Dopa Decarboxylase/DDC Antibody

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Summary
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    • Catalog Number
      NB100-1785
    • Availability
      Product Discontinued

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Dopa Decarboxylase/DDC Antibody Summary

Immunogen
Peptide with sequence C-WEHIKELAADVL corresponding to C-Terminus according to NP_000781.1, NP_001229815.1, NP_001229816.1, NP_001229817.1, NP_001229818.1.
Specificity
This antibody is expected to recognise isoforms 1, 2, 3, 4 and 5 (NP_000781.1; NP_001229815.1; NP_001229816.1; NP_001229817.1; NP_001229818.1 respectively). Reported variants represent identical protein (NP_000781.1; NP_001076440.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DDC
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in human kidney lysates and 48 kDa band on human brain lysates (calculated MW of 53.9 kDa band according to NP_000781.1).
Control
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-1785.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dopa Decarboxylase/DDC Antibody

  • AADC
  • AADCaromatic-L-amino-acid decarboxylase
  • DDC
  • dopa decarboxylase (aromatic L-amino acid decarboxylase)
  • Dopa Decarboxylase
  • EC 4.1.1
  • EC 4.1.1.28

Background

DOPA decarboxylase is an enzyme implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters: dopamine and serotonin which both play key roles in many clinical disorders, including Parkinson's disease. Following the hydroxylation of tyrosine to form L dihydroxyphenylalanine (LDOPA), catalyzed by tyrosine hydroxylase, DDC decarboxylates LDOPA to form dopamine. This neurotransmitter is found in different areas of the brain and is particularly abundant in basal ganglia. Dopamine is also produced by DDC in the sympathetic nervous system and is the precursor of the catecholaminergic hormones, noradrenaline and adrenaline in the adrenal medulla. In the nervous system, tryptophan hydroxylase produces 5 OH tryptophan, which is decarboxylated by DDC, giving rise to serotonin. DDC is a homodimeric, pyridoxal phosphate dependent enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dopa Decarboxylase/DDC Antibody (NB100-1785)(1)


Showing Publication 1 - 1 of 1.
Publication using NB100-1785 Applications Species
Voeller KK. Attention-deficit hyperactivity disorder (ADHD). J Child Neurol 2004-10-01 [PMID: 15559895]

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Bioinformatics

Gene Symbol DDC
Entrez
Uniprot