Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-adsorbed)

Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72889] - Hep core expression in Huh7 cells. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature. The secondary antibody was diluted in 1% BSA in PBS at 1:500 dilution for 20 minutes at room temperature. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72889]

Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72889]

• Reactivity: Mu
• Applications: WB, FLISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 488
• Format: Pre-adsorbed
• Concentration: LYOPH

Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-adsorbed) Summary

• Immunogen: Mouse IgG whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:10000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
• Application Notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 100 ul of deionized water.

Notes

Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Review for Mouse IgG, H/L Chains Antibody (NBP1-72889) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous IF Human 11/12/2014

Summary

• Application: Immunofluorescence
• Sample Tested: HUH7
• Species: Human

Blocking

• Blocking Details: blocked in 1% BSA in PBS, 20 mins at RT

Primary Anitbody

• Dilution Ratio: primary antibody against viral protein, diluted 1/500 in 1%BSA, incubated 20 min RT

Secondary Antibody

• Secondary Description: this is the secondary
• Secondary Concentration: 1/500 in 1% BSA in PBS, 20 mins at RT

Details

• Detection Notes: immunofluorescence
• Fixation Details: fixed in 4% paraformaldehyde, 20 mins at RT
• Wash Description: washed three times between each incubation with PBS