| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-91662SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-99123SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96869-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89989-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-617SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33138 | |||
| IHC-Prediluted | NBP2-44584 | |||
![Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Western-Blot-H00022943-M11-img0012.jpg "Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).")
![Immunohistochemistry-Paraffin: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of monoclonal antibody to DKK1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunohistochemistry-Paraffin-H00022943-M11-img0009.jpg)
![Immunoprecipitation: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 transfected lysate using anti-DKK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DKK1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunoprecipitation-H00022943-M11-img0010.jpg)
![ELISA: Dkk-1 Antibody (2A5) [H00022943-M11] - Detection limit for recombinant GST tagged DKK1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-ELISA-H00022943-M11-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUVBL1 protein stained by RUVBL1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20245-img0012.jpg "Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUVBL1 protein stained by RUVBL1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Mouse testis. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0010.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Human colon carcinoma, using RUVBL1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0002.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Rat heart. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0007.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Mouse colon. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0008.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Rat testis. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0009.jpg)
![Immunoprecipitation: RUVBL1 Antibody [NBP2-20245] - Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 ug of RUVBL1 antibody. Western blot analysis was performed using RUVBL1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunoprecipitation-NBP2-20245-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - Endogenous RUVBL1 & RUVBL2 colocalize with HA-NP. HeLa cells were transfected with vector control, HA-NP, or HA-VP35. Twenty-four h later, the cells were fixed & processed for immunofluorescence detection of endogenous RUVBL1 or RUVBL2 in the presence of vector control, HA-NP, or HA-VP35. Representative images of (A) endogenous RUVBL1 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) & (B) endogenous RUVBL2 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) are shown. HA-NP or HA-VP35 (green), RUVBL1/2 (red), & Hoechst 33342 nuclear stain (blue) were visualized by confocal microscopy. Scale bars = 20 µM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31018511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-20245_rabbit-polyclonal-ruvbl1-antibody-31020241534318.jpg)
![Western Blot: RUVBL1 Antibody [NBP2-20245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (NBP2-20245) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20245_rabbit-polyclonal-ruvbl1-antibody-91020242063352.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0010.jpg "Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds")
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0006.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human endometrial tissue with Rabbit anti-c-jun [p Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0012.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of Phospho-c-Jun(S63) on different lysates using anti-Phospho-c-Jun(S63) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0005.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Western blot analysis of c-jun on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0007.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Human U251 glioma cell line, whole cell lysates. Cells have been treated with JNK and p38 inhibitors. WB image submited by a verified customer review.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0008.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun (p Ser63) on different lysates with Rabbit anti-c-Jun (p Ser63) antibody at 1/500 dilution. Lane 1: U87MG cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0002.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0003.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-c-jun [Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0011.jpg)
![Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate.](http://images.novusbio.com/fullsize/APC-Antibody-Western-Blot-NB100-91662-img0004.jpg "Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate.")
![Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/APC-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91662-img0006.jpg)
![Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.](http://images.novusbio.com/fullsize/APC-Antibody-Immunohistochemistry-Paraffin-NB100-91662-img0005.jpg)
![Flow Cytometry: APC Antibody [NB100-91662] - An intracellular stain was performed on HeLa cells with APC Antibody NB100-91662AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/APC-Antibody-Flow-Cytometry-NB100-91662-img0007.jpg)
![Western Blot: APC Antibody [NB100-91662] - Extracts from COLO205 cells.](http://images.novusbio.com/fullsize/APC-Antibody-Western-Blot-NB100-91662-img0001.jpg)
![Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - Paraffin-embedded human colon carcinoma tissue.](http://images.novusbio.com/fullsize/APC-Antibody-Immunohistochemistry-Paraffin-NB100-91662-img0003.jpg)
![Immunohistochemistry: MMP-7 Antibody [NBP1-99123] - MMP7 antibody was tested in human breast cancer using DAB with hematoxylin counterstain. The image on the right had the antibody blocked by preincubation with immunizing peptide.](http://images.novusbio.com/fullsize/MMP-7-Antibody-Immunohistochemistry-NBP1-99123-img0002.jpg "Immunohistochemistry: MMP-7 Antibody [NBP1-99123] - MMP7 antibody was tested in human breast cancer using DAB with hematoxylin counterstain. The image on the right had the antibody blocked by preincubation with immunizing peptide.")
![Western Blot: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII alpha/beta protein using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Primary Antibody: Mouse Anti-CaMKII alpha/beta Monoclonal Antibody (NB110-96869) at 1:1000. Analysis of CaMKII alpha/beta and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.](http://images.novusbio.com/fullsize/CaMKII-alpha-beta-p-Thr286-p-Thr287-Antibody-22B1-Western-Blot-NB110-96869-img0013.jpg "Western Blot: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII alpha/beta protein using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Primary Antibody: Mouse Anti-CaMKII alpha/beta Monoclonal Antibody (NB110-96869) at 1:1000. Analysis of CaMKII alpha/beta and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.")
![Immunocytochemistry/Immunofluorescence: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII alpha/beta Monoclonal Antibody (NB110-96869) at 1:1000 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.](http://images.novusbio.com/fullsize/CaMKII-alpha-beta-p-Thr286-p-Thr287-Antibody-22B1-Immunocytochemistry-Immunofluorescence-NB110-96869-img0016.jpg)
![Immunohistochemistry: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunohistochemistry analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII alpha/beta Monoclonal Antibody (NB110-96869) at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/CaMKII-alpha-beta-p-Thr286-p-Thr287-Antibody-22B1-Immunohistochemistry-NB110-96869-img0015.jpg)
![Immunohistochemistry: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunohistochemistry analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII alpha/beta Monoclonal Antibody (NB110-96869) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle, hair follicle, epidermis. Backskin obtained from transgenic mice.](http://images.novusbio.com/fullsize/CaMKII-alpha-beta-p-Thr286-p-Thr287-Antibody-22B1-Immunohistochemistry-NB110-96869-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-89989] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89989-img0011.jpg "Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-89989] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining in human cervix, uterine and skeletal muscle tissues . Corresponding CTNNB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89989-img0025.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human cervix, uterine shows moderate membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89989-img0020.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human skeletal muscle shows weak positivity in myocytes.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89989-img0024.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human prostate shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89989-img0023.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human duodenum shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89989-img0021.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human pancreas shows moderate membranous positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89989-img0022.jpg)
![Western Blot: TBLR1 Antibody [NB600-270] - Detection of Human and Mouse TBLR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TBLR1 antibody NB600-270 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/TBLR1-Antibody-Western-Blot-NB600-270-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: TBLR1 Antibody [NB600-270] - Detection of Human and Mouse TBLR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TBLR1 antibody NB600-270 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TBLR1 Antibody [NB600-270] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- TBLR1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TBLR1-Antibody-Immunohistochemistry-Paraffin-NB600-270-img0010.jpg)
![Immunoprecipitation: TBLR1 Antibody [NB600-270] - Detection of human TBLR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBLR1 antibody NB600-270 (lot NB600-270-2) used for IP at 6 ug per reaction. TBLR1 was also immunoprecipitated by a previous lot of this antibody (lot NB600-270-1). For blotting immunoprecipitated TBLR1, NB600-270 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/TBLR1-Antibody-Immunoprecipitation-NB600-270-img0011.jpg)
![Western Blot: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of 25 ug of various whole cell lysates or 0.5 ug of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Western-Blot-NBP2-22528-img0001.jpg "Western Blot: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of 25 ug of various whole cell lysates or 0.5 ug of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of Rho A/B/C (green) showing staining in the in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0005.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis showing staining in the cytoplasm of human hepatocarcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of RhoA/B/C (green) in NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a RhoA/B/C monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of Rho A/B/C (green) showing staining in the in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0004.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of showing staining in the cytoplasm of mouse breast tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0006.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis showing staining in the cytoplasm of human prostate carcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0007.jpg)
![Immunoprecipitation: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of RhoA/B/C was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 750 ug of HeLa cell lysate with 2 ug of a RhoA/B/C monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50 ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25 ug of HeLa cell lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rho A/B/C monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunoprecipitation-NBP2-22528-img0002.jpg)
![Western Blot: KAT3B/p300 Antibody (RW109) [NB100-617] - p300 detected in a HeLa nuclear extract using NB100-617 (1:1,000). ECL: 20 minute exposure.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW109-Western-Blot-NB100-617-img0003.jpg "Western Blot: KAT3B/p300 Antibody (RW109) [NB100-617] - p300 detected in a HeLa nuclear extract using NB100-617 (1:1,000). ECL: 20 minute exposure.")
![Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Western-Blot-NB300-749-img0007.jpg "Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).")
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 11) [NB300-749] - Mouse fibroblasts.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunocytochemistry-Immunofluorescence-NB300-749-img0004.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0006.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0005.jpg)
![Western Blot: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - analysis of Cyclin D1 in 1) C2C12, 2) HepG2, and 3) NIH3T3 lysate probed with Cyclin D1 antibody at 5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Western-Blot-NBP2-15189-img0003.jpg "Western Blot: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - analysis of Cyclin D1 in 1) C2C12, 2) HepG2, and 3) NIH3T3 lysate probed with Cyclin D1 antibody at 5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Formalin-fixed, paraffin-embedded human Mantle Cell Lymphoma stained with Cyclin D1 Antibody (DCS-6).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Immunohistochemistry-Paraffin-NBP2-15189-img0010.jpg)
![Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Flow Cytometric analysis of human Cyclin D1 on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (DCS-6).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Flow-Cytometry-NBP2-15189-img0011.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Formalin-paraffin human mantle cell lymphoma stained with Cyclin D1 Ab (Clone DCS-6). Note nuclear staining of tumor cells.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Immunohistochemistry-Paraffin-NBP2-15189-img0006.jpg)
![Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Analysis using Alexa Fluor (R) 647 conjugate of NBP2-15189. Cyclin D1 staining of HCT116 cells using Alexa Fluor 647 conjugated Cyclin D1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Flow-Cytometry-NBP2-15189-img0008.jpg)
![Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Flow Cytometry of human Cyclin D1 on Jurkat cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Antibody (DCS-6).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Flow-Cytometry-NBP2-15189-img0009.jpg)