Rabbit anti-Mouse IgG (H L) Secondary Antibody

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Western Blot: Rabbit anti-Mouse IgG (H+L) Secondary Antibody [NBP1-72731] - Western Blot: Rabbit anti-Mouse IgG Secondary Antibody [Rhodamine] [NB120-6725] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane.
ELISA: Rabbit anti-Mouse IgG (H+L) Secondary Antibody [NBP1-72731] - ELISA results of purified Rabbit anti-Mouse IgG Antibody (min x Human Serum Proteins) tested against purified Mouse IgG. Each well was coated in ...read more
Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG (H+L) Secondary Antibody [NBP1-72731]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2 mg/ml

Rabbit anti-Mouse IgG (H L) Secondary Antibody Summary

Immunogen
Mouse IgG whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis (IEP) resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.No reaction was observed against Human Serum Proteins.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
Publications
Read Publication using
NBP1-72731 in the following applications:

  • WB
    1 publication

Reactivity Notes

Reacts with Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
2 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP1-72731)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol

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