| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-169SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-296SS | |||
| Azide and BSA Free | NBP2-80763 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22117SS | |||
| Azide and BSA Free | NBP2-81014 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-109SS | |||
| Azide and BSA Free | NBP2-80882 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85067-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29623SS | |||
| Azide and BSA Free | NBP2-80957 | |||
![Western Blot: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Analysis of endogenous p19Arf using NB200-169. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (Lanes 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (Lanes 2), were resolved by SDS-PAGE. The membrane was probed with 1ug/ml purified p19Arf (NB200-169).](http://images.novusbio.com/fullsize/p19ARF-Antibody-12-A1-1-Western-Blot-NB200-169-img0004.jpg "Western Blot: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Analysis of endogenous p19Arf using NB200-169. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (Lanes 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (Lanes 2), were resolved by SDS-PAGE. The membrane was probed with 1ug/ml purified p19Arf (NB200-169).")
![Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Immunofluorescent detection of p19Arf in wild type mouse embryo fibroblasts (MEF) at passage 5 (lanes 1, 2) and NIH3T3 cells, which have deleted the Arf gene (lanes 3, 4) were fixed in methanol/acetone and probed with NB200-169, followed by a fluorescently labeled anti-rat IgG secondary antibody (lanes 1, 3). Cells were also stained with Hoechst dye to reveal the position of nuclei (lanes 2, 4).](http://images.novusbio.com/fullsize/p19ARF-Antibody-12-A1-1-Immunocytochemistry-Immunofluorescence-NB200-169-img0003.jpg)
![Immunoprecipitation: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (Lanes 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (Lanes 3), or NB200-169 (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analysed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) from Arf null NIH3T3 cells (Lanes 1) and HA- p19Arf expressing cells (Lanes 2) were also analyzed by Western blotting, as input controls.](http://images.novusbio.com/fullsize/p19ARF-Antibody-12-A1-1-Immunoprecipitation-NB200-169-img0005.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Immunohistochemistry-Paraffin-NB100-296-img0001.jpg "Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.")
![Western Blot: HIF-1 alpha Antibody (HA111) [NB100-296] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Western-Blot-NB100-296-img0004.jpg)
![Western Blot: YAP1 Antibody (1A12) [NBP2-22117] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human YAP1 Monoclonal Antibody (Catalog # NBP2-22117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Knockout-Validated-NBP2-22117-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: YAP1 Antibody (1A12) [NBP2-22117] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human YAP1 Monoclonal Antibody (Catalog # NBP2-22117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: YAP1 Antibody (1A12) - BSA Free [NBP2-22117] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with YAP1 Antibody [1A12] (NBP2-22117) at 1 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-22117-img0012.jpg)
![Immunohistochemistry-Paraffin: YAP1 Antibody (1A12) [NBP2-22117] - Analysis of paraffin-embedded prostate cancer tissues using YAP1 mouse mAb with DAB staining. 1:200 - 1:1000](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Immunohistochemistry-Paraffin-NBP2-22117-img0009.jpg)
![Western Blot: YAP1 Antibody (1A12) [NBP2-22117] - Analysis using YAP1 mouse mAb against Hela (1), C6 (2) and Cos7 (3) cell lysate. Dilution 1:500 - 1:2000.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Western-Blot-NBP2-22117-img0008.jpg)
![Immunohistochemistry-Paraffin: YAP1 Antibody (1A12) [NBP2-22117] - Analysis of paraffin-embedded rectum cancer tissues using YAP1 mouse mAb with DAB staining. 1:200 - 1:1000](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Immunohistochemistry-Paraffin-NBP2-22117-img0006.jpg)
![Flow Cytometry: YAP1 Antibody (1A12) [NBP2-22117] - Analysis of Hela cells using YAP1 mouse mAb (green) and negative control (red). Dilution 1:200 - 1:400.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Flow-Cytometry-NBP2-22117-img0004.jpg)
![ELISA: YAP1 Antibody (1A12) [NBP2-22117] - Red: Control Antigen (100 ng); Purple: Antigen (10 ng); Green: Antigen (50 ng); Blue: Antigen (100 ng). Dilution 1:10000.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-ELISA-NBP2-22117-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Simple Western: YAP1 Antibody (1A12) [NBP2-22117] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A specific band was detected for YAP1 at approximately 81 kDa (as indicated) using 20 ug/mL of mouse Anti-YAP1 Monoclonal Antibody (Catalog # NBP2-22117). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Knockout-Validated-NBP2-22117-img0011.jpg)
![Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Taz protein expression is higher in the soleus compared with plantaris and is upregulated in response to both overload (OVL) and unload (UNL) stimuli. (a) Taz protein expression (~27 kDa) in the sham plantaris and soleus muscles. (b) Taz protein in the OVL plantaris compared with sham. (c) Taz protein in the UNL soleus compared with sham. *p <= 0.05 using a Student's t-test (a, two-tailed) or paired t-test (b and c, two-tailed), n = 6 per group. Please refer to Fig. S4 for full length Western blots. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28515468) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tafazzin-TAZ-Antibody-1B10-Western-Blot-H00006901-M12-img0010.jpg "Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Taz protein expression is higher in the soleus compared with plantaris and is upregulated in response to both overload (OVL) and unload (UNL) stimuli. (a) Taz protein expression (~27 kDa) in the sham plantaris and soleus muscles. (b) Taz protein in the OVL plantaris compared with sham. (c) Taz protein in the UNL soleus compared with sham. *p <= 0.05 using a Student's t-test (a, two-tailed) or paired t-test (b and c, two-tailed), n = 6 per group. Please refer to Fig. S4 for full length Western blots. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28515468) licensed under a CC-BY license.")
![Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Analysis of TAZ expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Tafazzin-TAZ-Antibody-1B10-Western-Blot-H00006901-M12-img0006.jpg)
![Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Analysis of TAZ expression in SW-13 (Cat # L005V1).](http://images.novusbio.com/fullsize/Tafazzin-TAZ-Antibody-1B10-Western-Blot-H00006901-M12-img0007.jpg)
![Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Analysis of TAZ expression in human uterus myoma.](http://images.novusbio.com/fullsize/Tafazzin-TAZ-Antibody-1B10-Western-Blot-H00006901-M12-img0009.jpg)
![Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Analysis of TAZ expression in human kidney.](http://images.novusbio.com/fullsize/Tafazzin-TAZ-Antibody-1B10-Western-Blot-H00006901-M12-img0008.jpg)
![ELISA: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Detection limit for recombinant GST tagged TAZ is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Tafazzin-TAZ-Antibody-1B10-ELISA-H00006901-M12-img0005.jpg)
![Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Taz protein expression is higher in the soleus compared with plantaris & is upregulated in response to both overload (OVL) & unload (UNL) stimuli. (a) Taz protein expression (~27 kDa) in the sham plantaris & soleus muscles. (b) Taz protein in the OVL plantaris compared with sham. (c) Taz protein in the UNL soleus compared with sham. *p ≤ 0.05 using a Student’s t-test (a, two-tailed) or paired t-test (b & c, two-tailed), n = 6 per group. Please refer to Fig. S4 for full length Western blots. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28515468), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006901-m12_mouse-monoclonal-tafazzin-taz-antibody-1b10-310202415392549.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0026.jpg "Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human ovarian carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0023.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug K562 whole cell lysate/extract B. 30 ug THP-1 whole cell lysate/extract 10% SDS-PAGE Bmi1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0019.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug C8D30 whole cell lysate/extract. B. 30 ug BCL-1 whole cell lysate/extract. C. 30 ug Raw264.7 whole cell lysate/extract. 10 % SDS-PAGE. Dilution: 1:1000.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0020.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug HL-60 whole cell lysate/extract.10 % SDS-PAGE. Dilution: 1:1000](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0021.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human breast carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0022.jpg)
![Immunoprecipitation: BMI-1 Antibody [NBP1-33748] - Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of BMI-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of BMI-1 protein by 2.5 ug of BMI-1antibody 10% SDS-PAGE The immunoprecipitated BMI-1protein was detected by BMI-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunoprecipitation-NBP1-33748-img0018.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-9102024209145.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog lung.BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420115062.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202419593519.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420131285.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242021927.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420134048.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242063358.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BMI-1 Antibody [NBP1-33748] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-1610202419414590.jpg)
![BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: BMI1 protein stained by BMI1 antibody (NBP1-33748) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-immunocytochemistry-immunofluorescence-261120251844101.jpg)
![Western Blot: ASH2L Antibody [NB600-250] - Detection of human ASH2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH2 antibody NB600-250 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NB600-250-img0015.jpg "Western Blot: ASH2L Antibody [NB600-250] - Detection of human ASH2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH2 antibody NB600-250 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-250] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ASH2used at a dilution of 1:1,000 ( 1ug/ml).Detection: DAB](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunohistochemistry-NB600-250-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ASH2L Antibody [NB600-250] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASH2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NB600-250-img0012.jpg)
![Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in IMR32 whole cell lysate.](http://images.novusbio.com/fullsize/n-Myc-Antibody-NMYC-1-Western-Blot-NB200-109-img0002.jpg "Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in IMR32 whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: n-Myc Antibody (NMYC-1) [NB200-109] - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/n-Myc-Antibody-NMYC-1-Immunocytochemistry-Immunofluorescence-NB200-109-img0003.jpg)
![Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in human heart tissue.](http://images.novusbio.com/fullsize/n-Myc-Antibody-NMYC-1-Western-Blot-NB200-109-img0001.jpg)
![Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining in human placenta and skeletal muscle tissues . Corresponding WWTR1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85067-img0029.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining in human placenta and skeletal muscle tissues . Corresponding WWTR1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: TAZ/WWTR1 Antibody [NBP1-85067] - Analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Western-Blot-NBP1-85067-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: TAZ/WWTR1 Antibody [NBP1-85067] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85067-img0030.jpg)
![Western Blot: TAZ/WWTR1 Antibody [NBP1-85067] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Western-Blot-NBP1-85067-img0015.jpg)
![Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining of human colon shows moderate to strong nuclear positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85067-img0024.jpg)
![Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining of human kidney shows moderate nuclear positivity in glomerular cells and a subset of cells in distal tubules.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85067-img0025.jpg)
![Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining of human placenta shows moderate to strong nuclear positivity in endothelial cells.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85067-img0026.jpg)
![Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining of human renal cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85067-img0027.jpg)
![Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining of human skeletal muscle shows weak positivity in myocytes.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85067-img0028.jpg)
![Simple Western: TAZ/WWTR1 Antibody [NBP1-85067] - Simple Western lane view shows a specific band for WWTR1 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Simple-Western-NBP1-85067-img0007.jpg)
![Simple Western: TAZ/WWTR1 Antibody [NBP1-85067] - Electropherogram image(s) of corresponding Simple Western lane view. TAZ/WWTR1 antibody was used at 1:30 dilution on RT-4 lysates(s).](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Simple-Western-NBP1-85067-img0009.jpg)
![Western Blot: SOX2 Antibody (4G8) [NBP2-29623] - Western blot detection of SOX2 in A. HEK293 cell lysate B. NCCIT cell lysate and C. Full length recombinant protein.](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Western-Blot-NBP2-29623-img0001.jpg "Western Blot: SOX2 Antibody (4G8) [NBP2-29623] - Western blot detection of SOX2 in A. HEK293 cell lysate B. NCCIT cell lysate and C. Full length recombinant protein.")
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human lung cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate immunopositivity of SOX2 protein in the cytoplasm and the staining was mainly localized in the perinuclear region of the cells [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0013.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC-P analysis of SOX2 protein in a section of human kidney using 5 ug/ml concentration of SOX2 antibody (clone 4G8). The representative image shows distinct nuclear and cytoplasmic staining pattern in cells of different types of renal tubules and blood vessels in the kidney section [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0002.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC-P analysis of SOX2 protein in a section of human renal cell carcinoma using 5 ug/ml concentration of SOX2 antibody (clone 4G8). Granular nuclear and cytoplasmic staining pattern was observed in the carcinoma cells, whereas, a weak to negligible staining was observed in the stroma areas [Magnification 40X]..](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The liver section depicted a diffused cytoplasmic staining in the hepatocytes [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0004.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human brain using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The representative photomicrograph depicts a cytoplasmic and nuclear immunopositivity of SOX2 protein in brain [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0006.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded section of normal human colon tissue using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. A weak and diffused cytoplasmic staining was observed in majority of the cells in mucosal layer of colon [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0007.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human bladder cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity for SOX2 protein [Magnification 10X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0008.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity, whereas the stroma was largely negative for SOX2 protein [Magnification 10X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0009.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human uterine cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate staining in the cytoplasm and nuclei, whereas the stroma was very weakly positive for SOX2 protein [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0010.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - Analysis of HNF1a transcriptional activity in hepatocytes following transforming growth factor B (TGFB) treatment. (B) Western blot analysis for HNF1a in protein extracts from hepatocytes in a time-course experiment following TGFB treatment. a-Glyceraldehyde 3-phosphate dehydrogenase was used as loading control. Magnification 20x. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fphar.2019.00942/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HNF1-Antibody-Western-Blot-NBP1-33596-img0023.jpg "<b>Biological Strategies Validation. </b>Western Blot: HNF1 Antibody [NBP1-33596] - Analysis of HNF1a transcriptional activity in hepatocytes following transforming growth factor B (TGFB) treatment. (B) Western blot analysis for HNF1a in protein extracts from hepatocytes in a time-course experiment following TGFB treatment. a-Glyceraldehyde 3-phosphate dehydrogenase was used as loading control. Magnification 20x. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fphar.2019.00942/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HNF1 Antibody [NBP1-33596] - TGFB overrides HNF1a constitutive expression. Immunofluorescence analysis of hepatocytes transfected with pLCPX-HNF1aMyc (HNF1) or the empty vector (CTR), treated with TGFB for 24 hr or left untreated (NT). . Cells were stained with anti-E-cadherin (green) or anti-HNF1a antibody (red). Magnification 20x. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fphar.2019.00942/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33596-img0022.jpg)
![Immunoprecipitation: HNF1 Antibody [NBP1-33596] - Immunoprecipitation of HNF1 alpha protein from HepG2 whole cell extracts using 5 ug of HNF1 alpha antibody [N1N3]. Western blot analysis was performed using HNF1 alpha antibody [N1N3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunoprecipitation-NBP1-33596-img0016.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - (A) Analysis of CBP/p300 DNA binding activity after TGF beta treatment. qPCR analysis of chromatin immunoprecipitated with anti-CBP/p300 antibody from hepatocytes untreated or treated with TGF beta (for 5 h) was performed. HNF1 alpha consensus regions embedded in the indicated target gene promoters were analyzed. A HNF1 alpha nonbound region of Neurogenin 1 promoter was utilized as negative control. Data are normalized to total chromatin input & background (control immunoprecipitation with IgG) & expressed as % input. Mean ± SEM of qPCR data obtained in triplicate from three independent experiments are reported. (B)In vivo coimmunoprecipitation of HNF1 alpha & CBP/p300 in hepatocytes untreated or treated with TGF beta (for 5 h). Cells were lysed, immunoprecipitated with anti-HNF1 alpha antibody, & then analyzed by Western blotting with the indicated antibodies. As control, the immunoprecipitation with normal goat IgG was performed. TCE, total cell extracts. Densitometric analysis of WB data from three independent experiments is shown. (C) Analysis of HNF1 alpha protein PTMs following TGF beta treatment. Nuclear extracts from control hepatocytes (upper panel) & HNF1 alpha Myc-overexpressing hepatocytes treated for 3 h with TGF beta (lower panel) or left untreated (middle panel). Samples were separated by two-dimensional gel electrophoresis followed by Western blotting with anti-Myc-Tag antibody. HNF1 alpha -specific spots are indicated by the arrow. The appearance of multiple spots in TGF beta -treated hepatocytes can be observed. **p Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HNF1 stained by HNF1 antibody [N1N3] (NBP1-33596) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-91020242041265.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 antibody [N1N3] (NBP1-33596) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-91020242091424.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-1610202419414518.jpg)
![Western Blot: SP1 Antibody [NB600-232] - Detection of Human SP1 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-SP1 antibody (NB600-232) used for WB at 0.04 ug/mL. Detection by chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SP1-Antibody-Western-Blot-NB600-232-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: SP1 Antibody [NB600-232] - Detection of Human SP1 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-SP1 antibody (NB600-232) used for WB at 0.04 ug/mL. Detection by chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: SP1 Antibody [NB600-232] - FFPE sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Affinity purified rabbit anti-Sp1 used at a dilution of 1:1,000 (1 ug/mL). Detection by DAB.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunohistochemistry-Paraffin-NB600-232-img0008.jpg)
![Western Blot: SP1 Antibody [NB600-232] - A. Whole cell lysate (50 ug for E; 10 ug for T) from HEK 293T cells that were mock transfected (E) or transfected with an Sp1 expression construct (T). B. Whole cell lysate (~700 ug) from HEK 293T cells. A. Affinity purified rabbit anti-Sp1 antibody used at the indicated concentrations. B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies BL937, using each antibody at 3.75 ug/mg lysate. Subsequently, was used at 0.04 ug/mL for WB. Detection by chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).](http://images.novusbio.com/fullsize/SP1-Antibody-Western-Blot-NB600-232-img0006.jpg)
![Western Blot: c-Myc Antibody [NB600-334] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, chicken anti-c-myc antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-334-img0005.jpg "Western Blot: c-Myc Antibody [NB600-334] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, chicken anti-c-myc antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 10 seconds.")