IgG (H+L) Antibodies
Immunoglobulin G (IgG), expressed on the surface of mature B cells, is often depicted as a Y-shape containing 2 heavy chains (H) and 2 light chains (L) linked by disulfide bonds. Browse our extensive catalog for polyclonal antibodies and IgG (H+L) conjugated secondary antibodies including FITC, HRP, and Biotin. Validated research applications include ChIP, ELISA, ICC, Immunofluorescence, IHC, IHC-Paraffin, IHC-Frozen, Flow Cytometry, IP, and Western Blot. Our IgG (H+L) secondary antibodies can be used in a variety of model species including Human, Mouse, Rat, Rabbit, Bovine, Canine, Chicken, Donkey, Equine, Feline, Goat, Llama, Porcine, and Sheep. Choose the anti-IgG (H+L) antibody best suited for your research needs by viewing the technical details, images, references, reviews and related products. Each IgG (H+L) antibody is fully covered by our Guarantee+.
Alternate Names for IgG (H+L) Antibodies
300 Results for "igg-(h-l)" in Secondary Antibodies
![Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Swine IgG. Other band(s): None.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Western-Blot-NBP1-72700-img0009.jpg "Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Swine IgG. Other band(s): None.")
![Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: Swine Antibody at 1:1,000 o/n at 4C. Secondary antibody: DyLight 649 goat secondary antibody at 1:20,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Western-Blot-NBP1-72700-img0003.jpg)
![Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: Biotin conjugated swine antibody at 1:1,000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 and 28 kDa for Swine IgG. Other band(s): none. Image from the Biotin version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Western-Blot-NBP1-72700-img0006.jpg)
![Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein swine secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Western-Blot-NBP1-72700-img0007.jpg)
![Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Texas Red swine secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none. Image from the Texas Red version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Western-Blot-NBP1-72700-img0008.jpg)
![Dot Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Dot Blot of Goat anti-Porcine IgG (H+L) Secondary Antibody [Alkaline Phosphatase]. Antigen: Swine IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Incubated for 1hr at RT; blocked for 60 min at RT. Image from the Alkaline Phosphatase version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Dot-Blot-NBP1-72700-img0004.jpg)
![Dot Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Load: 200 ug followed by a 3-fold serial dilution. Primary antibody: none. Secondary antibody: Fluorescien swine secondary antibody at 1:1,000 for 60 min at RT. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Dot-Blot-NBP1-72700-img0005.jpg)
![Western Blot: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - Lane 1: Molecular Weigh Ladder. Lane 2: Guinea Pig IgG. Lane 3: Bovine Serum. Lane 4: Chicken Serum. Lane 5: Goat Serum. Lane 6: Hamster Serum. Lane 7: Horse Serum. Lane 8: Human Serum. Lane 9: Mouse Serum. Lane 10: Rabbit Serum. Lane 11: Rat Serum. Lane 12: Sheep Serum. Loading: 50ng IgG, Serum at 1:8 dilution. Incubated in blocking buffer for 30 min at RT. Secondary Antibody: NB120-7139. Image from the HRP version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NB120-7134-img0004.jpg "Western Blot: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - Lane 1: Molecular Weigh Ladder. Lane 2: Guinea Pig IgG. Lane 3: Bovine Serum. Lane 4: Chicken Serum. Lane 5: Goat Serum. Lane 6: Hamster Serum. Lane 7: Horse Serum. Lane 8: Human Serum. Lane 9: Mouse Serum. Lane 10: Rabbit Serum. Lane 11: Rat Serum. Lane 12: Sheep Serum. Loading: 50ng IgG, Serum at 1:8 dilution. Incubated in blocking buffer for 30 min at RT. Secondary Antibody: NB120-7139. Image from the HRP version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - Immunocytochemistry/Immunofluorescence: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72854]](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NB120-7134-img0005.jpg)
![Dot Blot: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Goat anti-Guinea Pig IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Blocked with blocking buffer for 60 min at RT. Visualized using Alkaline Phosphatase Substrate for 30 seconds at RT. Image from the Alkaline Phosphatase version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NB120-7134-img0001.jpg)
![Dot Blot: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - Dots are Guinea Pig IgG: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat Anti-Guinea Pig IgG Antibody FITC at 1ug/mL in blocking buffer for 1hr at RT. Imaged with BioRad ChemiDoc, Fluorescein filter. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NB120-7134-img0002.jpg)
![ELISA: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NB120-7134-img0009.jpg)
![ELISA: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - ELISA Results of Goat anti-Guinea Porcine IgG (H+L) Secondary antibody (Pre-adsorbed) tested against purified Guinea Porcine MX10. Each well was coated in duplicate with 1.0 ug of Guinea Porcine IgG. The working dilution is 1:51,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Donkey Anti-Goat IgG HRP conjugated, and TMB substrate.](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NB120-7134-img0010.jpg)