Rabbit anti-Mouse IgG (H+L) Secondary Antibody [HRP]

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Western Blot: Rabbit anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NBP1-73435] - WB shows detection of mouse anti-beta Actin monoclonal antibody (Catalog# NBP1-47423) using a HRP conjugated rabbit anti-mouse IgG H/L ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP
Concentration
LYOPH

Rabbit anti-Mouse IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
Mouse IgG whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG, and Mouse Serum. No reaction was observed against Human Serum Proteins. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2500
  • ELISA 1:5000 - 1:20000
  • Immunohistochemistry 1:200 - 1:1000
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NBP1-73435 in the following applications:

Reactivity Notes

Reacts with Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 40,000, Restore with 1.0 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Review for IgG (H+L) Antibody (NBP1-73435) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-73435:
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Images Ratings Applications Species Date Details
Western Blot IgG (H+L) NBP1-73435
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reviewed by:
Anonymous
WB Human 10/07/2015
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Summary

ApplicationWestern Blot
Sample Testedhuman cancer cell lysate
SpeciesHuman
Lot32411

Blocking

Blocking Details5% non-fat milk in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:3000 in 5% non-fat milk in TBS buffer overnight at 4C

Secondary Antibody

Secondary DescriptionMouse IgG, H/L Chains Antibody (NBP1-73435)
Secondary Manufacturer Cat#NBP1-73435
Secondary Concentration1:5000

Details

Detection Notes15-second exposure shows strong band at correct MW.

Comments

CommentsThis secondary antibody works very well. In my test, it is specific to mouse primary only and does not react with rabbit antibody.

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Anonymous
10/07/2015
Application: WB
Species: Human