| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81050-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70937 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27335 | |||
| BSA Free | NB100-56599SS | |||
| Azide and BSA Free | NBP2-27335SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25142SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25156SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36428SS | |||
| Azide and BSA Free | NBP2-81008 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-297SS | |||
![Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Analysis in human placenta and cervix, uterine tissues using NBP1-81050 antibody. Corresponding GDF-15 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GDF-15-Antibody-Immunohistochemistry-Paraffin-NBP1-81050-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Analysis in human placenta and cervix, uterine tissues using NBP1-81050 antibody. Corresponding GDF-15 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GDF-15 Antibody [NBP1-81050] - Analysis in human cell line BEWO.](http://images.novusbio.com/fullsize/GDF-15-Antibody-Western-Blot-NBP1-81050-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: GDF-15 Antibody [NBP1-81050] - Staining of human cell line Hep G2 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/GDF-15-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81050-img0020.jpg)
![Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Staining of human prostate shows moderate cytoplasmic positivity in a small subset of glandular cells.](http://images.novusbio.com/fullsize/GDF-15-Antibody-Immunohistochemistry-Paraffin-NBP1-81050-img0013.jpg)
![Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/GDF-15-Antibody-Immunohistochemistry-Paraffin-NBP1-81050-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Staining of human placenta shows very strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/GDF-15-Antibody-Immunohistochemistry-Paraffin-NBP1-81050-img0016.jpg)
![Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Staining of human cervix shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/GDF-15-Antibody-Immunohistochemistry-Paraffin-NBP1-81050-img0018.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-24695] - Analysis of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate using Cyclin D1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Western-Blot-NBP2-24695-img0004.jpg "Western Blot: Cyclin D1 Antibody [NBP2-24695] - Analysis of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate using Cyclin D1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-24695] - Formalin-fixed, paraffin-embedded human small intestine stained with Cyclin D1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Immunohistochemistry-Paraffin-NBP2-24695-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-13D10-Immunocytochemistry-Immunofluorescence-NBP1-41201-img0014.jpg "Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.")
![Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Detection of RbAp48 in HeLa nuclear extract](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-13D10-Western-Blot-NBP1-41201-img0012.jpg)
![Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-13D10-Immunoprecipitation-NBP1-41201-img0017.jpg)
![Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] -SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [13D10] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-41201_mouse-monoclonal-rbbp4-rbap48-antibody-13d10-western-blot-196202494114..jpg)
![Western Blot: RNA Helicase A Antibody [NB110-40579] - The identification of host proteins immunoprecipitated with L1 ORF1p-FLAG. Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Primary antibodies used to probe western blots are indicated to the left of the images (ahnRNPL (NBP1-67852), aILF3 (EPR3627), aLARP1 (NBP1-19128), aMATR3 (NB100-1761), aNCL (NB100-1920)). Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005121), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RNA-Helicase-A-Antibody-Western-Blot-NB110-40579-img0015.jpg "Western Blot: RNA Helicase A Antibody [NB110-40579] - The identification of host proteins immunoprecipitated with L1 ORF1p-FLAG. Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Primary antibodies used to probe western blots are indicated to the left of the images (ahnRNPL (NBP1-67852), aILF3 (EPR3627), aLARP1 (NBP1-19128), aMATR3 (NB100-1761), aNCL (NB100-1920)). Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005121), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: RNA Helicase A Antibody [NB110-40579] - Immunocytochemistry of KYSE-30 cells to evaluate colocalization of RNA-helicase A (NDHII, green) and RCC2 (red). Colocalization signal is detected in nucleolus. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RNA-Helicase-A-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40579-img0014.jpg)
![Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NB110-40579] - FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-DHX9 used at a dilution of 1:200 (1 ug/mL). Detection: DAB](http://images.novusbio.com/fullsize/RNA-Helicase-A-Antibody-Immunohistochemistry-NB110-40579-img0009.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: RNA Helicase A Antibody [NB110-40579] - Detection of Human DHX9 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody NB110-40579 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RNA-Helicase-A-Antibody-Western-Blot-NB110-40579-img0012.jpg)
![Immunoprecipitation: RNA Helicase A Antibody [NB110-40579] - Detection of human DHX9 by Western blot of immunoprecipitates. Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody NB110-40579 (lot NB110-40579-2) used for IP at 6 ug per reaction. DHX9 was also immunoprecipitated by a previous lot of this antibody NB110-40579-1. For blotting immunoprecipitated DHX9, NB110-40579 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RNA-Helicase-A-Antibody-Immunoprecipitation-NB110-40579-img0013.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-464-img0034.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunohistochemistry-Paraffin-NB100-464-img0029.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-464-img0032.jpg)
![Immunoprecipitation: Chk1 Antibody [NB100-464] - Detection of human Chk1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 (lot 2) used for IP at 6 ug/mg lysate. Chk1 was also immunoprecipitated by a previous lot (lot 1) of this antibody. For blotting immunoprecipitated Chk1, NB100-464 (lot 2) was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunoprecipitation-NB100-464-img0033.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells.(G) WB for indicated proteins in clones indicated on the top. Darker exposure of ORC2 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415371972.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC1 in HCT116 p53-/- cells.(A) Strategy for insertion of a blasticidin gene & poly A site after first methionine of ORC1 in the second exon. (B) PCR on genomic DNA of indicated clones. WT: HCT116 p53-/- & ORC1+/+. ORC1 Knockout clones, B14, B48 & BP32 have an insert on both alleles of ORC1 as indicated by the absence of 0.6 kb PCR product. (C) Western blot for indicated proteins in clones indicated on the top. Darker exposure of the ORC1 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. (D) Immunoblot of soluble & chromatin-associated proteins in the clones indicated at the top. For each panel, all the lanes are from the same blot & exposure. (E) Input cell lysate & immunoprecipitates of ORC1 immunoblotted for ORC1. Darker exposure of the top blots is shown in the middle. Tubulin in the cell lysate or the IgG band in the immunoprecipitate serves as loading control.DOI://dx.doi.org/10.7554/eLife.19084.005 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415525253.jpg)
![Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1 (1:500).](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Western-Blot-NBP1-47791-img0018.jpg "Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1 (1:500).")
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - HeLa cells using anti-HES1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunocytochemistry-Immunofluorescence-NBP1-47791-img0010.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded liver using anti-Hes1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hes1.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunocytochemistry-Immunofluorescence-NBP1-47791-img0008.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded breast using anti-Hes1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0011.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded Carcinoma of liver using anti-Hes1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0012.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded endometrium using anti-Hes1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0013.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded kidney using anti-Hes1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HES-1-Antibody-OTI4H1-Immunohistochemistry-Paraffin-NBP1-47791-img0014.jpg)
![Western Blot: CD9 Antibody (5G6) [NBP2-22187] - Analysis using CD9 mAb against HEK293 (1) and CD9(AA: 37-228)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD9-Antibody-5G6-Western-Blot-NBP2-22187-img0005.jpg "Western Blot: CD9 Antibody (5G6) [NBP2-22187] - Analysis using CD9 mAb against HEK293 (1) and CD9(AA: 37-228)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD9 Antibody (5G6) [NBP2-22187] - Human endometrial stromal cells stained with anti-CD9 antibody and detected with anti-mouse IgG AlexaFluor 488 secondary antibody (Green). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD9-Antibody-5G6-Immunofluorescence-NBP2-22187-img0006.jpg)
![Immunohistochemistry-Paraffin: CD9 Antibody (5G6) [NBP2-22187] - Analysis of kidney cancer tissues using CD9 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD9-Antibody-5G6-Immunohistochemistry-Paraffin-NBP2-22187-img0003.jpg)
![Flow Cytometry: CD9 Antibody (5G6) [NBP2-22187] - Analysis of Jurkat cells using CD9 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CD9-Antibody-5G6-Flow-Cytometry-NBP2-22187-img0001.jpg)
![Immunohistochemistry-Paraffin: CD9 Antibody (5G6) [NBP2-22187] - Analysis of cervical cancer tissues using CD9 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD9-Antibody-5G6-Immunohistochemistry-Paraffin-NBP2-22187-img0002.jpg)
![ELISA: CD9 Antibody (5G6) [NBP2-22187] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/CD9-Antibody-5G6-ELISA-NBP2-22187-img0004.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Western-Blot-NB100-56599-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Immunocytochemistry-Immunofluorescence-NB100-56599-img0005.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Western-Blot-NB100-56599-img0003.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - NBP2-25142 was used to detect GST fusion proteins expressing each of the six human actin proteins. GST-fusion proteins encoding all six full length human actin gene products were run out on SDS-PAGE, transferred to nitrocellulose and reacted with NBP2-25142. The antibody stained all six proteins indicating that it is a panspecific actin antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0012.jpg "Western Blot: Actin Antibody (5J11) [NBP2-25142] - NBP2-25142 was used to detect GST fusion proteins expressing each of the six human actin proteins. GST-fusion proteins encoding all six full length human actin gene products were run out on SDS-PAGE, transferred to nitrocellulose and reacted with NBP2-25142. The antibody stained all six proteins indicating that it is a panspecific actin antibody.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (5J11) [NBP2-25142] - Beta actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NBP2-25142) at 1:2700 dilution. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Immunocytochemistry-Immunofluorescence-NBP2-25142-img0010.jpg)
![Flow Cytometry: Actin Antibody (5J11) [NBP2-25142] - analysis of HeLa cells using mouse Monoclonal Actin antibody (Orange) and Isotype control Antibody (Blue).](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Flow-Cytometry-NBP2-25142-img0008.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - Analysis of HeLa, A431, K562, MCF-7, NIH-3T3, C6, and Rat-2 cell lines.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0007.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.2 ug/mL mouse anti-Actin monoclonal (NBP2-25142, Novus Biologicals), followed by 1:2000 dilution of HRP- conjugated secondary antibody, donkey anti-mouse IgG (HAF018, R&D Systems).](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0009.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - Analysis of tissue and cell lysates probed with mouse mAb against actin, NBP2-25142, in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The same blot was simultaneously probed with chicken pAb to UCHL-1, a marker of neuronal lineage cells, in green.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (5J11) [NBP2-25142] - HeLa cells stained with NBP2-25142 (green) and also with the chicken polyclonal antibody to Vimentin (NB300-223, red). The Actin antibody stains the submembranous Actin rich cytoskeleton and also stress fibers, bundles of Actin associated with adhesion sites. The Vimentin antibody stains a quite different cytoskeletal network, the intermediate filaments. The blue stain reveals DNA in the nuclei of these cells.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Immunocytochemistry-Immunofluorescence-NBP2-25142-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156] - Mixed neuron and glia cultures stained with NBP2-25156 (green), and NB300-135 rabbit antibody to NF-H (red) and DNA (blue). NBP2-25156 reveals strong cytoplasmic staining for of dendrites and perikarya, which does not overlap with the NF-H antibody, which primarily binds to axons.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-25156-img0003.jpg "Immunocytochemistry/Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156] - Mixed neuron and glia cultures stained with NBP2-25156 (green), and NB300-135 rabbit antibody to NF-H (red) and DNA (blue). NBP2-25156 reveals strong cytoplasmic staining for of dendrites and perikarya, which does not overlap with the NF-H antibody, which primarily binds to axons.")
![Immunohistochemistry Free-Floating: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of a rat hippocampus section stained with mouse mAb to MAP2, NBP2-25156, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP2-25156 antibody labels MAP2 protein in the perikarya and dendrites of most neurons while the FOX3/NeuN antibody selectively stains nuclei and proximal soma of neuronal cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-25156-img0005.jpg)
![Western Blot: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of tissue and cell lysates using mouse mAb to MAP2, NBP2-25156, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, and [4] embryonic rat cortical neuron-glial cell lysate. A band at about 280 kDa corresponds to the MAP2A and MAP2B proteins.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Western-Blot-NBP2-25156-img0006.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of MAP2 in a 1% PFA fixed mouse brain section using anti-MAP2 (red) and NeuN (green) antibodies. Image from verified customer review.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-25156-img0004.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Staining in mouse brain. Image from a verified customer review.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-25156-img0008.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Staining of a 1% PFA fixed mouse cortical region section with anti-MAP 2 (dilution 1:300; Alexa 568 red). Image from a verified customer review.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-25156-img0007.jpg)
![Western Blot: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of VEGF R2/KDR/Flk-1 (1B6) in purified human recombinant protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Western-Blot-NBP2-36428-img0014.jpg "Western Blot: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of VEGF R2/KDR/Flk-1 (1B6) in purified human recombinant protein.")
![Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Human brain endothelial cells stained for VEGFR2 with antibody at concentration of 1:100. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-1B6-Immunocytochemistry-Immunofluorescence-NBP2-36428-img0016.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of FFPE transverse section of normal human skeletal muscle with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The myocytes showed a moderate diffused to granular staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Immunohistochemistry-Paraffin-NBP2-36428-img0013.jpg)
![Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - HepG2 cells were stained (surface) with VEGF R2/KDR/Flk-1 antibody (clone: 2B5.1B6, orange) or isotype control (mouse IgG1, blue) and positive staining observed using FITC conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Flow-Cytometry-NBP2-36428-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Detection in HUVEC cells at 1:50](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Immunocytochemistry-Immunofluorescence-NBP2-36428-img0009.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of FFPE tissue section of human normal kidney with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The cuboidal epithelial cells of of various tubules/ducts showed a specific cytoplasmic immunopositivity of protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Immunohistochemistry-Paraffin-NBP2-36428-img0010.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of FFPE tissue section of human liver cancer with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The cancer cells showed a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Immunohistochemistry-Paraffin-NBP2-36428-img0011.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of FFPE tissue section of a malignant stromal tumor of the human small bowel with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Immunohistochemistry-Paraffin-NBP2-36428-img0012.jpg)
![Western Blot: TERT Antibody (2D8) [NB100-297] - Analysis of human TERT, using NB100-297. Sample: MJ90 whole cell lysate.](http://images.novusbio.com/fullsize/TERT-Antibody-2D8-Western-Blot-NB100-297-img0010.jpg "Western Blot: TERT Antibody (2D8) [NB100-297] - Analysis of human TERT, using NB100-297. Sample: MJ90 whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: TERT Antibody (2D8) [NB100-297] - Detection of Tert (green) in HepG2 cells using NB100-297. Nuclei (Blue) are counterstained with Hoechst 33258.](http://images.novusbio.com/fullsize/TERT-Antibody-2D8-Immunocytochemistry-Immunofluorescence-NB100-297-img0009.jpg)
![Immunohistochemistry-Paraffin: TERT Antibody (2D8) [NB100-297] - TERT was detected in immersion fixed paraffin-embedded sections of human liver cancer using anti-human mouse monoclonal antibody (Catalog # NB100-297) at 1:600 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/TERT-Antibody-2D8-Immunohistochemistry-Paraffin-NB100-297-img0011.jpg)