SH3RF2 Antibody - BSA Free

Western Blot: SH3RF2 Antibody [NBP1-76284] - 3T3 cell lysate with SH3RF2 antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry/Immunofluorescence: SH3RF2 Antibody [NBP1-76284] - Human brain tissue with SH3RF2 antibody at 20 ug/mL.

Immunohistochemistry: SH3RF2 Antibody [NBP1-76284] - Human brain tissue with SH3RF2 antibody at 10 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SH3RF2 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SH3RF2. The immunogen is located within the last 50 amino acids of SH3RF2. Amino Acid Squence: CHSGQQTDLRRKSALGK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SH3RF2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  SH3RF2 Peptide (NBP1-76284PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SH3RF2 Antibody - BSA Free

• EC 6.3.2
• EC 6.3.2.-
• Heart protein phosphatase 1-binding protein
• HEPP1FLJ23654
• MGC149788
• MGC149789
• putative E3 ubiquitin-protein ligase SH3RF2
• RING finger protein 158
• RNF158MGC90410
• SH3 domain containing ring finger 2
• SH3 domain-containing RING finger protein 2

Background

Although the SH3 domain containing ring finger 2 protein (SH3RF2) has been identified as a marker of the nucleus accumbens in the human brain, little is known about this protein. Recent yeast two-hybrid experiments have shown that SH3RF2 associates with protein phosphatase 1 (PP1) and is expressed primarily in heart and testis tissue. Recombinant SH3RF2 enhanced PP1 enzymatic activity and antagonized the ability of phopho-inhibitor-1 or -2 to inhibit PP1, suggesting that SH3RF2 may play a role working with PP1 in cardiac functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SH3RF2
• Uniprot:
  • NP_689763(Human)