Goat anti-Mouse IgG (H+L) Secondary Antibody [Janelia Fluor 549] (Pre-adsorbed)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Janelia Fluor 549
Format
Pre-adsorbed

Order Details

Goat anti-Mouse IgG (H+L) Secondary Antibody [Janelia Fluor 549] (Pre-adsorbed) Summary

Immunogen
This Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against mouse IgG whole molecule.
Specificity
Assay of Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat serum, Mouse IgG and Mouse serum. No reaction was observed against Human serum proteins. Specificity was confirmed using ELISA at less than 1% cross reactivity against Human IgG. This antibody was pre-adsorbed against human serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-72739JF549) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Alkaline Phosphatase NBP1-72669
Allophycocyanin/Cy7 NBP1-72739APCCY7
Biotin NB7605
DyLight 405 NBP1-72739V
DyLight 488 NBP1-72874
DyLight 550 NBP1-72739R
DyLight 594 NBP1-72739DL594
DyLight 650 NBP1-72739C
DyLight 680 NBP1-72882
DyLight 755 NBP1-72739IR
FITC NBP1-71769
HRP NBP1-73322
Janelia Fluor 549 NBP1-72739JF549
Janelia Fluor 646 NBP1-72739JF646
PE NB7594
PE/Atto594 NBP1-72739PEATT594
PE/Cy5.5 NBP1-72739PECY55
PE/Cy7 NBP1-72739PECY7
Rhodamine NB120-7065
Rhodamine NBP1-73133
Texas Red NBP1-73623

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