Rabbit anti-Sheep IgG (H+L) Secondary Antibody [DyLight 800]


Fluorophore-linked immunosorbent assay: Rabbit anti-Sheep IgG, H/L chains Antibody [DyLight 800] [NBP1-73000]

Product Details

Reactivity ShSpecies Glossary
Applications WB, FLISA, ICC/IF
LYOPH mg/ml

Order Details

Rabbit anti-Sheep IgG (H L) Secondary Antibody [DyLight 800] Summary

Sheep IgG, whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG and Sheep Serum. This antibody will react with heavy chains of Sheep IgG and with light chains of most Sheep immunoglobulins.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
DyLight 800
DyLight 800 (A=777, E=794)
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
0.01% Sodium Azide
LYOPH mg/ml
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.


  • Western Blot 1:10000-1:25000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Reactivity Notes

Reacts with Sheep.


Emission Wavelength: 794, Excitation Wavelength: 770, Label Molecular Weight: 1,050, Restore with 0.1 mL of deionized water (or equivalent). Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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