Goat anti-Hamster IgG (H+L) Secondary Antibody [DyLight 800]

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Western Blot: Goat anti-Golden Syrian/Armenian Hamster IgG, H/L Chains Antibody [DyLight 800] [NBP1-73012] - Golden Syrian Hamster IgG (Lane 2) and Armenian Hamster IgG (Lane 3) was run on a 4-20% gel and transferred to ...read more
Fluorophore-linked immunosorbent assay: Goat anti-Golden Syrian/Armenian Hamster IgG, H/L Chains Antibody [DyLight 800] [NBP1-73012]

Product Details

Summary
Reactivity HaSpecies Glossary
Applications WB, FLISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
DyLight 800
Concentration
LYOPH

Goat anti-Hamster IgG (H+L) Secondary Antibody [DyLight 800] Summary

Immunogen
Golden Syrian & Armenian Hamster IgG, whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian & Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Golden Syrian & Armenian Hamster and Golden Syrian & Armenian Hamster Serum. No reaction was observed against Mouse or Rat Serum Proteins. This antibody will react with heavy chains of Golden Syrian & Armenian Hamster IgG and with light chains of most Golden Syrian & Armenian Hamster immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:25000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Reactivity Notes

Reacts with Golden Syrian and Armenian Hamster.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 794, Excitation Wavelength: 770, Label Molecular Weight: 1,050, Restore with 0.1 mL of deionized water (or equivalent).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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