Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488]

Images

 
Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72889] - Hep core expression in Huh7 cells. Cells were fixed in 4% paraformaldehyde for 20 minutes at room ...read more
Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72889]
Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72889]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, FLISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 488
Concentration
LYOPH

Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] Summary

Immunogen
Mouse IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-72889 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 100 ul of deionized water.

Notes

Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mouse IgG, H/L Chains Antibody (NBP1-72889) (0)

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Review for Mouse IgG, H/L Chains Antibody (NBP1-72889) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-72889:
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IF
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence IgG (H+L) NBP1-72889
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reviewed by:
Anonymous
IF Human 11/12/2014
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Summary

ApplicationImmunofluorescence
Sample TestedHUH7
SpeciesHuman

Blocking

Blocking Detailsblocked in 1% BSA in PBS, 20 mins at RT

Primary Anitbody

Dilution Ratioprimary antibody against viral protein, diluted 1/500 in 1%BSA, incubated 20 min RT

Secondary Antibody

Secondary Descriptionthis is the secondary
Secondary Concentration1/500 in 1% BSA in PBS, 20 mins at RT

Details

Detection Notesimmunofluorescence
Fixation Detailsfixed in 4% paraformaldehyde, 20 mins at RT
Wash Descriptionwashed three times between each incubation with PBS

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mouse IgG, H/L Chains Antibody (NBP1-72889) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG (H+L) Products

IgG (H+L) NBP1-72889

Blogs on IgG (H+L)

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Anonymous
11/12/2014
Application: IF
Species: Human