Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody [FITC]

Images

 
Western Blot: Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody [FITC] [NB120-5743] - Western Blot of Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody [FITC].Lane 1: Armenian Hamster IgG. Lane 2: none. ...read more

Product Details

Summary
Reactivity ArHaSpecies Glossary
Applications WB, FLISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
FITC

Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody [FITC] Summary

Immunogen
This Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody [FITC] was developed against Armenian Hamster IgG whole molecule.
Specificity
Assay of Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody [FITC] by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat serum, Armenian Hamster IgG and Armenian Hamster serum. Minimal reactivity will occur against Golden Syrian Hamster IgG.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
Application Notes
This product is designed for FLISA and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

Storage
Store at -20C in the dark. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB120-5743) (0)

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Other Available Formats

Alkaline Phosphatase NB120-5746
Allophycocyanin/Cy7 NB120-5738APCCY7
Biotin NB120-5744
DyLight 488 NB120-5738G
DyLight 550 NB120-5738R
DyLight 594 NB120-5738DL594
DyLight 650 NB120-5738C
DyLight 680 NB120-5738FR
DyLight 755 NB120-5738IR
FITC NB120-5743
HRP NB100-2066
Janelia Fluor 549 NB120-5738JF549
Janelia Fluor 646 NB120-5738JF646
PE/Atto594 NB120-5738PEATT594
PE/Cy5.5 NB120-5738PECY55
PE/Cy7 NB120-5738PECY7
Rhodamine NB120-5741
Texas Red NBP1-73458

Additional IgG (H+L) Products

Array NB120-5743

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