Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG, H/L Chains Antibody [DyLight 488] [NBP1-72889] - Hep core expression in Huh7 cells. Cells were fixed in 4% paraformaldehyde for 20 minutes at room ...read more
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins. This antibody will react with heavy chains of Mouse IgG and with light chains of most Mouse immunoglobulins.
Immunogen affinity purified
Western Blot 1:10000
Fluorophore-linked immunosorbent assay 1:20000
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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