Rabbit anti-Goat IgG (H+L) Secondary Antibody [Unconjugated]

Images

 
Western Blot: Rabbit anti-Goat IgG (H+L) Secondary Antibody [NBP1-72707] - Lane 1: Goat IgG Load: 100 ng per lane Secondary antibody: NBP1-73222 incubated for 60 min at RT; incubated in blocking buffer for 30 min at RT. ...read more
Immunocytochemistry/ Immunofluorescence: Rabbit anti-Goat IgG (H+L) Secondary Antibody [NBP1-72707] - Immunocytochemistry/ Immunofluorescence: Rabbit anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) ...read more
Western Blot: Rabbit anti-Goat IgG (H+L) Secondary Antibody [NBP1-72707] - Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Goat antibody at 1,000 overnight at 4C. Secondary antibody: DyLight 649 ...read more
Immunocytochemistry/ Immunofluorescence: Rabbit anti-Goat IgG (H+L) Secondary Antibody [NBP1-72707] - Analysis of huma liver cells using rabbit anti-goat IgG secondary antibody conjugated with DyLight 488. Image from ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2.1 mg/ml

Rabbit anti-Goat IgG (H+L) Secondary Antibody [Unconjugated] Summary

Immunogen
Goat IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:10000 - 1:60000
  • ELISA 1:800000
  • Immunohistochemistry 1:1000 - 1:5000
Application Notes
This product is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
2.1 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Additional IgG (H+L) Products

IgG (H+L) NBP1-72707

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