Sheep anti-Human IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-absorbed)

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Electron Microscopy: Sheep anti-Human IgG (H+L) Secondary Antibody [Alkaline Phosphatase] [NBP1-72572] - Dot Blot of Sheep anti-Human IgG (H+L) Secondary Antibody [Alkaline Phosphatase]. Antigen: Human IgG. Load: Lane 1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, DB, ELISA, EM, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Sheep
Conjugate
Alkaline Phosphatase
Format
Pre-absorbed
Concentration
1 mg/ml

Order Details

Sheep anti-Human IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-absorbed) Summary

Immunogen
Human IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum, Human IgG and Human Serum.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2500
  • ELISA 1:2000 - 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Frozen 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Application Notes
This product is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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