| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56437SS | |||
| BSA Free | NB100-56437 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29623SS | |||
| Azide and BSA Free | NBP2-80957 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-65956-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77067SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76538-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49537SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33168 | |||
| IHC-Prediluted | NBP2-44590 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89104-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13550-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56168SS | |||
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CXCR4 Antibody - Azide Free [NBP2-24862] - Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-CXCR4 (NB100-56437) at a 1:25 dilution for 1 hour at room temperature and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-24862-img0002.jpg)
![Immunohistochemistry: CXCR4 Antibody - Azide Free [NBP2-24862] - CXCR4 was detected in immersion sections of human tonsil using rabbit anti-human antibody (Catalog # NB100-56437) at 1:300 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue). Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunohistochemistry-NBP2-24862-img0003.jpg)
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Analysis of 15 ug of whole cell lysate from HeLa cells with anti-CXCR4 at 1 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0001.jpg)
![Immunohistochemistry: Vimentin Antibody (RV203) [NBP1-97671] - BTIC markers in our CNS-PNET model tumors.G: Vimentin 5X, insert- 5X digital (LCAS-R-12 weeks post-injection) H: Vimentin 20X, insert- 5X digital (LC26-R-12 weeks post-injection) TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Immunohistochemistry-NBP1-97671-img0008.jpg "Immunohistochemistry: Vimentin Antibody (RV203) [NBP1-97671] - BTIC markers in our CNS-PNET model tumors.G: Vimentin 5X, insert- 5X digital (LCAS-R-12 weeks post-injection) H: Vimentin 20X, insert- 5X digital (LC26-R-12 weeks post-injection) TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: Vimentin Antibody (RV203) [NBP1-97671] - Frozen section of human colon showing positive staining in connective tissue cells and no reactivity in epithelial cells](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Immunohistochemistry-Frozen-NBP1-97671-img0001.jpg)
![Flow Cytometry: Vimentin Antibody (RV203) [NBP1-97671] - An intracellular stain was performed on HeLa cells with NBP1-97671PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Flow-Cytometry-NBP1-97671-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) [NBP1-97671] - Paraffin section of human colon](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Immunohistochemistry-Paraffin-NBP1-97671-img0002.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV203) [NBP1-97671] - Figure 3. Immunohistochemistry with NBP1-97671 (RV203) on a frozen section of human kidney showing positive staining in blood vessels, glomeruli and connective tissue cells and no reactivity in epithelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Immunohistochemistry-Frozen-NBP1-97671-img0005.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) - BSA Free [NBP1-97671] - BTIC markers in our CNS-PNET model tumors.A: Ki67, 20X, insert- 5X digital (LCAS-R-12 weeks post-injection) B: Oct3/4, 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) C: Nestin 20x, insert- 5X digital (LCAS-R-12 weeks post-injection) D: Nestin 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) E: Sox2 40X, insert- 5X digital, (LC26-R-12 weeks post-injection) F: Sox2 40X, insert- 5X digital (LCAS-R-12 weeks post-injection) G: Vimentin 5X, insert- 5X digital (LCAS-R-12 weeks post-injection) H: Vimentin 20X, insert- 5X digital (LC26-R-12 weeks post-injection) TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97671_mouse-monoclonal-vimentin-antibody-rv203-31020241534370.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) - BSA Free [NBP1-97671] - BTIC markers in our CNS-PNET model tumors.A: Ki67, 20X, insert- 5X digital (LCAS-R-12 weeks post-injection) B: Oct3/4, 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) C: Nestin 20x, insert- 5X digital (LCAS-R-12 weeks post-injection) D: Nestin 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) E: Sox2 40X, insert- 5X digital, (LC26-R-12 weeks post-injection) F: Sox2 40X, insert- 5X digital (LCAS-R-12 weeks post-injection) G: Vimentin 5X, insert- 5X digital (LCAS-R-12 weeks post-injection) H: Vimentin 20X, insert- 5X digital (LC26-R-12 weeks post-injection) TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97671_mouse-monoclonal-vimentin-antibody-rv203-31020241535653.jpg)
![Immunohistochemistry: Vimentin Antibody (RV203) - BSA Free [NBP1-97671] - Analysis of RG, TCL & the TCL derived tumors(A1, A2) LCAS-R 2nd nsphrs/LCAS-RTL(138) 2nd nsphr, (A3) Tubb3 40x LCAS-RTL(138) 2nd nsphr, (A4) GFAP 40x LCAS-RTL(138) 2nd nsphr; (B1, B2) HE 40x LCAS-R/LCAS-RTL(138) - images show undifferentiated small round blue cells with scarce cytoplasm, (B3) HE 40x (LCAS-RTL(138) SVZ 15 weeks post-injection) - extensive proliferation of undifferentiated embryonal tumor cells infiltrating the adjacent brain parenchyma, (B4) Ki67 40x (LCAS-RTL(138) SVZ 15 weeks post-injection) - high proliferative activity of tumor cells is reflected by over 75% of cells expressing Ki-67, (C1) OCT3/4 40x (LC26-RTL(170) SVZ 12 weeks post-injection) - extensive proliferation of tumor cells invading adjacent brain parenchyma show high expression of OCT3/4 (over 80% of cells), (C2) Sox2 40x (LC26-RTL(170) SVZ 12 weeks post-injection) - extensively expressed in the tumor cells, (C3) PRAME 40x (LCAS-R 12 weeks post-injection) - extensively expressed in the tumor cells, (C4) Nestin 40x (LCAS-RTL(138) 15 weeks post-injection) - extensively expressed in the tumor cells, (D1) Vimentin 40x (LCAS-RTL(138) 15 weeks post-injection) - extensively expressed in the tumor cells within the main tumor mass & in the invading areas, (D2) BLBP 40x (LC26-RTL(170) 16 weeks post-injection) - extensively expressed in the tumor cells, (D3, D4) OTX2 20x, 40x (LC26-RTL(170) 16 weeks post-injection) - extensive expression (over 80% of cells) in tumor cells. TU-tumor; P-parenchyma; PI- perivascular invasion. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.24460), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97671_mouse-monoclonal-vimentin-antibody-rv203-310202416114726.jpg)
![Western Blot: SOX2 Antibody (4G8) [NBP2-29623] - Western blot detection of SOX2 in A. HEK293 cell lysate B. NCCIT cell lysate and C. Full length recombinant protein.](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Western-Blot-NBP2-29623-img0001.jpg "Western Blot: SOX2 Antibody (4G8) [NBP2-29623] - Western blot detection of SOX2 in A. HEK293 cell lysate B. NCCIT cell lysate and C. Full length recombinant protein.")
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human lung cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate immunopositivity of SOX2 protein in the cytoplasm and the staining was mainly localized in the perinuclear region of the cells [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0013.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC-P analysis of SOX2 protein in a section of human kidney using 5 ug/ml concentration of SOX2 antibody (clone 4G8). The representative image shows distinct nuclear and cytoplasmic staining pattern in cells of different types of renal tubules and blood vessels in the kidney section [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0002.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC-P analysis of SOX2 protein in a section of human renal cell carcinoma using 5 ug/ml concentration of SOX2 antibody (clone 4G8). Granular nuclear and cytoplasmic staining pattern was observed in the carcinoma cells, whereas, a weak to negligible staining was observed in the stroma areas [Magnification 40X]..](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The liver section depicted a diffused cytoplasmic staining in the hepatocytes [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0004.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human brain using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The representative photomicrograph depicts a cytoplasmic and nuclear immunopositivity of SOX2 protein in brain [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0006.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded section of normal human colon tissue using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. A weak and diffused cytoplasmic staining was observed in majority of the cells in mucosal layer of colon [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0007.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human bladder cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity for SOX2 protein [Magnification 10X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0008.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity, whereas the stroma was largely negative for SOX2 protein [Magnification 10X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0009.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human uterine cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate staining in the cytoplasm and nuclei, whereas the stroma was very weakly positive for SOX2 protein [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0010.jpg)
![Western Blot: PUM1 Antibody [NB100-259] - Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody used for WB at 0.1 ug/ml. Secondary: HRPconjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PUM1-Antibody-Western-Blot-NB100-259-img0009.jpg "Western Blot: PUM1 Antibody [NB100-259] - Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody used for WB at 0.1 ug/ml. Secondary: HRPconjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: PUM1 Antibody [NB100-259] - Detection of mouse Pumillio 1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody NB100-259 used for WB at 0.1 ug/ml. Secondary: HRP-conjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PUM1-Antibody-Western-Blot-NB100-259-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PUM1 Antibody [NB100-259] - Detection of human Pumillio 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293Tcells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-Pumillio 1 antibody NB100-259 (lot NB100-259-3) used for IP at 6 ug per reaction. Pumillio 1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-259-2) and another rabbit anti-Pumillio 1 antibody. For blotting immunoprecipitated Pumillio 1, NB100-259 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/PUM1-Antibody-Immunoprecipitation-NB100-259-img0008.jpg)
![Immunohistochemistry-Paraffin: NKG2D/CD314 Antibody (1D11) - Low Endotoxin [NB100-65956] - Analysis of human tonsil tissue using NKG2D/CD314 antibody. PFA fixed paraffin-embedded sections. Heat-mediated antigen retrieval was performed for 30 minutes in citrate buffer. Primary antibody dilution was 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Low-Endotoxin-Immunohistochemistry-Paraffin-NB100-65956-img0013.jpg "Immunohistochemistry-Paraffin: NKG2D/CD314 Antibody (1D11) - Low Endotoxin [NB100-65956] - Analysis of human tonsil tissue using NKG2D/CD314 antibody. PFA fixed paraffin-embedded sections. Heat-mediated antigen retrieval was performed for 30 minutes in citrate buffer. Primary antibody dilution was 1:200. Image from verified customer review.")
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Analysis using the Biotin conjugate of NB100-65956. Staining of Human peripheral blood lymphocytes with biotinylated 1D11, followed by Sav-PE.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0008.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody,](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0009.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody,](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0010.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0011.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0012.jpg)
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD314:Low Endotoxin.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0004.jpg)
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0005.jpg)
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0006.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0013.jpg "Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP")
![Immunohistochemistry: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in Human Spleen. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420324853.gif)
![<b>Genetic Strategies Validation. </b>Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITClabeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4-/- astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0010.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0001.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Recombinant Protein Test: Loading: CXCR4 partial recombinant protein (H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0007.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0008.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0009.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: Lysates/proteins at 20 ug per lane. Antibodies: NBP1-7706 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-7706 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0012.jpg)
![Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody at 2 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202419313214.gif)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420414588.jpg)
![Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Loading: 10 μg of lysate Antibodies: RHAMM/CD168 , 1 ug/mL and beta-actin, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp1-76538_rabbit-polyclonal-rhamm-cd168-antibody-western-blot-119202416527..gif "<b>Genetic Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Loading: 10 μg of lysate Antibodies: RHAMM/CD168 , 1 ug/mL and beta-actin, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody [NBP1-76538] - Analysis in rat stomach tissue lysate with antibody at 0.5 ug/mL.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Western-Blot-NBP1-76538-img0004.jpg)
![Immunohistochemistry: RHAMM/CD168 Antibody [NBP1-76538] - Human stomach tissue.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-NBP1-76538-img0003.jpg)
![Flow Cytometry: RHAMM/CD168 Antibody [NBP1-76538] - Human megakaryocytes. 47,000 CD41+ CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20 min at room temperature. Cells were then washed in 500 uL PBS/BSA/EDTA and viable DAPI cells were analysed using a Galios flow cytometer. Flow cytometry image submitted by a verified customer review. Image from the Alexa Fluor 700 version of this antibody.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Flow-Cytometry-NBP1-76538-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Immunofluorescence of RHAMM/CD168 in human stomach tissue with RHAM antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76538_rabbit-polyclonal-rhamm-cd168-antibody-2711202420243358.jpg)
![Western Blot: LIN-28A Antibody [NBP1-49537] - Hypoxia promoted an increase in the expression of SOX-2, OCT-4, KLF-4, Nanog, Lin-28A and CD133. The RT-qPCR analysis showed an upregulation in the expression of Lin-28A in a time-dependent manner in sorted A549 cells under hypoxic conditions (*P<0.05). (b) Western blot analysis showed that the transcription factors and stem cell markers were not expressed under normoxia. However, there was an increase in the expression of SOX-2 (MAB2018), OCT-4 (MAB1759), KLF-4 (AF3640), Nanog (AF1997), Lin-28A and CD133 in a time-dependent manner after hypoxia treatment in sorted A549 cells. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddiscovery2016105) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Western-Blot-NBP1-49537-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: LIN-28A Antibody [NBP1-49537] - Hypoxia promoted an increase in the expression of SOX-2, OCT-4, KLF-4, Nanog, Lin-28A and CD133. The RT-qPCR analysis showed an upregulation in the expression of Lin-28A in a time-dependent manner in sorted A549 cells under hypoxic conditions (*P<0.05). (b) Western blot analysis showed that the transcription factors and stem cell markers were not expressed under normoxia. However, there was an increase in the expression of SOX-2 (MAB2018), OCT-4 (MAB1759), KLF-4 (AF3640), Nanog (AF1997), Lin-28A and CD133 in a time-dependent manner after hypoxia treatment in sorted A549 cells. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddiscovery2016105) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LIN-28A Antibody [NBP1-49537] - (e) Immunofluorescence staining showed that A549 CD133-negative cells highly expressed Lin-28A after 48 h of 1% O2 exposure. (k) Immunofluorescence staining showed that HepG2 CD133-negative cells highly expressed Lin-28A after 48 h of 1% O2 exposure.Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddiscovery2016105) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49537-img0015.jpg)
![Immunohistochemistry: LIN-28A Antibody [NBP1-49537] - Analysis of LIN28 in E17.5 mouse embryo.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunohistochemistry-NBP1-49537-img0011.jpg)
![Western Blot: LIN-28A Antibody [NBP1-49537] - Analysis of LIN28 in NTERA-2 cell lysate.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Western-Blot-NBP1-49537-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody [NBP1-49537] - Analysis of LIN28 in NTERA-2 cells using NBP1-49537. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49537-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody [NBP1-49537] - Immunofluorescence staining showed that the newly formed GL261 spheres highly expressed Lin-28A. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddiscovery2016105) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49537-img0014.jpg)
![Western Blot: S100B Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Western-Blot-NBP2-29403-img0001.jpg "Western Blot: S100B Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9) [NBP2-29403] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunocytochemistry-Immunofluorescence-NBP2-29403-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - CODEX multiplexing image of S100b in primary melanoma of the skin. Image from verified customer review.](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0009.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100B antibody(4C4.9).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Monoclonal antibody (4C4.9) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0007.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Antibody (4C4.9)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0008.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining in human tonsil and liver tissues . Corresponding RUNX2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining in human tonsil and liver tissues . Corresponding RUNX2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89104-img0012.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human tonsil shows moderate to strong nuclear positivity in a subset lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human liver shows no positivity as expected.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0008.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human rectum shows moderate to strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0009.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human salivary gland shows moderate to strong nuclear positivity in ductal cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: RUNX2/CBFA1 Antibody [NBP1-89104] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Knockdown-Validated-NBP1-89104-img0014.jpg)
![Western Blot: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Fas/TNFRSF6/CD95 Antibody [NB120-13550] - Lane 1: Rat Brain Tissue Extract. Lane 2: Mouse Brain Tissue Extract.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-Western-Blot-NB120-13550-img0007.jpg "Western Blot: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Fas/TNFRSF6/CD95 Antibody [NB120-13550] - Lane 1: Rat Brain Tissue Extract. Lane 2: Mouse Brain Tissue Extract.")
![Immunocytochemistry/Immunofluorescence: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Representative IF of cerebellar sections at P10 using an anti-CD95/Fas antibody (green), an anti-calbindin antibody (red) to highlight Purkinje cells. For IF, Hoechst dye (blue) was used to mark nuclei. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-017-0838-1), licensed under a CC-BY license](http://images.novusbio.com/fullsize/Fas-Receptor-TNFRSF6-CD95-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13550-img0008.jpg)
![Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Analysis of human colon tumor tissue stained with Fas polyclonal antibody, at 10ug/ml.](http://images.novusbio.com/fullsize/Fas-Receptor-TNFRSF6-CD95-Antibody-Immunohistochemistry-Paraffin-NB120-13550-img0010.jpg)
![Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Analysis of human spleen tissue stained with Fas polyclonal antibody, at 10ug/ml.](http://images.novusbio.com/fullsize/Fas-Receptor-TNFRSF6-CD95-Antibody-Immunohistochemistry-Paraffin-NB120-13550-img0009.jpg)
![Western Blot: Survivin Antibody [NB100-56168] - Analysis of Normal Prostate Epithelium (NPE) and Prostate Cancer using [NB100-56168]. 100 ug/lane of each human tissue lysate was loaded per lane. Survivin was detected in 7 out of 8 prostate cancer patient specimens, but was not detected in NPE. Bands detected at predicted molecular weight of 16 kDa.](http://images.novusbio.com/fullsize/Survivin-Antibody-Western-Blot-NB100-56168-img0005.jpg "Western Blot: Survivin Antibody [NB100-56168] - Analysis of Normal Prostate Epithelium (NPE) and Prostate Cancer using [NB100-56168]. 100 ug/lane of each human tissue lysate was loaded per lane. Survivin was detected in 7 out of 8 prostate cancer patient specimens, but was not detected in NPE. Bands detected at predicted molecular weight of 16 kDa.")
![Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56168] - Analysis of Human Esophageal Cancer at 1:2000 using Survivin Antibody [NB100-56168]. A1 is a high magnification of A. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NB100-56168-img0002.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56168] - Analysis of Human Prostate Cancer array at 1:2000 using Survivin Antibody [NB100-56168]. A1 is a high magnification of A. Differential expression of Survivin is seen in tissue cores from four different prostate cancer patients. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NB100-56168-img0004.jpg)
