SA) in the indicated cell lines. D, similar to C but enlarged to show RLB formation in U-2 OS-G3BP1/2-KO cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31896577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-351_mouse-monoclonal-rnase-l-antibody-2e9-310202415171326.jpg)