Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488]


Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG, H/L Chains Antibody [DyLight 488] [NBP1-72889] - Hep core expression in Huh7 cells. Cells were fixed in 4% paraformaldehyde for 20 minutes at room ...read more
Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG, H/L Chains Antibody [DyLight 488] [NBP1-72889]

Product Details

Reactivity MuSpecies Glossary
Applications WB, FLISA, ICC/IF
LYOPH mg/ml

Order Details

Rabbit anti-Mouse IgG (H L) Secondary Antibody [DyLight 488] Summary

Mouse IgG, whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins. This antibody will react with heavy chains of Mouse IgG and with light chains of most Mouse immunoglobulins.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
DyLight 488
DyLight 488 (A=493, E=518)
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
0.01% Sodium Azide
LYOPH mg/ml
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.


Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reviewed Applications
Read 1 Review rated 5
NBP1-72889 in the following application:

Reactivity Notes

Reacts with Mouse.


Emission Wavelength: 518, Excitation Wavelength: 493, Label Molecular Weight: 1,011, Restore with 0.1 mL of deionized water (or equivalent).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mouse IgG, H/L Chains Antibody (NBP1-72889) (0)

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Reviews for Mouse IgG, H/L Chains Antibody (NBP1-72889) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-72889:
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Images Ratings Applications Species Date Details
 IgG (H+L) NBP1-72889
reviewed by:
Human 11/12/2014


Sample TestedHUH7


Blocking Detailsblocked in 1% BSA in PBS, 20 mins at RT

Primary Anitbody

Dilution Ratioprimary antibody against viral protein, diluted 1/500 in 1%BSA, incubated 20 min RT

Secondary Antibody

Secondary Descriptionthis is the secondary
Secondary Concentration1/500 in 1% BSA in PBS, 20 mins at RT


Detection Notesimmunofluorescence
Fixation Detailsfixed in 4% paraformaldehyde, 20 mins at RT
Wash Descriptionwashed three times between each incubation with PBS

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mouse IgG, H/L Chains Antibody (NBP1-72889) (0)

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