| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2383SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2080SS | |||
| Azide and BSA Free | NBP2-80733 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36573SS | |||
| Azide and BSA Free | NBP2-80951 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77680SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-03499SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB300-184-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87833-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72710 | |||
![Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0009.jpg "Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).")
![Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [NB100-2383] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2383-img0007.jpg)
![Western Blot: PDK-1 Antibody [NB100-2383] - Detection of PDK-1 in human heart lysate using NB 100-2383 at 2 ug/ml.](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) [NB100-2080] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FGFR1 Antibody [M17D10] (NB100-2080) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FGFR1-Antibody-M17D10-Immunocytochemistry-Immunofluorescence-NB100-2080-img0011.jpg "Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) [NB100-2080] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FGFR1 Antibody [M17D10] (NB100-2080) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) [NB100-2080] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FGFR1 Antibody [M17D10] conjugated to Alexa Fluor 488 (NB100-2080AF488) at 10 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FGFR1-Antibody-M17D10-Immunocytochemistry-Immunofluorescence-NB100-2080-img0012.jpg)
![Western Blot: FGF R1 Antibody (M17D10) [NB100-2080] - Total protein from MOLT-4 (lane 1) and Ntera-2 (lane 2) was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/mL of anti-FGF R1. FGF R1 protein was detected at ~150 kDa using an anti-mouse HRP secondary antibody.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Western-Blot-NB100-2080-img0004.jpg)
![Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/ml concentration. Specific membrane-cytoplasmic immunopositivity of FGF R1 protein was observed in SCC cells whereas the nuclei of SCC cells as well as the tumor stroma was largely negative for this protein.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Immunohistochemistry-Paraffin-NB100-2080-img0008.jpg)
![Flow Cytometry: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/mL) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Flow-Cytometry-NB100-2080-img0010.jpg)
![Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FFPE tissue section of human normal skin using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. Cells of the epidermal layer showed a very strong membrane-cytoplasmic staining with relatively weak nuclear immunopositivity for for this protein. The stratum basale / bottom layer of keratinocytes in the epidermis showed a membrane-cytoplasmic stainin for FGF R1.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Immunohistochemistry-Paraffin-NB100-2080-img0006.jpg)
![Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FFPE tissue section of human liver using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. The hepatocytes depicted an expected and specific membrane-cytoplasmic-nuclear immunostaining of FGF R1 protein.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Immunohistochemistry-Paraffin-NB100-2080-img0007.jpg)
![Flow Cytometry: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Flow-Cytometry-NB100-2080-img0009.jpg)
![Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of SLC31A1/CTR1 in MCF7 and HeLa at 2 ug/ml.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Western-Blot-NBP2-36573-img0001.jpg "Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of SLC31A1/CTR1 in MCF7 and HeLa at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti SLC31A1/CTR1 (5E6.1B4) NBP2-36573 at a 1:50 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-36573-img0009.jpg)
![Immunohistochemistry: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - IL-17 induces cellular copper uptake through induction of STEAP4. Western blots analysis for copper trafficking-associated proteins from lysates of mouse colon epithelial organoids treated with different inflammatory cytokines for 8h. Three independent experiments were done and representative blots were shown. Untr untreated. IL-17 primed or unprimed mouse colon organoids were stained for STEAP4 (St4), E-cadherin (E-cad) and SLC31A1/CTR1 and integrin alpha. Scale bar, 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-14698-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-NBP2-36573-img0010.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - STEAP4 promotes colitis-associated colon tumorigenesis. Western blot analysis of colon tissue lysates from mice treated with DSS water for indicated days. Western blot analysis of colon tissue lysates from mice treated with DSS water for indicated days. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32060280/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Western-Blot-NBP2-36573-img0011.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a formalin fixed and paraffin embedded tissue section of human urinary bladder transitional cell carcinoma (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0002.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a FFPE tissue section of normal breast tissue from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The antibody generated a very strong cytoplasmic staining in the myo-epithelial cells in the tested section.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0008.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a FFPE tissue section of normal skin from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The antibody developed a strong cytoplasmic staining in the outer layer of stratum spinosum and the cells of stratum granulosum/ granular layer of skin epidermis. The endothelial cells in the dermis of the section also showed positivity for SLC31A1/CTR1.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0006.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Aanalysis of a FFPE tissue section of human small bowel malignant stromal tumor (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The cancer cells developed moderate to strong positivity for CTR1/SLC31A1 protein.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0007.jpg)
![Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Western-Blot-NBP1-77680-img0009.jpg "Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.")
![Immunocytochemistry/Immunofluorescence: IGF-I R Antibody [NBP1-77680] - IGF1 receptor (EC) antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77680-img0008.jpg)
![Immunohistochemistry-Paraffin: IGF-I R Antibody [NBP1-77680] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney cancer xenograft using 1:200 dilution of IFG-I R antibody (NBP1-77680). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunohistochemistry-Paraffin-NBP1-77680-img0010.jpg)
![Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGF-I R in human pancreatic cancer cell lines (MiaPaCa-2, AsPC-1, and BxPC-3) using anti-IGF-I R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Western-Blot-NBP1-77680-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: TrkB [p Tyr816] Antibody [NBP1-03499] - Immunofluorescence double staining assay of BDNF and p-TrkB in the SDH. BDNF and p-TrkB (red), NeuN, GFAP, and OX-42( green), co-localization (yellow). BDNF was mainly colocalized in neurons which mainly located in Laminate II to IV. And TrkB receptors expressed in neurons, microglia, and astrocytes. Image collected and cropped by CiteAb from the following publication (jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-020-1704-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TrkB-[p-Tyr816]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03499-img0001.jpg "Immunocytochemistry/Immunofluorescence: TrkB [p Tyr816] Antibody [NBP1-03499] - Immunofluorescence double staining assay of BDNF and p-TrkB in the SDH. BDNF and p-TrkB (red), NeuN, GFAP, and OX-42( green), co-localization (yellow). BDNF was mainly colocalized in neurons which mainly located in Laminate II to IV. And TrkB receptors expressed in neurons, microglia, and astrocytes. Image collected and cropped by CiteAb from the following publication (jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-020-1704-0), licensed under a CC-BY license.")
![Western Blot: CaMKII alpha [p Thr286] Antibody [NB300-184] - Anaylsis of Rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k beta-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/CaMKII-alpha-[p-Thr286]-Antibody-Western-Blot-NB300-184-img0003.jpg "Western Blot: CaMKII alpha [p Thr286] Antibody [NB300-184] - Anaylsis of Rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k beta-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Immunocytochemistry/Immunofluorescence: Fyn Antibody (FYN-01) [NB500-517] - Staining of Fyn in murine transformed fibroblasts using anti-Fyn (FYN-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/Fyn-Antibody-FYN-01-Immunocytochemistry-Immunofluorescence-NB500-517-img0004.jpg "Immunocytochemistry/Immunofluorescence: Fyn Antibody (FYN-01) [NB500-517] - Staining of Fyn in murine transformed fibroblasts using anti-Fyn (FYN-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).")
![Immunocytochemistry/Immunofluorescence: Fyn Antibody (FYN-01) [NB500-517] - Staining of Fyn in human primary fibroblasts using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/Fyn-Antibody-FYN-01-Immunocytochemistry-Immunofluorescence-NB500-517-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Fyn Antibody (FYN-01) [NB500-517] - Staining of Fyn in human HeLa cell line using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/Fyn-Antibody-FYN-01-Immunocytochemistry-Immunofluorescence-NB500-517-img0003.jpg)
![Immunoprecipitation: Fyn Antibody (FYN-01) [NB500-517] - Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with anti-Fyn (FYN-01). Lane 1: original lysate of T cells Lane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent.](http://images.novusbio.com/fullsize/Fyn-Antibody-FYN-01-Immunoprecipitation-NB500-517-img0001.jpg)
![Immunohistochemistry: CaM Kinase II alpha Antibody [NBP1-51945] - IHC using CamKII antibody from goat in 3% PFA perfused mouse brain. 1:100 dilution clearly provides cytosolic CamK II immunoreactivity in whole brain regions, including the hippocampus (HP), cortex (CTX) and paraventricular region (PVN). Image from verified customer review.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-Immunohistochemistry-NBP1-51945-img0008.jpg "Immunohistochemistry: CaM Kinase II alpha Antibody [NBP1-51945] - IHC using CamKII antibody from goat in 3% PFA perfused mouse brain. 1:100 dilution clearly provides cytosolic CamK II immunoreactivity in whole brain regions, including the hippocampus (HP), cortex (CTX) and paraventricular region (PVN). Image from verified customer review.")
![Flow Cytometry: CaM Kinase II alpha Antibody [NBP1-51945] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-18720231134294.jpg)
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-187202311342929.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - (3.75ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-187202311342911.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - (3.8ug/ml) staining of paraffin embedded Human Brain Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-187202311342919.jpg)
![Western Blot: CaM Kinase II alpha Antibody [NBP1-51945] - (0.3ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-18720231134296.jpg)
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-187202311342912.jpg)
![Western Blot: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of PIM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: BT-20 cell lysateLane 2: HepG2 cell lysate Lane 3: SW480 cell lysate](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Western-Blot-NBP2-67528-img0010.jpg "Western Blot: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of PIM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: BT-20 cell lysateLane 2: HepG2 cell lysate Lane 3: SW480 cell lysate")
![Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunocytochemistry-Immunofluorescence-NBP2-67528-img0009.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human tonsil tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunocytochemistry-Immunofluorescence-NBP2-67528-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunocytochemistry-Immunofluorescence-NBP2-67528-img0008.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human breast carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0002.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human colon cancer tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0003.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human gastric carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0004.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human spleen tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0005.jpg)
![Immunohistochemistry-Paraffin: MST3 Antibody [NBP1-87833] - Staining of human liver, skin, small intestine and testis using Anti-STK24 antibody NBP1-87833 (A) shows similar protein distribution across tissues to independent antibody NBP1-87834 (B).](http://images.novusbio.com/fullsize/MST3-Antibody-Immunohistochemistry-Paraffin-NBP1-87833-img0019.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MST3 Antibody [NBP1-87833] - Staining of human liver, skin, small intestine and testis using Anti-STK24 antibody NBP1-87833 (A) shows similar protein distribution across tissues to independent antibody NBP1-87834 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: MST3 Antibody [NBP1-87833] - Analysis using Anti-STK24 antibody NBP1-87833 (A) shows similar pattern to independent antibody NBP1-87834 (B).](http://images.novusbio.com/fullsize/MST3-Antibody-Western-Blot-NBP1-87833-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MST3 Antibody [NBP1-87833] - Staining of human cell line A-431 shows localization to nucleoli & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MST3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87833-img0008.jpg)
![Immunohistochemistry-Paraffin: MST3 Antibody [NBP1-87833] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/MST3-Antibody-Immunohistochemistry-Paraffin-NBP1-87833-img0022.jpg)
![Western Blot: MST3 Antibody [NBP1-87833] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/MST3-Antibody-Western-Blot-NBP1-87833-img0006.jpg)
![Immunohistochemistry-Paraffin: MST3 Antibody [NBP1-87833] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MST3-Antibody-Immunohistochemistry-Paraffin-NBP1-87833-img0020.jpg)
![Immunohistochemistry-Paraffin: MST3 Antibody [NBP1-87833] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MST3-Antibody-Immunohistochemistry-Paraffin-NBP1-87833-img0021.jpg)
![Immunohistochemistry-Paraffin: MST3 Antibody [NBP1-87833] - Staining of human liver shows very weak positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/MST3-Antibody-Immunohistochemistry-Paraffin-NBP1-87833-img0023.jpg)
![ELISA: MST3 Antibody [NBP1-87833] - MST-3 levels in SH-SY5Y cell lysates. ELISA image added from a verified customer review](http://images.novusbio.com/fullsize/MST3-Antibody-ELISA-NBP1-87833-img0024.jpg)
![Western Blot: PDK3 Antibody [NBP1-32581] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PDK3-Antibody-Western-Blot-NBP1-32581-img0010.jpg "Western Blot: PDK3 Antibody [NBP1-32581] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PDK3 Antibody [NBP1-32581] - Ca922 xenograft, using PDK3 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/PDK3-Antibody-Immunohistochemistry-Paraffin-NBP1-32581-img0004.jpg)
![Western Blot: PDK3 Antibody [NBP1-32581] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:500.](http://images.novusbio.com/fullsize/PDK3-Antibody-Western-Blot-NBP1-32581-img0006.jpg)
![Western Blot: PDK3 Antibody [NBP1-32581] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:1000.](http://images.novusbio.com/fullsize/PDK3-Antibody-Western-Blot-NBP1-32581-img0007.jpg)
![Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Western-Blot-NBP1-47839-img0020.jpg "Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK4.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0013.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded mouse spleen tissue using MKK4/MEK4 antibody (OTI8A8). Image from verified customer review.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0022.jpg)
![Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEK4 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Flow-Cytometry-NBP1-47839-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of HepG2 cells using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0012.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0014.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0015.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human pancreas tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0017.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0018.jpg)
![Immunoprecipitation: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunoprecipitation-NBP1-47839-img0021.jpg)