Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488]

Images

 
Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG, H/L Chains Antibody [DyLight 488] [NBP1-72889] - Hep core expression in Huh7 cells. Cells were fixed in 4% paraformaldehyde for 20 minutes at room ...read more
Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG, H/L Chains Antibody [DyLight 488] [NBP1-72889]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, FLISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 488
Concentration
LYOPH

Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] Summary

Immunogen
Mouse IgG, whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins. This antibody will react with heavy chains of Mouse IgG and with light chains of most Mouse immunoglobulins.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-72889 in the following applications:

Reactivity Notes

Reacts with Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 518, Excitation Wavelength: 493, Label Molecular Weight: 1,011, Restore with 0.1 mL of deionized water (or equivalent).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Mouse IgG, H/L Chains Antibody (NBP1-72889) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NBP1-72889:
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IF
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Images Ratings Applications Species Date Details
Immunofluorescence IgG (H+L) NBP1-72889
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reviewed by:
Anonymous
IF Human 11/12/2014
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Summary

ApplicationImmunofluorescence
Sample TestedHUH7
SpeciesHuman

Blocking

Blocking Detailsblocked in 1% BSA in PBS, 20 mins at RT

Primary Anitbody

Dilution Ratioprimary antibody against viral protein, diluted 1/500 in 1%BSA, incubated 20 min RT

Secondary Antibody

Secondary Descriptionthis is the secondary
Secondary Concentration1/500 in 1% BSA in PBS, 20 mins at RT

Details

Detection Notesimmunofluorescence
Fixation Detailsfixed in 4% paraformaldehyde, 20 mins at RT
Wash Descriptionwashed three times between each incubation with PBS

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mouse IgG, H/L Chains Antibody (NBP1-72889) (0)

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Anonymous
11/12/2014
Application: IF
Species: Human