| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94903-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26271-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85586-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87783-25ul | |||
![Western Blot: RNF166 Antibody [NBP2-94903] - Analysis of extracts of various cell lines, using RNF166 . Exposure time: 15s.](http://images.novusbio.com/fullsize/RNF166-Antibody-Western-Blot-NBP2-94903-img0005.jpg "Western Blot: RNF166 Antibody [NBP2-94903] - Analysis of extracts of various cell lines, using RNF166 . Exposure time: 15s.")
![Immunohistochemistry-Paraffin: RNF166 Antibody [NBP2-94903] - Paraffin-embedded rat kidney using RNF166 .](http://images.novusbio.com/fullsize/RNF166-Antibody-Immunohistochemistry-Paraffin-NBP2-94903-img0004.jpg)
![Immunohistochemistry-Paraffin: RNF166 Antibody [NBP2-94903] - Paraffin-embedded human liver damage using RNF166 .](http://images.novusbio.com/fullsize/RNF166-Antibody-Immunohistochemistry-Paraffin-NBP2-94903-img0001.jpg)
![Immunohistochemistry-Paraffin: RNF166 Antibody [NBP2-94903] - Paraffin-embedded mouse brain using RNF166 .](http://images.novusbio.com/fullsize/RNF166-Antibody-Immunohistochemistry-Paraffin-NBP2-94903-img0002.jpg)
![Immunohistochemistry-Paraffin: RNF166 Antibody [NBP2-94903] - Paraffin-embedded rat brain using RNF166 .](http://images.novusbio.com/fullsize/RNF166-Antibody-Immunohistochemistry-Paraffin-NBP2-94903-img0003.jpg)
![Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] - Western blot analysis of lysates from wild type(WT) and TRAF-2 knockdown (KD) 293T cells, using [KD Validated] TRAF-2 Rabbit mAb at 1:50000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15682_rabbit-traf-2-mab-3s2a8-522025173453.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] - Western blot analysis of lysates from wild type(WT) and TRAF-2 knockdown (KD) 293T cells, using [KD Validated] TRAF-2 Rabbit mAb at 1:50000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] - Western blot analysis of various lysates, using [KD Validated] TRAF-2 Rabbit mAb at 1:50000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15682_rabbit-traf-2-mab-3s2a8-52202517304918.jpg)
![Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] - Western blot analysis of various lysates, using [KD Validated] TRAF-2 Rabbit mAb at 1:50000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15682_rabbit-traf-2-mab-3s2a8-5220251736129.jpg)
![Immunoprecipitation: TRAF-2 Antibody (3S2A8) [TRAF-2] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug TRAF-2 antibody . Western blot was performed from the immunoprecipitate using TRAF-2 antibody at a dilution of 1:50000.](http://images.novusbio.com/fullsize/nbp3-15682_rabbit-traf-2-mab-3s2a8-52202517361223.jpg)
![Immunoprecipitation: TRAF-2 Antibody (9A5) [NBP3-26271] - Immunoprecipitating TRAF-2 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26271 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26271 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26271_rabbit-traf-2-mab-9a5-26220241322157.jpg "Immunoprecipitation: TRAF-2 Antibody (9A5) [NBP3-26271] - Immunoprecipitating TRAF-2 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26271 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26271 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Immunohistochemistry: TRAF-2 Antibody (9A5) [NBP3-26271] - Image of TRAF-2 Antibody (9A5) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26271_rabbit-traf-2-mab-9a5-262202414471589.jpg)
![Immunohistochemistry: TRAF-2 Antibody (9A5) [NBP3-26271] - Image of TRAF-2 Antibody (9A5) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26271_rabbit-traf-2-mab-9a5-262202415355732.jpg)
![Western Blot: TRAF-2 Antibody (9A5) [NBP3-26271] - Positive Western Blot detected in: HepG2 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, K562 whole cell lysate. All lanes: TRAF-2 Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56, 62, 55, 54 kDa Observed band size: 56 kDa](http://images.novusbio.com/fullsize/nbp3-26271_rabbit-traf-2-mab-9a5-28220241140929.jpg)
![Flow Cytometry: TRAF-2 Antibody (9A5) [NBP3-26271] - Overlay histogram showing Hela cells stained with TRAF-2 Antibody (9A5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26271_rabbit-traf-2-mab-9a5-282202414183692.jpg)
![Western Blot: RNF36 Antibody [NB100-1207] - (0.5ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/RNF36-Antibody-Western-Blot-NB100-1207-img0004.jpg "Western Blot: RNF36 Antibody [NB100-1207] - (0.5ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207] - Staining of paraffin embedded Human Cerebellum at dilution of 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/RNF36-Antibody-Immunohistochemistry-Paraffin-NB100-1207-img0005.jpg)
![Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/RNF36-Antibody-Immunohistochemistry-Paraffin-NB100-1207-img0003.jpg)
![Western Blot: SH3MD2 Antibody (3H3) [H00057630-M01] - SH3MD2 monoclonal antibody (M01), clone 3H3 Analysis of SH3MD2 expression in HeLa.](http://images.novusbio.com/fullsize/SH3MD2-Antibody-3H3-Western-Blot-H00057630-M01-img0005.jpg "Western Blot: SH3MD2 Antibody (3H3) [H00057630-M01] - SH3MD2 monoclonal antibody (M01), clone 3H3 Analysis of SH3MD2 expression in HeLa.")
![Western Blot: SH3MD2 Antibody (3H3) [H00057630-M01] - SH3MD2 monoclonal antibody (M01), clone 3H3. Analysis of SH3MD2 expression in PC-12.](http://images.novusbio.com/fullsize/SH3MD2-Antibody-3H3-Western-Blot-H00057630-M01-img0006.jpg)
![ELISA: SH3MD2 Antibody (3H3) [H00057630-M01] - Detection limit for recombinant GST tagged SH3MD2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SH3MD2-Antibody-3H3-ELISA-H00057630-M01-img0007.jpg)
![Western Blot: RNF111 Antibody [H00054778-B01P] - Analysis of RNF111 expression in rat brain.](http://images.novusbio.com/fullsize/RNF111-Antibody-Western-Blot-H00054778-B01P-img0005.jpg "Western Blot: RNF111 Antibody [H00054778-B01P] - Analysis of RNF111 expression in rat brain.")
![Immunohistochemistry-Paraffin: RNF111 Antibody [H00054778-B01P] - Analysis of purified antibody to RNF111 on formalin-fixed paraffin-embedded human prostate. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/RNF111-Antibody-Immunohistochemistry-Paraffin-H00054778-B01P-img0004.jpg)
![Western Blot: RNF111 Antibody [H00054778-B01P] - Analysis of RNF111 expression in transfected 293T cell line by RNF111 polyclonal antibody. Lane 1: RNF111 transfected lysate(108.35 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RNF111-Antibody-Western-Blot-H00054778-B01P-img0006.jpg)
![Western Blot: MKRN2 Antibody (5F8) [H00023609-M01] - MKRN2 monoclonal antibody (M01), clone 5F8 Analysis of MKRN2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/MKRN2-Antibody-5F8-Western-Blot-H00023609-M01-img0010.jpg "Western Blot: MKRN2 Antibody (5F8) [H00023609-M01] - MKRN2 monoclonal antibody (M01), clone 5F8 Analysis of MKRN2 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: MKRN2 Antibody (5F8) [H00023609-M01] - Analysis of monoclonal antibody to MKRN2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MKRN2-Antibody-5F8-Immunocytochemistry-Immunofluorescence-H00023609-M01-img0007.jpg)
![Western Blot: MKRN2 Antibody (5F8) [H00023609-M01] - Analysis of MKRN2 over-expressed 293 cell line, cotransfected with MKRN2 Validated Chimera RNAi ( Cat # H00023609-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MKRN2 monoclonal antibody (M01), clone 5F8 (Cat # H00023609-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/MKRN2-Antibody-5F8-Western-Blot-H00023609-M01-img0008.jpg)
![Western Blot: MKRN2 Antibody (5F8) [H00023609-M01] - Analysis of MKRN2 expression in transfected 293T cell line by MKRN2 monoclonal antibody (M01), clone 5F8.Lane 1: MKRN2 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MKRN2-Antibody-5F8-Western-Blot-H00023609-M01-img0011.jpg)
![Sandwich ELISA: MKRN2 Antibody (5F8) [H00023609-M01] - Detection limit for recombinant GST tagged MKRN2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MKRN2-Antibody-5F8-Sandwich-ELISA-H00023609-M01-img0009.jpg)
![Western Blot: RNF98 Antibody (2D11) [H00055521-M01] - TRIM36 monoclonal antibody (M01), clone 2D11 Analysis of TRIM36 expression in IMR-32.](http://images.novusbio.com/fullsize/RNF98-Antibody-2D11-Western-Blot-H00055521-M01-img0010.jpg "Western Blot: RNF98 Antibody (2D11) [H00055521-M01] - TRIM36 monoclonal antibody (M01), clone 2D11 Analysis of TRIM36 expression in IMR-32.")
![Immunocytochemistry/Immunofluorescence: RNF98 Antibody (2D11) [H00055521-M01] - Analysis of monoclonal antibody to TRIM36 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RNF98-Antibody-2D11-Immunocytochemistry-Immunofluorescence-H00055521-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: RNF98 Antibody (2D11) [H00055521-M01] - Analysis of monoclonal antibody to TRIM36 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RNF98-Antibody-2D11-Immunohistochemistry-Paraffin-H00055521-M01-img0008.jpg)
![Sandwich ELISA: RNF98 Antibody (2D11) [H00055521-M01] - Detection limit for recombinant GST tagged TRIM36 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RNF98-Antibody-2D11-Sandwich-ELISA-H00055521-M01-img0009.jpg)
![Immunohistochemistry: PML Protein Antibody (PSH02-89) [NBP3-32832] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PML Protein antibody (NBP3-32832) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32832) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32832_rabbit-pml-protein-mab-psh02-89-298202414115433.jpg "Immunohistochemistry: PML Protein Antibody (PSH02-89) [NBP3-32832] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PML Protein antibody (NBP3-32832) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32832) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: PML Protein Antibody (PSH02-89) [NBP3-32832] - Immunocytochemistry analysis of MDA-MB-231 cells labeling PML Protein with Rabbit anti-PML Protein antibody (NBP3-32832) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PML Protein antibody (NBP3-32832) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32832_rabbit-pml-protein-mab-psh02-89-29202410334037.jpg)
![Flow Cytometry: PML Protein Antibody (PSH02-89) [NBP3-32832] - Flow cytometric analysis of A431 cells labeling PML Protein. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32832, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32832_rabbit-pml-protein-mab-psh02-89-29202416453264.jpg)
![Western Blot: PML Protein Antibody (PSH02-89) [NBP3-32832] - Western blot analysis of PML Protein on different lysates with Rabbit anti-PML Protein antibody (NBP3-32832) at 1/2,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: K-562 cell lysate (15 µg/Lane) Lane 4: A549 cell lysate (15 µg/Lane) Lane 5: MDA-MB-231 cell lysate (15 µg/Lane) Lane 6: A431 cell lysate (15 µg/Lane) Predicted band size: 98 kDa Observed band size: 50-130 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32832_rabbit-pml-protein-mab-psh02-89-3920248453512.jpg)
![Immunocytochemistry/Immunofluorescence: ZNF364 Antibody [NBP1-85586] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ZNF364-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85586-img0007.jpg "Immunocytochemistry/Immunofluorescence: ZNF364 Antibody [NBP1-85586] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & mitochondria. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: ZNF364 Antibody [NBP1-85586] - Staining of human stomach shows strong cytoplasmic positivity in chief cells.](http://images.novusbio.com/fullsize/ZNF364-Antibody-Immunohistochemistry-Paraffin-NBP1-85586-img0002.jpg)
![Immunohistochemistry-Paraffin: RNF113A Antibody [NBP1-85378] - Staining of human duodenum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RNF113A-Antibody-Immunohistochemistry-Paraffin-NBP1-85378-img0006.jpg "Immunohistochemistry-Paraffin: RNF113A Antibody [NBP1-85378] - Staining of human duodenum shows strong nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: RNF113A Antibody [NBP1-85378] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes as expected.](http://images.novusbio.com/fullsize/RNF113A-Antibody-Immunohistochemistry-Paraffin-NBP1-85378-img0001.jpg)
![Immunohistochemistry-Paraffin: RNF113A Antibody [NBP1-85378] - Staining of human lymph node shows strong nuclear postivity in germinal center cells and non-germinal center cells.](http://images.novusbio.com/fullsize/RNF113A-Antibody-Immunohistochemistry-Paraffin-NBP1-85378-img0002.jpg)
![Immunohistochemistry-Paraffin: RNF113A Antibody [NBP1-85378] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/RNF113A-Antibody-Immunohistochemistry-Paraffin-NBP1-85378-img0003.jpg)
![Immunohistochemistry-Paraffin: RNF113A Antibody [NBP1-85378] - Staining of human liver shows moderate nuclear postivity in hepatocytes.](http://images.novusbio.com/fullsize/RNF113A-Antibody-Immunohistochemistry-Paraffin-NBP1-85378-img0004.jpg)
![Immunohistochemistry-Paraffin: RNF113A Antibody [NBP1-85378] - Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RNF113A-Antibody-Immunohistochemistry-Paraffin-NBP1-85378-img0005.jpg)
![Immunohistochemistry-Paraffin: PML Protein Antibody [NBP1-87783] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NBP1-87783-img0006.jpg "Immunohistochemistry-Paraffin: PML Protein Antibody [NBP1-87783] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.")
