Rabbit anti-Mouse IgG (H+L) Secondary Antibody [Biotin]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin
Concentration
2.0 mg/ml

Rabbit anti-Mouse IgG (H L) Secondary Antibody [Biotin] Summary

Immunogen
Full length native Mouse IgG (purified).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Application Notes
Dot: Use at an assay dependant dilution. ELISA: Use at a dilution of 1/20000 - 1/80000. IHC-P: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. IM: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Readout System

Reactivity Notes

Minimum Cross Reactivity to Human Serum Proteins

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin
Preservative
0.05% Sodium Azide
Concentration
2.0 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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