Rabbit anti-Rat IgG (H+L) Secondary Antibody [HRP]

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Western Blot: Rabbit anti-Rat IgG (H+L) Secondary Antibody [HRP] [NB120-7104] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Rabbit anti-Rat IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
Full length native Rat IgG (purified).
Localization
Secreted
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Peroxidase, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • ELISA 1:2000 - 1:10000
  • Immunohistochemistry 1:500 - 1:2000
Readout System

Reactivity Notes

Minimum Cross Reactivity to Human Serum Proteins

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

FAQs for IgG Antibody (NB120-7104). (Showing 1 - 1 of 1 FAQ).

  1. I have some questions regarding product number NB120-7104 (rat IgG antibody, HRP). Does this antibody come lyophilized or as a ready to use solution? Is this antibody compatible with primary antibody number NB120-10454 (caspase 11 antibody)?
    • In regards to your inquiries, this antibody does come in liquid form, not in powdered lyophilized form. The antibody will be compatible with NB120-10454 even though that antibody has a more specific isotype the general Rat IgG secondary should still detect the primary.

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