Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed)

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Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP1-73718] - Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase donkey secondary antibody ...read more
Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP1-73718] - Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase donkey secondary antibody ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP
Format
Pre-absorbed
Concentration
LYOPH

Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed) Summary

Immunogen
Rabbit IgG whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • ELISA 1:10000 - 1:150000
  • Immunohistochemistry 1:500 - 1:2500
Readout System
Reviewed Applications
Read 1 Review rated 3
using
NBP1-73718 in the following applications:

Reactivity Notes

Reacts with Rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 40,000, Restore with 1.0 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP1-73718) (0)

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Review for IgG (H+L) Antibody (NBP1-73718) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-73718:
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Images Ratings Applications Species Date Details
 
reviewed by:
Sharon Miksys
WB Other 03/11/2015
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Summary

ApplicationWestern Blot
Sample TestedLiver, Brain
SpeciesOther

Blocking

Blocking Details5% (w/v) skim milk, 0.1% (w/v) BSA, 1% (v/v) normal horse serum, 2 hours, room temperature

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit IgG, H/L Chains Antibody [HRP]
Secondary Manufacturer Cat#NBP1-73718
Secondary Concentration1:5,000 to 1:15,000

Details

Detection NotesECL, 30 seconds to 5 minutes exposure

Comments

CommentsIt gave no unspecific signal when used to probe liver and brain samples in absence of primary antibodies. However, on the same samples previously probed with polyclonal primary antibody, the signal was very weak when compared to other anti-rabbit antibodies (NBP1-75290 and NBP1-75318) used at the same dilutions.

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Sharon Miksys
03/11/2015
Application: WB
Species: Other