Donkey anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 800] (Pre-absorbed)

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Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 800] [NBP1-72970] - DyLight (R) dyes can be used for two-color Western Blot detection with low background and high signal.
Fluorophore-linked immunosorbent assay: Donkey anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 800] [NBP1-72970]

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, FLISA, ICC/IF
Clonality
Polyclonal
Host
Donkey
Conjugate
DyLight 800
Format
Pre-absorbed
Concentration
LYOPH

Donkey anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 800] (Pre-absorbed) Summary

Immunogen
Rabbit IgG, whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-72970 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 794, Excitation Wavelength: 770, Label Molecular Weight: 1,050, Restore with 0.1 mL of deionized water (or equivalent).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Review for IgG (H+L) Antibody (NBP1-72970) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 application: ICC/IF.

Reviews using NBP1-72970:
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Immunocytochemistry/Immunofluorescence IgG (H+L) NBP1-72970
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reviewed by:
Christopher England
ICC/IF 12/05/2016
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Lot3320

Comments

CommentsPart of the lot number had been torn off, so it may be missing something. We used the antibody at a concentration of 1:5000.

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Christopher England
12/05/2016
Application: ICC/IF
Species: