IgG (H+L) Antibodies
Immunoglobulin G (IgG), expressed on the surface of mature B cells, is often depicted as a Y-shape containing 2 heavy chains (H) and 2 light chains (L) linked by disulfide bonds. Browse our extensive catalog for polyclonal antibodies and IgG (H+L) conjugated secondary antibodies including FITC, HRP, and Biotin. Validated research applications include ChIP, ELISA, ICC, Immunofluorescence, IHC, IHC-Paraffin, IHC-Frozen, Flow Cytometry, IP, and Western Blot. Our IgG (H+L) secondary antibodies can be used in a variety of model species including Human, Mouse, Rat, Rabbit, Bovine, Canine, Chicken, Donkey, Equine, Feline, Goat, Llama, Porcine, and Sheep. Choose the anti-IgG (H+L) antibody best suited for your research needs by viewing the technical details, images, references, reviews and related products. Each IgG (H+L) antibody is fully covered by our Guarantee+.
Alternate Names for IgG (H+L) Antibodies
300 Results for "igg-(h-l)" in Secondary Antibodies
![Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of PP4C in Huh4 cell lysate. Image from verified customer review. Image using the HRP format of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-75286-img0009.jpg "Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of PP4C in Huh4 cell lysate. Image from verified customer review. Image using the HRP format of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of human osteosarcoma cell using Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75286-img0006.jpg)
![Immunohistochemistry-Frozen: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of frozen mouse tissue using DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-Frozen-NBP1-75286-img0008.jpg)
![Flow Cytometry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - analysis of LM7 cells using Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) conjugated to DyLight 594. Image from verified customer review. Image using the DyLight 594 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Flow-Cytometry-NBP1-75286-img0007.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - Analysis using Donkey anti-Goat IgG (H+L) Secondary Antibody using Embryo. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Western-Blot-NB7354-img0005.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - Analysis using Donkey anti-Goat IgG (H+L) Secondary Antibody using Embryo. Image using the HRP form of this antibody.")
![Immunohistochemistry-Paraffin: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:5,000 (0.2ug/ml). Secondary Antibody: Donkey anti-Goat IgG (H+L) Secondary Antibody HRP Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Immunohistochemistry-Paraffin-NB7354-img0004.jpg)
![Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB7567] - Analysis of Jurkat cell lysate using anti-MBD1 antibody (Cat. # NB100-56537) followed by HRP conjugated Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NB7567-img0003.jpg "Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB7567] - Analysis of Jurkat cell lysate using anti-MBD1 antibody (Cat. # NB100-56537) followed by HRP conjugated Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the HRP form of this antibody.")
![Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB120-6826] - Lane 1: Rabbit IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine chicken secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Chicken-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6831-img0002.jpg "Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB120-6826] - Lane 1: Rabbit IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine chicken secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa for Rabbit IgG.")
![Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Lane 1: Rabbit IgG. Load: 50 ug per lane. Secondary antibody: Texas Red (TM) chicken secondary antibody at 1:1,000 for 60 min at RT. Block for30 min at RT. Predicted/Observed size: 55 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Chicken-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6831-img0006.jpg)
![Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (CHICKEN) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Chicken-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6831-img0004.jpg)
![Dot Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Dot Blot of Chicken anti-Rabbit IgG (H+L) Secondary Antibody [Fluorescein] (Pre-absorbed). Antigen: Rabbit IgG Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Incubated for 1hr at RT; blocked for 60 min at RT. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Chicken-anti-Rabbit-IgG-H+L-Secondary-Antibody-Dot-Blot-NB120-6831-img0005.jpg)
![Western Blot: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Lane 1: Mouse IgG. Lane 2: None. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase sheep secondary antibody at 1:1,000 for 60 min at RT. Block for30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Image from the HRP version of this antibody.](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6710-img0012.jpg "Western Blot: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Lane 1: Mouse IgG. Lane 2: None. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase sheep secondary antibody at 1:1,000 for 60 min at RT. Block for30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Image from the HRP version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Immunocytochemistry/Immunofluorescence: Sheep anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72924]](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB120-6710-img0009.jpg)
![Western Blot: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein sheep secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6710-img0004.jpg)
![Dot Blot: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Mouse IgG. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Dot-Blot-NB120-6710-img0001.jpg)
![Fluorophore-linked immunosorbent assay: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Fluorophore-linked immunosorbent assay: Sheep anti-Mouse IgG, H/L Chains Antibody [DyLight 405] [NBP1-72929]](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-6710-img0002.jpg)
![ELISA: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - ELISA results of purified Sheep anti-Mouse IgG (H+L) Secondary antibody tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 ug of Mouse IgG. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Dky-anti-Sheep HRP conjugate, and TMB substrate.](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-ELISA-NB120-6710-img0014.jpg)
![Western Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Golden Syrian Hamster IgG (Lane 2) and Armenian Hamster IgG (Lane 3) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 5% Blotto 30 min at 20C, Antibody was used at 1:5000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-71730-img0003.jpg "Western Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Golden Syrian Hamster IgG (Lane 2) and Armenian Hamster IgG (Lane 3) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 5% Blotto 30 min at 20C, Antibody was used at 1:5000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - This image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).. Using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-71730-img0006.jpg)
![Western Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Anti-TNF-alpha was detected using a DyLight 800 conjugate. The image was captured using the Odyssey Infrared Imaging System developed by LI-COR.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-71730-img0002.jpg)
![ELISA: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - ELISA results of purified Goat anti-Hamster IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed) tested against purified Golden Syrian Hamster IgG and Armenian Hamster IgG. Each well was coated in duplicate with 1.0 ug of protein from different species. Golden Syrian Hamster IgG (Green Line), Armenian Hamster IgG (Red Line), Mouse IgG (Blue Line), and Rat IgG (Purple Line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-71730-img0004.jpg)
![Dot Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Dot Blot for Goat anti-Hamster IgG (H+L) Secondary Antibody [Texas Red] (Pre-adsorbed). Lane 1: 100 ng Hamster IgG. Lanes 2-5: serial dilution 3 fold of Antigen. Primary Antibody: none.Secondary Antibody: Goat anti-Hamster IgG (H+L) Secondary Antibody [Texas Red] (Pre-adsorbed) at 1:1000 dilution at RT for 1 hour. Block: Fluorescent blocking buffer at RT for 30 minutes.. Using the Texas Red format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NBP1-71730-img0005.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-71730-img0008.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75321] - Total cell lysate from human UMUC1. This image was submitted via customer Review. Image from the HRP version of Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-75321-img0004.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75321] - Total cell lysate from human UMUC1. This image was submitted via customer Review. Image from the HRP version of Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).")
![Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG (H+L) Secondary Antibody [NB7475] - This Goat anti-Human IgG (H+L) Secondary Antibody conjugated with pHAb Dyes which have excitation maxima (Ex ) at 532nm and emission maxima (Em) at 560nm. Used this with primary antibody of interest to visualize cells and study Internalisation using microscope. This image was submitted via customer review.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgG-H+L-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB7475-img0005.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG (H+L) Secondary Antibody [NB7475] - This Goat anti-Human IgG (H+L) Secondary Antibody conjugated with pHAb Dyes which have excitation maxima (Ex ) at 532nm and emission maxima (Em) at 560nm. Used this with primary antibody of interest to visualize cells and study Internalisation using microscope. This image was submitted via customer review.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG (H+L) Secondary Antibody [NB7475] - Human SKCO1 cells. Image from verified customer review. Image using the FITC form of Goat anti-Human IgG (H+L) Secondary Antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgG-H+L-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB7475-img0006.jpg)
