| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33138 | |||
| IHC-Prediluted | NBP2-44584 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-22131SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56462SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70509 | |||
![Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Western-Blot-NB300-749-img0007.jpg "Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).")
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 11) [NB300-749] - Mouse fibroblasts.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunocytochemistry-Immunofluorescence-NB300-749-img0004.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0006.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0005.jpg)
![Western Blot: CHD8 Antibody [NB100-60417] - Detection of Human and Mouse CHD8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody NB100-60417 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHD8-Antibody-Western-Blot-NB100-60417-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: CHD8 Antibody [NB100-60417] - Detection of Human and Mouse CHD8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody NB100-60417 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CHD8 Antibody [NB100-60417] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CHD8-Antibody-Immunohistochemistry-Paraffin-NB100-60417-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD8 Antibody [NB100-60417] - Detection of human CHD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody NB100-60417 used for IP at 6 ug/mg lysate. CHD8 was also immunoprecipitated by rabbit anti-CHD8 antibody NB100-60418. For blotting immunoprecipitated CHD8, NB100-60417 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CHD8-Antibody-Immunoprecipitation-NB100-60417-img0010.jpg)
![Western Blot: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - analysis of Cyclin D1 in 1) C2C12, 2) HepG2, and 3) NIH3T3 lysate probed with Cyclin D1 antibody at 5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Western-Blot-NBP2-15189-img0003.jpg "Western Blot: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - analysis of Cyclin D1 in 1) C2C12, 2) HepG2, and 3) NIH3T3 lysate probed with Cyclin D1 antibody at 5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Formalin-fixed, paraffin-embedded human Mantle Cell Lymphoma stained with Cyclin D1 Antibody (DCS-6).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Immunohistochemistry-Paraffin-NBP2-15189-img0010.jpg)
![Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Flow Cytometric analysis of human Cyclin D1 on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (DCS-6).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Flow-Cytometry-NBP2-15189-img0011.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Formalin-paraffin human mantle cell lymphoma stained with Cyclin D1 Ab (Clone DCS-6). Note nuclear staining of tumor cells.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Immunohistochemistry-Paraffin-NBP2-15189-img0006.jpg)
![Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Analysis using Alexa Fluor (R) 647 conjugate of NBP2-15189. Cyclin D1 staining of HCT116 cells using Alexa Fluor 647 conjugated Cyclin D1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Flow-Cytometry-NBP2-15189-img0008.jpg)
![Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Flow Cytometry of human Cyclin D1 on Jurkat cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Antibody (DCS-6).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Flow-Cytometry-NBP2-15189-img0009.jpg)
![Western Blot: CaMKII beta Antibody [NB120-22131] - Analysis of CAMKIIb in rat brain lysate using NB120-22131 at 1:2000.](http://images.novusbio.com/fullsize/CaMKII-beta-Antibody-Western-Blot-NB120-22131-img0002.jpg "Western Blot: CaMKII beta Antibody [NB120-22131] - Analysis of CAMKIIb in rat brain lysate using NB120-22131 at 1:2000.")
![Immunocytochemistry/Immunofluorescence: CaMKII beta Antibody [NB120-22131] - Staining of CAMKIIb using NB120-22131 in rat hippocampal neurons at a dilution of 1:100, note that staining is rather weak.](http://images.novusbio.com/fullsize/CaMKII-beta-Antibody-Immunocytochemistry-Immunofluorescence-NB120-22131-img0001.jpg)
![Western Blot: CaMKII beta Antibody [NB120-22131] - Analysis of CAMKII beta in A) human, B) mouse and C) rat brain lysate using CAMKII beta antibody at 1:2000.](http://images.novusbio.com/fullsize/CaMKII-beta-Antibody-Western-Blot-NB120-22131-img0003.jpg)
![Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Western-Blot-NB100-56462-img0004.jpg "Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.")
![Immunohistochemistry-Paraffin: Smad2 Antibody [NB100-56462] - Mouse testis tissue probed with this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Immunohistochemistry-Paraffin-NB100-56462-img0003.jpg)
![Western Blot: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Affinity Purified PPAR delta/NR1C2/NR1C2 antibody is shown to detect a predominant band at ~ 40kDa corresponding to PPAR delta/NR1C2 present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4 C followed reaction with a 1:10,000 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye(R) 800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody---N-terminal-Western-Blot-NB600-637-img0007.jpg "Western Blot: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Affinity Purified PPAR delta/NR1C2/NR1C2 antibody is shown to detect a predominant band at ~ 40kDa corresponding to PPAR delta/NR1C2 present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4 C followed reaction with a 1:10,000 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye(R) 800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Immunohistochemistry: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Immunohistochemistry of PPAR delta/NR1C2/NR1C2 antibody. Antigen Retrieval: HIER pH 6.2. [Right] -Neg Ctrl: Normal rabbit IgG on heart striated muscle, pH6.2 (40X). [Left Top - low mag, Left Bottom - 20X] -Staining: Diffuse cytoplasmatic positivity for PPAR delta/NR1C2 in human myocardiocitys at 1:500.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody---N-terminal-Immunohistochemistry-NB600-637-img0008.jpg)
![ELISA: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - ELISA results of purified PPAR delta/NR1C2 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody---N-terminal-ELISA-NB600-637-img0009.jpg)
![Immunohistochemistry of Rabbit Anti-PPAR delta (N terminal specific). Antigen Retrieval: HIER pH 6.2. [Right] -Neg Ctrl: Normal rabbit IgG on heart striated muscle, pH6.2 (40X). [Left Top - low mag, Left Bottom - 20X] -Staining: Diffuse cytoplasmatic positivity for PPAR delta in human myocardiocitys at 1:500.](http://images.novusbio.com/fullsize/nb600-637_rabbit-polyclonal-ppar-delta-nr1c2-antibody-235202318143153.jpg)
![Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NB100-56363-img0003.jpg "Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0006.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody [NB100-56363] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of TAK1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunohistochemistry-Paraffin-NB100-56363-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0005.jpg)
![Western Blot: ROCK1 Antibody [NB100-624] - Detection of human and mouse ROCK1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ROCK1 antibody NB100-624 (lot NB100-624-2) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ROCK1 was also immunoprecipitated by a previous lot (lot NB100-624-1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).](http://images.novusbio.com/fullsize/ROCK1-Antibody-NB100-624-img0003.jpg "Western Blot: ROCK1 Antibody [NB100-624] - Detection of human and mouse ROCK1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ROCK1 antibody NB100-624 (lot NB100-624-2) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ROCK1 was also immunoprecipitated by a previous lot (lot NB100-624-1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).")
![Western Blot: TCF7L2 Antibody (3A4) [H00006934-M06] - Analysis of TCF7L2 expression in K-562 (Cat # L009V1).](http://images.novusbio.com/fullsize/TCF7L2-Antibody-3A4-Western-Blot-H00006934-M06-img0007.jpg "Western Blot: TCF7L2 Antibody (3A4) [H00006934-M06] - Analysis of TCF7L2 expression in K-562 (Cat # L009V1).")
![Immunocytochemistry/Immunofluorescence: TCF7L2 Antibody (3A4) [H00006934-M06] - Analysis of monoclonal antibody to TCF7L2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/TCF7L2-Antibody-3A4-Immunocytochemistry-Immunofluorescence-H00006934-M06-img0005.jpg)
![Immunohistochemistry-Paraffin: TCF7L2 Antibody (3A4) [H00006934-M06] - Analysis of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/TCF7L2-Antibody-3A4-Immunohistochemistry-Paraffin-H00006934-M06-img0006.jpg)
![ELISA: TCF7L2 Antibody (3A4) [H00006934-M06] - Detection limit for recombinant GST tagged TCF7L2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TCF7L2-Antibody-3A4-ELISA-H00006934-M06-img0004.jpg)
![Western Blot: beta-Catenin Antibody (12H7) [NBP2-03615] - Analysis of extracts (35ug) from 9 different cell lines by using anti-beta Catenin monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Western-Blot-NBP2-03615-img0024.jpg "Western Blot: beta-Catenin Antibody (12H7) [NBP2-03615] - Analysis of extracts (35ug) from 9 different cell lines by using anti-beta Catenin monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY beta Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunocytochemistry-Immunofluorescence-NBP2-03615-img0015.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0022.jpg)
![Flow Cytometry: beta-Catenin Antibody (12H7) [NBP2-03615] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-beta Catenin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Flow-Cytometry-NBP2-03615-img0014.jpg)
![Western Blot: beta-Catenin Antibody (12H7) [NBP2-03615] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta Catenin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-beta Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Western-Blot-NBP2-03615-img0023.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0016.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0017.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0018.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0019.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0020.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0021.jpg)
![Flow Cytometry: beta-Catenin Antibody (12H7) [NBP2-03615] - Analysis of Hela cells, using anti-beta Catenin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Flow-Cytometry-NBP2-03615-img0013.jpg)
![Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of Mouse brain, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Western-Blot-NBP3-15842-img0008.jpg "Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of Mouse brain, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of HeLa cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Western-Blot-NBP3-15842-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts from normal (control) and beta-Catenin knockout (KO) 293T cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Knockout-Validated-NBP3-15842-img0001.jpg)
![Immunoprecipitation: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug beta-Catenin antibody (NBP3-15842). Western blot was performed from the immunoprecipitate using beta-Catenin (NBP3-15842) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Immunoprecipitation-NBP3-15842-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517284937.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517184515.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517162378.jpg)
![Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Western blot analysis of lysates from NIH/3T3 cells, using [KO Validated] beta-Catenin Rabbit mAb at 1:1000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517223995.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Western blot analysis of lysates from wild type(WT) and beta-Catenin knockout (KO) 293T(KO) cells, using [KO Validated] beta-Catenin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517202968.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-522025171893.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517223986.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human solitary fibrous tumor tissue using [KO Validated] beta-Catenin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517172874.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Human prostate cancer tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-5220251718979.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517273553.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517284993.jpg)
![Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of CAMK2D expression in transfected 293T cell line by CAMK2D monoclonal antibody (M02), clone 1A8.Lane 1: CAMK2D transfected lysate(54.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CaM-Kinase-II-delta-Antibody-1A8-Western-Blot-H00000817-M02-img0012.jpg "Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of CAMK2D expression in transfected 293T cell line by CAMK2D monoclonal antibody (M02), clone 1A8.Lane 1: CAMK2D transfected lysate(54.1 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of monoclonal antibody to CAMK2D on NIH/3T3 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CaM-Kinase-II-delta-Antibody-1A8-Immunocytochemistry-Immunofluorescence-H00000817-M02-img0010.jpg)
![Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - CAMK2D monoclonal antibody (M02), clone 1A8 Western Blot analysis of CAMK2D expression in NIH/3T3 ( Cat # L018V1 ).](http://images.novusbio.com/fullsize/CaM-Kinase-II-delta-Antibody-1A8-Western-Blot-H00000817-M02-img0007.jpg)
![Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of CAMK2D over-expressed 293 cell line, cotransfected with CAMK2D Validated Chimera RNAi ( Cat # H00000817-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK2D monoclonal antibody (M02) clone 1A8 (Cat # H00000817-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CaM-Kinase-II-delta-Antibody-1A8-Western-Blot-H00000817-M02-img0011.jpg)