| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-284SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59337-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12899-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30395-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03109-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22410-0.025mg | |||
![Western Blot: WNK4 Antibody [NB600-284] - Western Blot Image of anti-WNK4. Whole cell protein from Hek293 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WNK4 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence](http://images.novusbio.com/fullsize/WNK4-Antibody-Western-Blot-NB600-284-img0001.jpg "Western Blot: WNK4 Antibody [NB600-284] - Western Blot Image of anti-WNK4. Whole cell protein from Hek293 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WNK4 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence")
![Western Blot: Maxi Potassium channel beta Antibody [NB300-535] - Analysis of Maxi K+ Beta (KCNMB1) was performed by loading 20ug of Movas whole cell lysates with and without Maxi K+ Beta (KCNMB1) siRNA and 10ul prestained protein ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk/TBST for at least 1 hour at room temperature. Maxi K+ Beta was detected using a Maxi K+ Beta rabbit polyclonal antibody at a concentration of 1ug/ml in blocking buffer overnight at 4 degrees C on a rocking platform, followed by a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed.](http://images.novusbio.com/fullsize/Maxi-Potassium-channel-beta-Antibody-Western-Blot-NB300-535-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Maxi Potassium channel beta Antibody [NB300-535] - Analysis of Maxi K+ Beta (KCNMB1) was performed by loading 20ug of Movas whole cell lysates with and without Maxi K+ Beta (KCNMB1) siRNA and 10ul prestained protein ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk/TBST for at least 1 hour at room temperature. Maxi K+ Beta was detected using a Maxi K+ Beta rabbit polyclonal antibody at a concentration of 1ug/ml in blocking buffer overnight at 4 degrees C on a rocking platform, followed by a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Maxi Potassium channel beta Antibody [NB300-535] - analysis of Maxi K+ Beta (green) in Movas cells transfected with (+) and without (-) Maxi K+ Beta siRNA. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a p38 polyclonal antibody at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at a dilution of 1:1000 for 1 hour at room temperature (green). F-actin (red) was stained by Dylight 554 Phalloidin and Nuclei (blue) were stained with Hoechst dye.](http://images.novusbio.com/fullsize/Maxi-Potassium-channel-beta-Antibody-Immunofluorescence-NB300-535-img0004.jpg)
![Western Blot: Maxi Potassium channel beta Antibody [NB300-535] - Analysis of Maxi K+ Beta was performed by loading 20ug of Movas whole cell lysates and 10ul prestained protein ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane u and blocked with 5% Milk/TBST for at least 1 hour at room temperature. Maxi K+ Beta was detected using a Maxi K+ rabbit polyclonal antibody at a concentration of 1ug/ml in blocking buffer overnight at 4 degrees C on a rocking platform, followed by a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed.](http://images.novusbio.com/fullsize/Maxi-Potassium-channel-beta-Antibody-Western-Blot-NB300-535-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Maxi Potassium channel beta Antibody [NB300-535] - Analysis of Maxi K+ Beta (green) in Movas cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a Maxi K+ Beta polyclonal antibody at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at a dilution of 1:1000 for 1 hour at room temperature (green). F-actin (red) was stained by Dylight 554 Phalloidin and Nuclei (blue) were stained with Hoechst dye.](http://images.novusbio.com/fullsize/Maxi-Potassium-channel-beta-Antibody-Immunofluorescence-NB300-535-img0003.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Western-Blot-NBP3-13161-img0006.jpg "Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody [NBP3-13161] - KCC2/SLC12A5 antibody detects KCC2/SLC12A5 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: KCC2/SLC12A5 protein stained by KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13161-img0003.jpg)
![Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody [NBP3-13161] - KCC2/SLC12A5 antibody detects KCC2/SLC12A5 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: KCC2/SLC12A5 protein stained by KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Immunohistochemistry-Paraffin-NBP3-13161-img0002.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Western-Blot-NBP3-13161-img0001.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Western-Blot-NBP3-13161-img0004.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Western-Blot-NBP3-13161-img0005.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13161_rabbit-kcc2-slc12a5-pab-91020242091487.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13161_rabbit-kcc2-slc12a5-pab-9102024200103.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13161_rabbit-kcc2-slc12a5-pab-91020242014613.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Human brain (5 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13161_rabbit-kcc2-slc12a5-pab-910202420134099.jpg)
![Immunohistochemistry: Kv3.1 [p Ser503] Antibody [NB300-279] - IHC staining of medial nucleus of the trapezoid body (MNTB) cells with the phospho-Ser503 Kv3.1 subunit antibody. The left panel shows control cells. The right panel shows cells that have been exposed to the protein kinase C activator PMA.](http://images.novusbio.com/fullsize/Kv3.1-[p-Ser503]-Antibody-Immunohistochemistry-NB300-279-img0001.jpg "Immunohistochemistry: Kv3.1 [p Ser503] Antibody [NB300-279] - IHC staining of medial nucleus of the trapezoid body (MNTB) cells with the phospho-Ser503 Kv3.1 subunit antibody. The left panel shows control cells. The right panel shows cells that have been exposed to the protein kinase C activator PMA.")
![Western Blot: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Western Blot analysis of Rat brain membrane lysate showing detection of KCC2/SLC12A5 protein using Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody, Clone S1-12 (NBP2-59337). Primary Antibody: Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody (NBP2-59337) at 1:1000.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-S1-12-Western-Blot-NBP2-59337-img0005.jpg "Western Blot: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Western Blot analysis of Rat brain membrane lysate showing detection of KCC2/SLC12A5 protein using Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody, Clone S1-12 (NBP2-59337). Primary Antibody: Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody (NBP2-59337) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody, Clone S1-12 (NBP2-59337). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody (NBP2-59337) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) KCC2/SLC12A5 Antibody (D) Composite.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-S1-12-Immunocytochemistry-Immunofluorescence-NBP2-59337-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-S1-12-Immunocytochemistry-Immunofluorescence-NBP2-59337-img0002.jpg)
![Western Blot: Kv7.4 Antibody (S43/6) [NBP2-12899] - Western Blot analysis of Rat tissue lysate showing detection of Kv7.4 protein using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Kv7-4-Antibody-S43-6-Western-Blot-NBP2-12899-img0008.jpg "Western Blot: Kv7.4 Antibody (S43/6) [NBP2-12899] - Western Blot analysis of Rat tissue lysate showing detection of Kv7.4 protein using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Kv7.4 Antibody (S43/6) [NBP2-12899] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kv7.4 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Kv7-4-Antibody-S43-6-Immunocytochemistry-Immunofluorescence-NBP2-12899-img0010.jpg)
![Immunohistochemistry: Kv7.4 Antibody (S43/6) [NBP2-12899] - Immunohistochemistry analysis using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Kv7-4-Antibody-S43-6-Immunohistochemistry-NBP2-12899-img0009.jpg)
![Immunohistochemistry-Frozen: Kv7.4 Antibody (S43/6) [NBP2-12899] - Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 uL for 5 minutes at RT.](http://images.novusbio.com/fullsize/Kv7-4-Antibody-S43-6-Immunohistochemistry-Frozen-NBP2-12899-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Potassium Channel Kv3.1 Antibody [NBP2-30395] - Staining of human cell line U-2 OS shows localization to nucleus, nuclear membrane and cytosol. Antibody staining shown in green.](http://images.novusbio.com/fullsize/Potassium-Channel-Kv3.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30395-img0008.jpg "Immunocytochemistry/Immunofluorescence: Potassium Channel Kv3.1 Antibody [NBP2-30395] - Staining of human cell line U-2 OS shows localization to nucleus, nuclear membrane and cytosol. Antibody staining shown in green.")
![Immunohistochemistry-Paraffin: Potassium Channel Kv3.1 Antibody [NBP2-30395] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/Potassium-Channel-Kv3.1-Antibody-Immunohistochemistry-Paraffin-NBP2-30395-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Potassium Channel Kv3.1 Antibody [NBP2-30395] - Analysis in human cerebral cortex and kidney tissues. Corresponding KCNC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Potassium-Channel-Kv3.1-Antibody-Immunohistochemistry-Paraffin-NBP2-30395-img0009.jpg)
![Immunohistochemistry-Paraffin: Potassium Channel Kv3.1 Antibody [NBP2-30395] - Staining of human small intestine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Potassium-Channel-Kv3.1-Antibody-Immunohistochemistry-Paraffin-NBP2-30395-img0010.jpg)
![Immunohistochemistry-Paraffin: Potassium Channel Kv3.1 Antibody [NBP2-30395] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in granular layer.](http://images.novusbio.com/fullsize/Potassium-Channel-Kv3.1-Antibody-Immunohistochemistry-Paraffin-NBP2-30395-img0011.jpg)
![Immunohistochemistry-Paraffin: Potassium Channel Kv3.1 Antibody [NBP2-30395] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Potassium-Channel-Kv3.1-Antibody-Immunohistochemistry-Paraffin-NBP2-30395-img0013.jpg)
![Western Blot: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25541_rabbit-kcc2-slc12a5-mab-hl2535-azide-and-bsa-free-212024935557.jpg "Western Blot: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - Unboiled and boiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25541_rabbit-kcc2-slc12a5-mab-hl2535-azide-and-bsa-free-2120249362734.jpg)
![Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - KCC2 antibody [HL2535] detects KCC2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. KCC2 stained by KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25541_rabbit-kcc2-slc12a5-mab-hl2535-azide-and-bsa-free-2120249351378.jpg)
![Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - KCC2 antibody [HL2535] detects KCC2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. KCC2 stained by KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25541_rabbit-kcc2-slc12a5-mab-hl2535-azide-and-bsa-free-2120249355574.jpg)
![Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody [HL2535] (NBP3-25541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25541_rabbit-kcc2-slc12a5-mab-hl2535-azide-and-bsa-free-western-blot-1411202518435616.jpg)
![KCC2/SLC12A5 antibody [HL2535] detects KCC2/SLC12A5 protein at Glogi by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: KCC2/SLC12A5 stained by KCC2/SLC12A5 antibody [HL2535] (NBP3-25541) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25541_rabbit-kcc2-slc12a5-mab-hl2535-azide-and-bsa-free-immunocytochemistry-immunofluorescence-1411202518294119.jpg)
![Western Blot: Kv11.1 Antibody [NBP3-03109] - Analysis of extracts of various cell lines, using Kv11.1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhan](http://images.novusbio.com/fullsize/Kv11.1-Antibody-Western-Blot-NBP3-03109-img0004.jpg "Western Blot: Kv11.1 Antibody [NBP3-03109] - Analysis of extracts of various cell lines, using Kv11.1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhan")
![Immunohistochemistry-Paraffin: Kv11.1 Antibody [NBP3-03109] - Human lung cancer using KCNH2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kv11.1-Antibody-Immunohistochemistry-Paraffin-NBP3-03109-img0003.jpg)
![Immunohistochemistry-Paraffin: Kv11.1 Antibody [NBP3-03109] - human breast cancer using KCNH2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kv11.1-Antibody-Immunohistochemistry-Paraffin-NBP3-03109-img0001.jpg)
![Immunohistochemistry-Paraffin: Kv11.1 Antibody [NBP3-03109] - Human colon carcinoma using KCNH2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kv11.1-Antibody-Immunohistochemistry-Paraffin-NBP3-03109-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Kv11.1 Antibody - Azide and BSA Free [Kv11.1] - Immunofluorescence analysis of Mouse kidney tissue using Kv11.1 Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-03109_rabbit-polyclonal-kv11-1-antibody-52202516293929.jpg)
![Western Blot: KCC2/SLC12A5 [p Ser940] Antibody [NBP2-29513] - Rat hippocampal homogenate showing specific labeling of the ~ 135k KCC2 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/KCC2-SLC12A5-[p-Ser940]-Antibody-Western-Blot-NBP2-29513-img0002.jpg "Western Blot: KCC2/SLC12A5 [p Ser940] Antibody [NBP2-29513] - Rat hippocampal homogenate showing specific labeling of the ~ 135k KCC2 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).")
![Boiled and unboiled rat tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Maxi Potassium channel alpha antibody [HL3164] (NBP3-48775) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48775_rabbit-maxi-potassium-channel-alpha-mab-hl3164-western-blot-1411202518431320.jpg "<b>Orthogonal Strategies Validation. </b>Boiled and unboiled rat tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Maxi Potassium channel alpha antibody [HL3164] (NBP3-48775) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Maxi Potassium channel alpha antibody [HL3164] (NBP3-48775) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48775_rabbit-maxi-potassium-channel-alpha-mab-hl3164-western-blot-1411202518404311.jpg)
![Maxi Potassium channel alpha antibody [HL3164] detects Maxi Potassium channel alpha protein by immunofluorescent analysis.Sample: DIV11 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Maxi Potassium channel alpha stained by Maxi Potassium channel alpha antibody [HL3164] (NBP3-48775) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48775_rabbit-maxi-potassium-channel-alpha-mab-hl3164-immunocytochemistry-immunofluorescence-1411202518382819.jpg)
![<b>Orthogonal Strategies Validation. </b>Unboiled various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Maxi Potassium channel alpha antibody [HL3164] (NBP3-48775) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48775_rabbit-maxi-potassium-channel-alpha-mab-hl3164-western-blot-14112025183202.jpg)
![Western Blot: VGCNL1 Antibody (S187-7) [NBP2-22410] - Western Blot analysis of Rat Brain showing detection of ~200 kDa VGCNL1protein using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Western-Blot-NBP2-22410-img0002.jpg "Western Blot: VGCNL1 Antibody (S187-7) [NBP2-22410] - Western Blot analysis of Rat Brain showing detection of ~200 kDa VGCNL1protein using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.")
![Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGCNL1Antibody (D) Composite.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Immunocytochemistry-Immunofluorescence-NBP2-22410-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Immunocytochemistry-Immunofluorescence-NBP2-22410-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VGCNL1Antibody. (D) Composite.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Immunocytochemistry-Immunofluorescence-NBP2-22410-img0004.jpg)