Rabbit anti-Goat IgG (H+L) Secondary Antibody [HRP]


Western Blot: Rabbit anti-Goat IgG (H+L) Secondary Antibody [HRP] [NBP1-73515] - Lane 1: Goat IgG. Lane 2: Human IgG. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary ...read more

Product Details

Reactivity GtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
LYOPH mg/ml

Order Details

Rabbit anti-Goat IgG (H L) Secondary Antibody [HRP] Summary

Goat IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
0.01% Gentamicin Sulfate
LYOPH mg/ml
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.


  • Western Blot 1:1000 - 1:5000
  • ELISA 1:20000 - 1:120000
  • Immunohistochemistry 1:500 - 1:2500
  • Immunohistochemistry-Paraffin 1:500 - 1:2500
Application Notes
Suitable for immunoblotting ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Readout System


Label Molecular Weight: 40,000, Restore with 1.0 mL of deionized water (or equivalent).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol

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