| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36429-0.025mg | |||
| Azide and BSA Free | NBP2-81009 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0131A488-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88982-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87871-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80807-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72710 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27405 | |||
| BSA Free | NBP2-27404SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87692-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60491SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34760-0.1mg | |||
![Western Blot: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of VEGF R2/KDR/Flk-1 (clone 2C6) in partial recombinant protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Western-Blot-NBP2-36429-img0018.jpg "Western Blot: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of VEGF R2/KDR/Flk-1 (clone 2C6) in partial recombinant protein.")
![Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Antibody (1:50) was tested in HUVEC cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunocytochemistry-Immunofluorescence-NBP2-36429-img0012.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of a malignant stromal tumor of the human small bowel with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0017.jpg)
![Flow (Cell Surface): VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - HepG2 cells were stained (surface) with antibody (orange) or isotype control, mouse IgG1 (blue). Positive staining observed using FITC conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Flow-Cell-Surface-NBP2-36429-img0011.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of human normal kidney with antibody at 5 ug/ml concentration. The cuboidal epithelial cells of of various tubules/ducts showed a specific cytoplasmic immunopositivity of VEGF R2/KDR/Flk-1 protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0013.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of Human SCC of Esophagus with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm while the tumor stroma was negative for VEGF R2/KDR/Flk-1 immunopositivity.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0014.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of Human Hepatocellular Carcinoma with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0015.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Aanalysis of FFPE transverse section of normal human skeletal muscle with antibody at 5 ug/ml concentration. The myocytes showed a moderate diffused to granular staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0016.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse kidney. Citrate synthetase antibody [1761] dilution: 1:200.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Immunohistochemistry-Paraffin-NBP2-43648-img0004.jpg "Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse kidney. Citrate synthetase antibody [1761] dilution: 1:200.")
![Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract 12 % SDS-PAGE Citrate synthetase antibody [1761] dilution: 1:1000.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Western-Blot-NBP2-43648-img0005.jpg)
![Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of A. 30 ug zebrafish brain lysate/extract 10 % SDS-PAGE Citrate synthetase antibody [1761] dilution: 1:1000](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Western-Blot-NBP2-43648-img0006.jpg)
![Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE Citrate synthetase antibody [1761] dilution: 1:1000.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Western-Blot-NBP2-43648-img0007.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of H1299 xenograft. Citrate synthetase antibody dilution: 1:200.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Immunohistochemistry-Paraffin-NBP2-43648-img0001.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse duodenum. Citrate synthetase antibody [1761] dilution: 1:200.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Immunohistochemistry-Paraffin-NBP2-43648-img0002.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse heart. Citrate synthetase antibody [1761] dilution: 1:200.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Immunohistochemistry-Paraffin-NBP2-43648-img0003.jpg)
![Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (NBP2-43648) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43648_mouse-monoclonal-cs-citrate-synthase-antibody-1761-western-blot-261120251835176.jpg)
![Whole Japanese medaka extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [GT1761] (NBP2-43648) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43648_mouse-monoclonal-cs-citrate-synthase-antibody-1761-western-blot-2611202518185010.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (NBP2-43648) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43648_mouse-monoclonal-cs-citrate-synthase-antibody-1761-western-blot-261120251981024.jpg)
![Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.A. 30 ug zebrafish heart lysate/extract10% SDS-PAGECitrate synthetase antibody [GT1761] (NBP2-43648) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43648_mouse-monoclonal-cs-citrate-synthase-antibody-1761-western-blot-2611202518451320.jpg)
![HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (NBP2-43648) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43648_mouse-monoclonal-cs-citrate-synthase-antibody-1761-western-blot-261120251822204.jpg)
![Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Citrate synthetase protein stained by Citrate synthetase antibody [GT1761] (NBP2-43648) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43648_mouse-monoclonal-cs-citrate-synthase-antibody-1761-immunocytochemistry-immunofluorescence-2611202518224317.jpg)
![Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (mAb89) [DDX0131P-100] - CD40-transfected L cells stained.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-mAb89-Immunocytochemistry-Immunofluorescence-DDX0131P-100-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (mAb89) [DDX0131P-100] - CD40-transfected L cells stained.")
![Immunohistochemistry: CD40/TNFRSF5 Antibody (mAb89) [DDX0131P-100] - IHC staining on Bouin paraffinlymph node section. HumanCD40 stained with mAb 89.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-mAb89-Immunohistochemistry-DDX0131P-100-img0004.jpg)
![Western Blot: Rheb Antibody (2C11) [H00006009-M01] - RHEB monoclonal antibody (M01), clone 2C11. Analysis of RHEB expression in NIH/3T3.](http://images.novusbio.com/fullsize/Rheb-Antibody-2C11-Western-Blot-H00006009-M01-img0010.jpg "Western Blot: Rheb Antibody (2C11) [H00006009-M01] - RHEB monoclonal antibody (M01), clone 2C11. Analysis of RHEB expression in NIH/3T3.")
![Western Blot: Rheb Antibody (2C11) [H00006009-M01] - RHEB monoclonal antibody (M01), clone 2C11. Analysis of RHEB expression in PC-12.](http://images.novusbio.com/fullsize/Rheb-Antibody-2C11-Western-Blot-H00006009-M01-img0007.jpg)
![Western Blot: Rheb Antibody (2C11) [H00006009-M01] - RHEB monoclonal antibody (M01), clone 2C11. Analysis of RHEB expression in Raw 264.7.](http://images.novusbio.com/fullsize/Rheb-Antibody-2C11-Western-Blot-H00006009-M01-img0008.jpg)
![Western Blot: Rheb Antibody (2C11) [H00006009-M01] - RHEB monoclonal antibody (M01), clone 2C11. Analysis of RHEB expression in Jurkat.](http://images.novusbio.com/fullsize/Rheb-Antibody-2C11-Western-Blot-H00006009-M01-img0009.jpg)
![ELISA: Rheb Antibody (2C11) [H00006009-M01] - Detection limit for recombinant GST tagged RHEB is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Rheb-Antibody-2C11-ELISA-H00006009-M01-img0011.jpg)
![Western Blot: RHOT2 Antibody [NBP1-88982] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/RHOT2-Antibody-Western-Blot-NBP1-88982-img0009.jpg "Western Blot: RHOT2 Antibody [NBP1-88982] - Analysis in human cell line HEK 293.")
![Immunocytochemistry/Immunofluorescence: RHOT2 Antibody [NBP1-88982] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RHOT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88982-img0011.jpg)
![Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982] - Staining of human Liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/RHOT2-Antibody-Immunohistochemistry-Paraffin-NBP1-88982-img0014.jpg)
![Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982] - Staining of human Cerebral cortex shows moderate granular cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/RHOT2-Antibody-Immunohistochemistry-Paraffin-NBP1-88982-img0012.jpg)
![Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982] - Staining of human Heart muscle shows moderate granular cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/RHOT2-Antibody-Immunohistochemistry-Paraffin-NBP1-88982-img0013.jpg)
![Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982] - Staining of human Skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/RHOT2-Antibody-Immunohistochemistry-Paraffin-NBP1-88982-img0015.jpg)
![Simple Western: RHOT2 Antibody [NBP1-88982] - Simple Western lane view shows a specific band for RHOT2 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/RHOT2-Antibody-Simple-Western-NBP1-88982-img0007.jpg)
![Simple Western: RHOT2 Antibody [NBP1-88982] - Electropherogram image(s) of corresponding Simple Western lane view. RHOT2 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/RHOT2-Antibody-Simple-Western-NBP1-88982-img0008.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0013.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human cervix shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0010.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0011.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0012.jpg)
![Western Blot: Hornerin Antibody [NBP1-80807] - Hornerin expression in HCC cell lines. Western blot analysis of Hornerin expression levels in a panel of HCC cell lines. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4719-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Western-Blot-NBP1-80807-img0013.jpg "Western Blot: Hornerin Antibody [NBP1-80807] - Hornerin expression in HCC cell lines. Western blot analysis of Hornerin expression levels in a panel of HCC cell lines. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4719-5), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Hornerin Antibody [NBP1-80807] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80807-img0006.jpg)
![Immunohistochemistry: Hornerin Antibody [NBP1-80807] - Hornerin overpression in human HCC tumor tissues. Immunohistochemistry of Hornerin expression in hepatocellular carcinoma (HCC) tissues. Hornerin expression in the cytoplasm and membrane is scored as negative (a, e), weak (b, f), moderate (c, g), and strong (d, h). Original magnification, x 100 (a-d); x 400 (e-h) Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4719-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Immunohistochemistry-NBP1-80807-img0011.jpg)
![Immunohistochemistry-Paraffin: Hornerin Antibody [NBP1-80807] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Immunohistochemistry-Paraffin-NBP1-80807-img0007.jpg)
![Immunohistochemistry-Paraffin: Hornerin Antibody [NBP1-80807] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Immunohistochemistry-Paraffin-NBP1-80807-img0008.jpg)
![Immunohistochemistry-Paraffin: Hornerin Antibody [NBP1-80807] - Staining of human skin shows moderate to strong cytoplasmic granular positivity in the cornified layer.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Immunohistochemistry-Paraffin-NBP1-80807-img0009.jpg)
![Immunohistochemistry-Paraffin: Hornerin Antibody [NBP1-80807] - Staining of human testis shows weak positivity in peritubular myoid cells.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Immunohistochemistry-Paraffin-NBP1-80807-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Hornerin Antibody [NBP1-80807] - Hornerin expression in HCC cell lines. RNR shRNAs inhibited the expression of Hornerin in PLC/PRF/5 and QGY-7703 cells Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4719-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hornerin-Antibody-Knockdown-Validated-NBP1-80807-img0012.jpg)
![Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Western-Blot-NBP1-47839-img0020.jpg "Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK4.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0013.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded mouse spleen tissue using MKK4/MEK4 antibody (OTI8A8). Image from verified customer review.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0022.jpg)
![Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEK4 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Flow-Cytometry-NBP1-47839-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of HepG2 cells using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0012.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0014.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0015.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human pancreas tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0017.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0018.jpg)
![Immunoprecipitation: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunoprecipitation-NBP1-47839-img0021.jpg)
![Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Western-Blot-NBP2-27404-img0001.jpg "Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0009.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells and splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0006.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 0.1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0007.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0008.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) - BSA Free [NBP2-27404] - An intracellular stain was performed on THP-1 cells with TLR3 Antibody [27N3D4] NBP2-27405AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/nbp2-27404_rat-monoclonal-tlr3-antibody-27n3d4-177202495942.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) - BSA Free [NBP2-27404] - An intracellular stain was performed on Raw264.7 cells with TLR3 [27N3D4] Antibody NBP2-27405AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/nbp2-27404_rat-monoclonal-tlr3-antibody-27n3d4-17720241044614.jpg)
![Western Blot: Emerin Antibody [NBP1-87692] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Emerin-Antibody-Western-Blot-NBP1-87692-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Emerin Antibody [NBP1-87692] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Emerin Antibody [NBP1-87692] - Staining of human cell line U-251 MG shows localization to nuclear membrane & endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87692-img0010.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692] - Staining of human skeletal muscle shows moderate nuclear membrane positivity in myocytes.](http://images.novusbio.com/fullsize/Emerin-Antibody-Immunohistochemistry-Paraffin-NBP1-87692-img0006.jpg)
![Western Blot: Emerin Antibody [NBP1-87692] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/Emerin-Antibody-Western-Blot-NBP1-87692-img0011.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692] -Staining of human heart muscle shows strong positivity in nuclear membrane in cardiomyocytes.](http://images.novusbio.com/fullsize/antibody/nbp1-87692_rabbit-polyclonal-emerin-antibody-immunohistochemistry-paraffin-21122023105440..jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692] -Staining of human fallopian tube shows moderate positivity in nuclear membrane in glandular cells and smooth muscle cells with additional weak cytoplasmic positivity.](http://images.novusbio.com/fullsize/antibody/nbp1-87692_rabbit-polyclonal-emerin-antibody-immunohistochemistry-paraffin-21122023105539..jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692]- Staining of human testis shows moderate positivity in nuclear membrane in cells in seminiferous ducts and leydig cells with weak cytoplasmic positivity.](http://images.novusbio.com/fullsize/antibody/nbp1-87692_rabbit-polyclonal-emerin-antibody-immunohistochemistry-paraffin-21122023105658..jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot using lysates from U20S parental cell line and Dynamin knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Dynamin Monoclonal Antibody (Catalog # NB110-60491) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Dynamin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Knockout-Validated-NB110-60491-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot using lysates from U20S parental cell line and Dynamin knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Dynamin Monoclonal Antibody (Catalog # NB110-60491) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Dynamin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Simple Western: Dynamin Antibody (3G4B6) [NB110-60491] - Simple Western lane view shows a specific band for Dynamin in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Simple-Western-NB110-60491-img0012.jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using Dynamin mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Western-Blot-NB110-60491-img0003.jpg)
![Immunoprecipitation: Dynamin Antibody (3G4B6) [NB110-60491] - Antibody-bead conjugates were prepared by adding 1.0 ug of antibody to 500 ul of PBS with 0,01% triton X-100 in a microcentrifuge tube and 30ul of protein G Sepharose beads. Tubes were rocked O/N at 4 degrees C followed by several washes to remove unbound antibodies. U2OS were collected in HEPES buffer (20 mM HEPES, 100 mM sodium chloride, 1 mM EDTA, 1% Triton X-100, pH 7.4) supplemented with protease inhibitor. Lysates are rocked 30 min at 4 degrees C and spun at 110,000xg for 15 min at 4 degrees C. One ml aliquots at 1 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4 degrees C. Following centrifugation, the unbound fractions were collected, and beads were subsequently washed three times with 1 ml of HEPES lysis buffer and processed for SDS-PAGE and immunoblot. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Immunoprecipitation-NB110-60491-img0014.jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using anti-Dynamin monoclonal antibody against truncated Dynamin recombinant protein (1), SK-N-SH (2) and NIH3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Western-Blot-NB110-60491-img0002.jpg)
![Immunohistochemistry-Paraffin: Dynamin Antibody (3G4B6) [NB110-60491] - Immunohistochemical analysis of paraffin-embedded human Lymph tissue, glioma tissue and cerebellum tissue, showing membrane localization using Dynamin antibody with DAB staining.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Immunohistochemistry-Paraffin-NB110-60491-img0004.jpg)
![Flow Cytometry: Dynamin Antibody (3G4B6) [NB110-60491] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dynamin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Flow-Cytometry-NB110-60491-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Dynamin Antibody (3G4B6) [NB110-60491] - U2OS WT and DNM1 KO cells were labelled with a green or a far red fluorescence dye, respectively. WT and KO cells were mixed and plated to a 1 :1 ratio on coverslips. Cells were stained with and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (WT), red (antibody staining) and far-red (KO) channels was performed. Representative grayscale image of the red channel is shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Antibody dilution used: 1/1000. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Knockout-Validated-NB110-60491-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunocytochemistry-Immunofluorescence-NBP2-32808-img0009.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Staining of paraffin-embedded Leiomyosarcoma using the Azide and BSA-Free version of alpha-Smooth Muscle Actin (SPM332) Antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunohistochemistry-Paraffin-NBP2-32808-img0004.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0008.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP. Image from the PerCP version of this antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0006.jpg)