Goat anti-Human IgG (H+L) Secondary Antibody [DyLight 800] (Pre-absorbed)

Images

 
Western Blot: Goat anti-Human IgG (H+L) Secondary Antibody [DyLight 800] [NBP1-72869] - DyLigh t(R) dyes can be used for two-color Western Blot detection with low background and high signal.
Fluorophore-linked immunosorbent assay: Goat anti-Human IgG (H+L) Secondary Antibody [DyLight 800] [NBP1-72869]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, FLISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
DyLight 800
Format
Pre-absorbed
Concentration
LYOPH

Goat anti-Human IgG (H+L) Secondary Antibody [DyLight 800] (Pre-absorbed) Summary

Immunogen
Human IgG, whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Human IgG and with light chains of most Human immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Publications
Read Publication using
NBP1-72869 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 794, Excitation Wavelength: 770, Label Molecular Weight: 1,050, Restore with 0.1 mL of deionized water (or equivalent).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP1-72869)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for IgG (H+L) Antibody (NBP1-72869) (0)

There are no reviews for IgG (H+L) Antibody (NBP1-72869). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-72869) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG (H+L) Products

Blogs on IgG (H+L)

There are no specific blogs for IgG (H+L), but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Goat anti-Human IgG (H L) Secondary Antibody [DyLight 800] (Pre-absorbed) and receive a gift card or discount.