Donkey anti-Sheep IgG (H+L) Secondary Antibody [HRP]

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ELISA: Donkey anti-Sheep IgG (H+L) Secondary Antibody [HRP] [NBP1-73267] - ELISA results of purified Donkey anti-Sheep IgG Antibody Peroxidase Conjugated tested against purified Sheep IgG. Each well was coated in ...read more

Product Details

Summary
Reactivity ShSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP
Concentration
LYOPH

Order Details

Donkey anti-Sheep IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
Sheep IgG whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Sheep IgG and Sheep Serum.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:3000 - 1:15000
  • ELISA 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
Readout System

Reactivity Notes

Reacts with Sheep.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 40,000, Restore with 1.0 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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