Goat anti-Human IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed)

Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed) [NBP1-74956] - Goat anti-Human IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed) gives very strong signal with low background when staining live cells (human cancer cell line) with humanized primary antibody. Image from verified customer review.

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: FITC
• Format: Pre-adsorbed
• Concentration: 1.5 mg/ml

Goat anti-Human IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed) Summary

• Description: Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
• Immunogen: This Goat anti-Human IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed) was developed against purified human IgG (H&L).
• Specificity: Based on IEP, this Goat anti-Human IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed) heavy gamma chains on human IgG and light chains on all human immunoglobulins. This antibody has been pre-adsorbed against bovine, goat, mouse or rabbit serum proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:20-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunomicroscopy 1:20 - 1:2000

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins and serum proteins from bovine, goat, mouse, or rabbit

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride (pH 7.2), 1% BSA, Protease/IgG free
• Preservative: 0.05% Sodium Azide
• Concentration: 1.5 mg/ml
• Purity: Affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Review for IgG (H+L) Antibody (NBP1-74956) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 10/31/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: Human cancer cell line
• Species: Human
• Lot: 27-78-120312