| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-22131SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81943-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80611-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73664 | |||
![Western Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Western-Blot-NBP2-43535-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 (acetyl Lys18) protein stained by Histone H3 (acetyl Lys18) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-43535-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of rat fore brain. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0007.jpg)
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H3K18ac (acetyl Lys18) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Chromatin-Immunoprecipitation-NBP2-43535-img0001.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse duodenum. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse prostate. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0006.jpg)
![Dot Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of anti-Histone H3 (acetyl Lys18) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys18) antibody 1:5000 dilution. A: Peptide samples of H3K18 un B: Peptide samples of H3K18ac C: Peptide samples of H3K9un D: Peptide samples of H3K9ac E: Peptide samples of H3K14un F: Peptide samples of H3K14ac G: Peptide samples of H3K27un H: Peptide samples of H3K27ac .](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Dot-Blot-NBP2-43535-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of HeLa whole cell lysate/extract: A. 30 ug whole cell lysate/extract of Histone H3 (acetyl Lys18) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B. Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of Histone H3 (acetyl Lys18) protein by 2.5 ug of Histone H3 (acetyl Lys18) antibody 12% SDS-PAGE The immunoprecipitated Histone H3 protein (acetyl Lys18) was detected by Histone H3 (acetyl Lys18) antibody. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunoprecipitation-NBP2-43535-img0008.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420122076.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242014689.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects Histone H3K18ac protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.Histone H3K18ac stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420115031.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242014664.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420115087.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at chromosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242011459.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects Histone H3K18ac protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Histone H3K18ac stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242063367.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at chromosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-9102024205654.jpg)
![Histone H3 (acetyl Lys18) antibody detects Histone H3 (acetyl Lys18) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H3 (acetyl Lys18) protein stained by Histone H3 (acetyl Lys18) antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-immunocytochemistry-immunofluorescence-261120251823621.jpg)
![Western Blot: CaMKII beta Antibody [NB120-22131] - Analysis of CAMKIIb in rat brain lysate using NB120-22131 at 1:2000.](http://images.novusbio.com/fullsize/CaMKII-beta-Antibody-Western-Blot-NB120-22131-img0002.jpg "Western Blot: CaMKII beta Antibody [NB120-22131] - Analysis of CAMKIIb in rat brain lysate using NB120-22131 at 1:2000.")
![Immunocytochemistry/Immunofluorescence: CaMKII beta Antibody [NB120-22131] - Staining of CAMKIIb using NB120-22131 in rat hippocampal neurons at a dilution of 1:100, note that staining is rather weak.](http://images.novusbio.com/fullsize/CaMKII-beta-Antibody-Immunocytochemistry-Immunofluorescence-NB120-22131-img0001.jpg)
![Western Blot: CaMKII beta Antibody [NB120-22131] - Analysis of CAMKII beta in A) human, B) mouse and C) rat brain lysate using CAMKII beta antibody at 1:2000.](http://images.novusbio.com/fullsize/CaMKII-beta-Antibody-Western-Blot-NB120-22131-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody [NB100-81943] - Staining of methanol-fixed MCF7 cells NB100-81943](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81943-img0006.jpg "Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody [NB100-81943] - Staining of methanol-fixed MCF7 cells NB100-81943")
![Immunoprecipitation: GSK-3 alpha Antibody [NB100-81943] - Human HaCaT keratinocyte lysates with GSK3 alpha Antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Immunoprecipitation-NB100-81943-img0010.jpg)
![Western Blot: GSK-3 alpha Antibody [NB100-81943] - Analysis of extracts from 293 cells using NB100-81943](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Western-Blot-NB100-81943-img0008.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha Antibody [NB100-81943] - Analysis of paraffin- embedded human breast carcinoma tissue using NB100-81943(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81943_rabbit-polyclonal-gsk-3-alpha-antibody-10320252332100.jpg)
![Western Blot: MAPKAPK2 Antibody (2B3) [H00009261-M01] - MAPKAPK2 monoclonal antibody (M01), clone 2B3 Analysis of MAPKAPK2 expression in K-562.](http://images.novusbio.com/fullsize/MAPKAPK2-Antibody-2B3-Western-Blot-H00009261-M01-img0016.jpg "Western Blot: MAPKAPK2 Antibody (2B3) [H00009261-M01] - MAPKAPK2 monoclonal antibody (M01), clone 2B3 Analysis of MAPKAPK2 expression in K-562.")
![Immunocytochemistry/Immunofluorescence: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of monoclonal antibody to MAPKAPK2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MAPKAPK2-Antibody-2B3-Immunocytochemistry-Immunofluorescence-H00009261-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of monoclonal antibody to MAPKAPK2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MAPKAPK2-Antibody-2B3-Immunohistochemistry-Paraffin-H00009261-M01-img0013.jpg)
![Proximity Ligation Assay: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of protein-protein interactions between AKT1 and MAPKAPK2. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-MAPKAPK2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MAPKAPK2-Antibody-2B3-Proximity-Ligation-Assay-H00009261-M01-img0009.jpg)
![Immunoprecipitation: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of MAPKAPK2 transfected lysate using anti-MAPKAPK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPKAPK2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/MAPKAPK2-Antibody-2B3-Immunoprecipitation-H00009261-M01-img0014.jpg)
![ELISA: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Detection limit for recombinant GST tagged MAPKAPK2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MAPKAPK2-Antibody-2B3-ELISA-H00009261-M01-img0017.jpg)
![Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Western blot analysis of FAK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Western-Blot-NBP2-67327-img0012.jpg "Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Western blot analysis of FAK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi")
![Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunocytochemistry-Immunofluorescence-NBP2-67327-img0007.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0011.jpg)
![Flow Cytometry: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of Hela cells with FAK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Flow-Cytometry-NBP2-67327-img0001.jpg)
![Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of FAK on different lysates using anti-FAK antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Western-Blot-NBP2-67327-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunocytochemistry-Immunofluorescence-NBP2-67327-img0002.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0003.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse brain tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0004.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0005.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded huamn liver cancer tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0008.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0009.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0010.jpg)
![Western Blot: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Western blot analysis of PDGFR alpha on SHG-44 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PDGFR-alpha-Antibody-JF104-6-Western-Blot-NBP2-67025-img0008.jpg "Western Blot: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Western blot analysis of PDGFR alpha on SHG-44 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Staining PDGFR alpha in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PDGFR-alpha-Antibody-JF104-6-Immunocytochemistry-Immunofluorescence-NBP2-67025-img0003.jpg)
![Immunohistochemistry-Paraffin: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Analysis of paraffin-embedded mouse uterus tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PDGFR-alpha-Antibody-JF104-6-Immunohistochemistry-Paraffin-NBP2-67025-img0006.jpg)
![Flow Cytometry: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Analysis of NIH/3T3 cells with PDGFR alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PDGFR-alpha-Antibody-JF104-6-Flow-Cytometry-NBP2-67025-img0001.jpg)
![Western Blot: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Analysis of PDGFR alpha on NIH/3T3 cells lysates using anti-PDGFR alpha antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/PDGFR-alpha-Antibody-JF104-6-Western-Blot-NBP2-67025-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Staining PDGFR alpha in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PDGFR-alpha-Antibody-JF104-6-Immunocytochemistry-Immunofluorescence-NBP2-67025-img0002.jpg)
![Immunohistochemistry-Paraffin: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Analysis of paraffin-embedded mouse brain tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PDGFR-alpha-Antibody-JF104-6-Immunohistochemistry-Paraffin-NBP2-67025-img0004.jpg)
![Immunohistochemistry-Paraffin: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Analysis of paraffin-embedded mouse colon tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PDGFR-alpha-Antibody-JF104-6-Immunohistochemistry-Paraffin-NBP2-67025-img0005.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Analysis in human cerebral cortex and pancreas tissues. Corresponding YWHAB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Analysis in human cerebral cortex and pancreas tissues. Corresponding YWHAB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: 14-3-3 beta/alpha Antibody [NBP1-80611] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-80611-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80611-img0003.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0010.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NBP1-80611] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-80611-img0004.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0007.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human skin shows moderate to strong cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0009.jpg)
![Western Blot: p38 Antibody [NBP2-19662] - Wild-type (WT) and p38 MAPK knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p38-Antibody-Western-Blot-NBP2-19662-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p38 Antibody [NBP2-19662] - Wild-type (WT) and p38 MAPK knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: p38 alpha Antibody [NBP2-19662] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-Western-Blot-NBP2-19662-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: p38 Antibody [NBP2-19662] - HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: p38 MAPK protein stained by p38 MAPK antibody [N1C3-2] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize/p38-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19662-img0006.jpg)
![Immunohistochemistry-Paraffin: p38 Antibody [NBP2-19662] - Paraffin-embedded human lung adenocarcinoma. p38 antibody [N1C3-2] diluted at 1:250.](http://images.novusbio.com/fullsize/p38-Antibody-Immunohistochemistry-Paraffin-NBP2-19662-img0003.jpg)
![Immunohistochemistry-Paraffin: p38 Antibody [NBP2-19662] - Paraffin-embedded rat brain. p38 MAPK antibody [N1C3-2] diluted at 1:500.](http://images.novusbio.com/fullsize/p38-Antibody-Immunohistochemistry-Paraffin-NBP2-19662-img0002.jpg)
![p38 Antibody [NBP2-19662] -Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-19662_rabbit-polyclonal-p38-alpha-antibody-western-blot-184202375333.jpg)
![p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: p38 MAPK protein stained by p38 MAPK antibody [N1C3-2] (NBP2-19662) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-19662_rabbit-polyclonal-p38-alpha-antibody-immunocytochemistry-immunofluorescence-261120251995418.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and p38 MAPK knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] (NBP2-19662) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19662_rabbit-polyclonal-p38-alpha-antibody-western-blot-261120251837242.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: Histone H3 antibody diluted at 1:500. Red: alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0018.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: Histone H3 antibody diluted at 1:500. Red: alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500.")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0002.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse colon. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse brain. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0004.jpg)
![Immunoprecipitation: HIST1H3A Antibody [NBP2-16842] - IP Sample: Raji whole cell lysate/extract A : Control with 3 ug of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 ug of Histone H3 antibody15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunoprecipitation-NBP2-16842-img0009.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16842] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody (NBP2-16842) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16842_rabbit-polyclonal-hist1h3a-antibody-9102024202460.jpg)
![Western Blot: ATM Antibody [NB100-678] - Samples: A) Whole cell lysate (50 ug) from human L-40 (WT) or AT-59 (AT) cells. B) Whole cell lysate (1 mg) from WT cells. Antibody: Affinity purified rabbit ATM antibody [NB100-678] used at the indicated concentrations for WB and at 0.5 ug/mg lysate for IP. Immunoprecipitated ATM was blotted with a monoclonal antibody to ATM. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~370 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0003.jpg "Western Blot: ATM Antibody [NB100-678] - Samples: A) Whole cell lysate (50 ug) from human L-40 (WT) or AT-59 (AT) cells. B) Whole cell lysate (1 mg) from WT cells. Antibody: Affinity purified rabbit ATM antibody [NB100-678] used at the indicated concentrations for WB and at 0.5 ug/mg lysate for IP. Immunoprecipitated ATM was blotted with a monoclonal antibody to ATM. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~370 kDa. Theoretical molecular weight 351 kDa.")
![Western Blot: ATM Antibody [NB100-678] - MDA-MB-231 and MCF-7 whole cell lysates were loaded with 50 ug/lane. 8% SDS-PAGE. ATM Antibody (NB100-678) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0009.jpg)
![Immunohistochemistry: ATM Antibody [NB100-678] - FFPE section of human prostate-nodular hypertrophy. Affinity purified rabbit [NB100-678] used at a dilution of 1:1000 ( 1ug/mL). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/ATM-Antibody-Immunohistochemistry-NB100-678-img0007.jpg)
![Western Blot: ATM Antibody [NB100-678] - Analysis in whole cell lysates from human lung cancer lines with [NB100-678]. Theoretical molecular weight 351 kDa. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0008.jpg)
![Western Blot: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase D2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Protein Kinase D2.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Western-Blot-NBP2-02984-img0015.jpg "Western Blot: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase D2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Protein Kinase D2.")
![Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Protein Kinase D2.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunocytochemistry-Immunofluorescence-NBP2-02984-img0010.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Human Kidney tissue using anti-Protein Kinase D2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunohistochemistry-Paraffin-NBP2-02984-img0014.jpg)
![Flow Cytometry: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Protein Kinase D2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Flow-Cytometry-NBP2-02984-img0009.jpg)
![Western Blot: Protein Kinase D2 Antibody (5G1) [NBP2-02984] Analysis of extracts (35ug) from 9 different cell lines by using anti-Protein Kinase D2 monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Western-Blot-NBP2-02984-img0008.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Protein Kinase D2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunohistochemistry-Paraffin-NBP2-02984-img0011.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Protein Kinase D2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunohistochemistry-Paraffin-NBP2-02984-img0012.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Protein Kinase D2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunohistochemistry-Paraffin-NBP2-02984-img0013.jpg)
![Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NB100-56363-img0003.jpg "Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0006.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody [NB100-56363] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of TAK1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunohistochemistry-Paraffin-NB100-56363-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0005.jpg)