Goat anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed)

Images

 
Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72874] - 100 ug of Mouse IgG (Lane 2) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with ...read more
Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72874] - DyLight and ATTO dye conjugated antibodies provide high signal and low background for ...read more
Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72874] - Staining of paraffin embedded mouse tissue. Goat anti-Mouse DyLight 488 was used as a secondary ...read more
Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72874] - DyLight and ATTO dye conjugated antibodies provide high signal and low background for ...read more
Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72874] - MDA-MB-231 cells were stained. Goat anti-Mouse DyLight 488 was used as a secondary ...read more
Immunohistochemistry-Frozen: Goat anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72874] - Staining of mouse frozen tissue. Goat anti-Mouse DyLight 488 was used as a secondary antibody. Image ...read more
Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72874]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, FLISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
DyLight 488
Format
Pre-absorbed
Concentration
LYOPH

Goat anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) Summary

Immunogen
Mouse IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reviewed Applications
Read 4 Reviews rated 4
using
NBP1-72874 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 100 ul of deionized water.

Notes

Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Reviews for IgG (H+L) Antibody (NBP1-72874) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-72874:
Filter by Applications
IHC-P
(1)
IHC-Fr
(1)
ICC
(1)
Flow
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin IgG (H+L) NBP1-72874
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reviewed by:
Arun Satelli
IHC-P Mouse 11/04/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse tissue
SpeciesMouse
CommentsNot as good as that on the frozen section.

Blocking

Blocking DetailsFish Gelatin

Details

Detection NotesDetection using fluoroscent microscope
Fixation DetailsRegular fixation of IHC paraffin tissue
Wash Description5 min each 3 times

Comments

CommentsNot as good as that on the frozen section.
Immunohistochemistry-Frozen IgG (H+L) NBP1-72874
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reviewed by:
Arun Satelli
IHC-Fr Mouse 11/04/2014
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse tissue
SpeciesMouse
CommentsShowed less background and is good too.

Blocking

Blocking DetailsFish Gelatin

Primary Anitbody

Dilution Ratio1:200

Details

Detection NotesDetection using fluoroscent microscope
Fixation DetailsRegular fixation of IHC frozen sections

Comments

CommentsShowed less background and is good too.
Immunocytochemistry IgG (H+L) NBP1-72874
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reviewed by:
Arun Satelli
ICC Human 11/04/2014
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Summary

ApplicationImmunocytochemistry
Sample TestedMDA MB 231 cells
SpeciesHuman
CommentsVery sensitive antibody. However observed bleaching in some areas, but digitalization still good.

Blocking

Blocking Details0.25% FBS in PBS

Primary Anitbody

Dilution Ratio1:250

Details

Detection NotesDetection using confocal microscope LSM510
Fixation DetailsCells were fixed in 4% PFA, PBS, Overnight 4 deg C

Comments

CommentsVery sensitive antibody. However observed bleaching in some areas, but digitalization still good.
Flow Cytometry IgG (H+L) NBP1-72874
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reviewed by:
Arun Satelli
Flow Human 10/23/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman osteosarcoma cell line
SpeciesHuman

Blocking

Blocking Details0.25% FBS in PBS

Primary Anitbody

Dilution Ratio1:200

Details

Detection NotesDetection using flow cytometer
Fixation DetailsFixing using 4% PFA, Block in 0.25% FBS,
Permeabilization Detailsno permeabilization
Wash Description5 min each 3 times in PBS

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-72874) (0)

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Recent Reviews

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Arun Satelli
11/04/2014
Application: IHC-P
Species: Mouse

Arun Satelli
11/04/2014
Application: IHC-Fr
Species: Mouse

Arun Satelli
11/04/2014
Application: ICC
Species: Human